Exogenous Melatonin Enhances Cold, Salt and Drought Stress Tolerance by Improving Antioxidant Defense in Tea Plant (Camellia sinensis (L.) O. Kuntze)

Springer Science and Business Media LLC - Tập 24 Số 9 - Trang 1826
Jiahao Li1, Yiqing Yang1, Kang Sun1, Yi Chen1, Xuan Chen1, Xinghui Li1
1Tea Research Institute, Nanjing Agricultural University, Nanjing, 210095, China

Tóm tắt

Melatonin is a biological hormone that plays crucial roles in stress tolerance. In this study, we investigated the effect of exogenous melatonin on abiotic stress in the tea plant. Under cold, salt and drought stress, increasing malondialdehyde levels and decreasing maximum photochemical efficiency of PSII were observed in tea leaves. Meanwhile, the levels of reactive oxygen species (ROS) increased significantly under abiotic stress. Interestingly, pretreatment with melatonin on leaves alleviated ROS burst, decreased malondialdehyde levels and maintain high photosynthetic efficiency. Moreover, 100 μM melatonin-pretreated tea plants showed high levels of glutathione and ascorbic acid and increased the activities of superoxide dismutase, peroxidase, catalase and ascorbate peroxidase under abiotic stress. Notably, melatonin treatments can positively up-regulate the genes (CsSOD, CsPOD, CsCAT and CsAPX) expression of antioxidant enzyme biosynthesis. Taken together, our results confirmed that melatonin protects tea plants against abiotic stress-induced damages through detoxifying ROS and regulating antioxidant systems.

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Tài liệu tham khảo

Chen, 2009, Differentiation of eight tea (Camellia sinensis) cultivars in China by elemental fingerprint of their leaves, J. Sci. Food Agric., 89, 2350, 10.1002/jsfa.3716

Wang, 2017, Identification of a novel bZIP transcription factor in Camellia sinensis as a negative regulator of freezing tolerance in transgenic arabidopsis, Ann. Bot., 119, 1195, 10.1093/aob/mcx011

Li, 2018, Freezing stress deteriorates tea quality of new flush by inducing photosynthetic inhibition and oxidative stress in mature leaves, Sci. Hortic., 230, 155, 10.1016/j.scienta.2017.12.001

Xiong, 2002, Cell Signaling during Cold, Drought, and Salt Stress, Plant Cell, 14, 165, 10.1105/tpc.000596

Shi, 2015, Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of improved abiotic stress resistance in bermudagrass [Cynodon dactylon (L). Pers.] by exogenous melatonin, J. Exp. Bot., 66, 681, 10.1093/jxb/eru373

Zhou, 2014, Exogenous abscisic acid significantly affects proteome in tea plant (Camellia sinensis) exposed to drought stress, Hortic. Res., 1, 14029, 10.1038/hortres.2014.29

Hou, 2010, Effects of cold acclimation and exogenous pytohormone abscisic acid treatment on physiological indicators of winterness wheat, J. Plant Sci., 5, 125, 10.3923/jps.2010.125.136

Meloni, 2003, Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress, Environ. Exp. Bot., 49, 69, 10.1016/S0098-8472(02)00058-8

Dahal, 2012, The effects of phenotypic plasticity on photosynthetic performance in winter rye, winter wheat and Brassica napus, Physiol. Plant., 144, 169, 10.1111/j.1399-3054.2011.01513.x

Li, 2017, Exogenous Melatonin Confers Salt Stress Tolerance to Watermelon by Improving Photosynthesis and Redox Homeostasis, Front. Plant Sci., 8, 295

Chen, 2018, Exogenous melatonin enhances salt stress tolerance in maize seedlings by improving antioxidant and photosynthetic capacity, Physiol. Plant., 164, 349, 10.1111/ppl.12737

Mittler, 2004, Reactive oxygen gene network of plants, Trends Plant Sci., 9, 490, 10.1016/j.tplants.2004.08.009

Woo, 2004, The Delayed Leaf Senescence Mutants of Arabidopsis, ore1, ore3, and ore9 are Tolerant to Oxidative Stress, Plant Cell Physiol., 45, 923, 10.1093/pcp/pch110

Allakhverdiev, 2008, Heat stress: An overview of molecular responses in photosynthesis, Photosynth. Res., 98, 541, 10.1007/s11120-008-9331-0

Marta, 2016, Exogenous Melatonin Improves Antioxidant Defense in Cucumber Seeds (Cucumis sativus L.) Germinated under Chilling Stress, Front. Plant Sci., 7, 575, 10.3389/fpls.2016.00575

Ding, F., Wang, G., and Zhang, S. (2018). Exogenous Melatonin Mitigates Methyl Viologen-Triggered Oxidative Stress in Poplar Leaf. Molecules, 23.

Calvo, 2013, The role of melatonin in the cells of the innate immunity: A review, J. Pineal Res., 55, 103, 10.1111/jpi.12075

Hattori, 1995, Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates, Biochem. Mol. Biol. Int., 35, 627

Dubbels, 1995, Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry, J. Pineal Res., 18, 28, 10.1111/j.1600-079X.1995.tb00136.x

Manchester, 2015, Melatonin: An ancient molecule that makes oxygen metabolically tolerable, J. Pineal Res., 59, 403, 10.1111/jpi.12267

Wang, 2013, Long-term exogenous application of melatonin delays drought-induced leaf senescence in apple, J. Pineal Res., 54, 292, 10.1111/jpi.12017

Zhang, 2015, Roles of melatonin in abiotic stress resistance in plants, J. Exp. Bot., 66, 647, 10.1093/jxb/eru336

Gao, W., Zhang, Y., Feng, Z., Bai, Q., He, J., and Wang, Y. (2018). Effects of Melatonin on Antioxidant Capacity in Naked Oat Seedlings under Drought Stress. Molecules, 23.

Nawaz, 2016, Melatonin: Current Status and Future Perspectives in Plant Science, Front. Plant Sci., 6, 1230, 10.3389/fpls.2015.01230

Arnao, 2014, Melatonin: Plant growth regulator and/or biostimulator during stress?, Trends Plant Sci., 19, 789, 10.1016/j.tplants.2014.07.006

Arnao, 2017, Melatonin and its relationship to plant hormones, Ann. Bot., 121, 195, 10.1093/aob/mcx114

Wei, 2015, Melatonin enhances plant growth and abiotic stress tolerance in soybean plants, J. Exp. Bot., 66, 695, 10.1093/jxb/eru392

Annia, 2013, On the free radical scavenging activities of melatonin’s metabolites, AFMK and AMK, J. Pineal Res., 54, 245, 10.1111/jpi.12010

Tan, 2007, Novel rhythms of N1-acetyl-N2-formyl-5-methoxykynuramine and its precursor melatonin in water hyacinth: importance for phytoremediation, FASEB J., 21, 1724, 10.1096/fj.06-7745com

Wang, 2012, Delayed senescence of apple leaves by exogenous melatonin treatment: toward regulating the ascorbate–glutathione cycle, J. Pineal Res., 53, 11, 10.1111/j.1600-079X.2011.00966.x

Zhang, 2014, Melatonin: A well-documented antioxidant with conditional pro-oxidant actions, J. Pineal Res., 57, 131, 10.1111/jpi.12162

Zhang, 2014, Melatonin promotes seed germination under high salinity by regulating antioxidant systems, ABA and GA4 interaction in cucumber (Cucumis sativus L.), J. Pineal Res., 57, 269, 10.1111/jpi.12167

Sharif, R., Xie, C., Zhang, H., Arnao, M.B., Ali, M., Ali, Q., Muhammad, I., Shalmani, A., Nawaz, M.A., and Chen, P. (2018). Melatonin and Its Effects on Plant Systems. Molecules, 23.

Janas, 2013, Melatonin, an underestimated natural substance with great potential for agricultural application, Acta Physiol. Plant., 35, 3285, 10.1007/s11738-013-1372-0

Kolodziejczyk, 2016, Melatonin—A new plant biostimulator?, J. Elementol., 21, 1187

Reiter, 2015, Phytomelatonin: Assisting plants to survive and thrive, Molecules, 20, 7396, 10.3390/molecules20047396

Arnao, 2015, Functions of melatonin in plants: A review, J. Pineal Res., 59, 133, 10.1111/jpi.12253

Posmyk, 2009, Melatonin in plants, Acta Physiol. Plant., 31, 1, 10.1007/s11738-008-0213-z

Sarropoulou, 2012, Melatonin enhances root regeneration, photosynthetic pigments, biomass, total carbohydrates and proline content in the cherry rootstock PHL-C (Prunus avium × Prunus cerasus), Plant Physiol. Biochem., 61, 162, 10.1016/j.plaphy.2012.10.001

Li, 2018, Alleviation of cold damage by exogenous application of melatonin in vegetatively propagated tea plant (Camellia sinensis (L.) O. Kuntze), Sci. Hortic., 238, 356, 10.1016/j.scienta.2018.04.068

Kusaba, 2007, Rice NON-YELLOW COLORING1 is involved in light-harvesting complex II and grana degradation during leaf senescence, Plant Cell, 19, 1362, 10.1105/tpc.106.042911

Fei, 2017, Exogenous melatonin ameliorates cold-induced damage in tomato plants, Sci. Hortic., 219, 264, 10.1016/j.scienta.2017.03.029

Fan, 2015, Alleviation of cold damage to photosystem II and metabolisms by melatonin in Bermudagrass, Front. Plant Sci., 6, 925, 10.3389/fpls.2015.00925

Li, 2012, The mitigation effects of exogenous melatonin on salinity-induced stress in Malus hupehensis, J. Pineal Res., 53, 298, 10.1111/j.1600-079X.2012.00999.x

Zhang, 2012, Melatonin promotes water-stress tolerance, lateral root formation, and seed germination in cucumber (Cucumis sativus L.), J. Pineal Res., 54, 15, 10.1111/j.1600-079X.2012.01015.x

Zhao, 2016, Melatonin Increases the Chilling Tolerance of Chloroplast in Cucumber Seedlings by Regulating Photosynthetic Electron Flux and the Ascorbate-Glutathione Cycle, Front. Plant Sci., 7, 1814, 10.3389/fpls.2016.01814

Wang, 2016, Exogenous melatonin improves growth and photosynthetic capacity of cucumber under salinity-induced stress, Photosynthetica, 54, 19, 10.1007/s11099-015-0140-3

Jiang, 2016, Melatonin improves antioxidant capacity and ion homeostasis and enhances salt tolerance in maize seedlings, Acta Physiol. Plant., 38, 82, 10.1007/s11738-016-2101-2

Zhang, 2014, The RNA-seq approach to discriminate gene expression profiles in response to melatonin on cucumber lateral root formation, J. Pineal Res., 56, 39, 10.1111/jpi.12095

Nagalakshmi, 2001, Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus, Plant Sci., 160, 291, 10.1016/S0168-9452(00)00392-7

Kocsy, 2001, Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants, Physiol. Plant., 113, 158, 10.1034/j.1399-3054.2001.1130202.x

Foyer, 2011, Ascorbate and Glutathione: The Heart of the Redox Hub, J. Plant Physiol., 155, 2, 10.1104/pp.110.167569

Wu, 2017, PEG-simulated drought stress and spike in vitro culture are used to study the impact of water stress on barley malt quality, Plant Growth Regul., 81, 243, 10.1007/s10725-016-0201-z

Cui, 2016, Responses ofIn vitro-Grown Plantlets (Vitis vinifera) toGrapevine leafroll-Associated Virus-3and PEG-Induced Drought Stress, Front. Physiol., 7, 203, 10.3389/fphys.2016.00203

Li, 2015, Carbon dioxide enrichment alleviates heat stress by improving cellular redox homeostasis through an ABA-independent process in tomato plants, Plant Biol., 17, 81, 10.1111/plb.12211

Hodges, 1999, Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds, Planta, 207, 604, 10.1007/s004250050524

Willekens, 1997, Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants, EMBO J., 16, 4806, 10.1093/emboj/16.16.4806

Elstner, 1976, Inhibition of nitrite formation from hydroxylammoniumchloride: A simple assay for superoxide dismutase, Anal. Biochem., 70, 616, 10.1016/0003-2697(76)90488-7

Pereira, 2002, Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea, Plant Soil, 239, 123, 10.1023/A:1014951524286

Griffith, 1980, Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine, Anal. Biochem., 106, 207, 10.1016/0003-2697(80)90139-6

Logan, 1998, Seasonal differences in xanthophyll cycle characteristics and antioxidantsin Mahonia repens growing in different light environments, Oecologia, 116, 9

Livak, 2012, Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method, Methods, 25, 402, 10.1006/meth.2001.1262