Excitatory GABA Responses in Embryonic and Neonatal Cortical Slices Demonstrated by Gramicidin Perforated-Patch Recordings and Calcium Imaging

Journal of Neuroscience - Tập 16 Số 20 - Trang 6414-6423 - 1996
David F. Owens1, Leslie H. Boyce2, Marion B. E. Davis2, Arnold R. Kriegstein2
1Department of Neurology, College of Physicians and Surgeons of Columbia University, New York, New York 10025, USA.
2Department of Neurology and The Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York, New York 10025

Tóm tắt

Gramicidin perforated-patch-clamp recordings in brain slices were used to obtain an accurate assessment of the developmental change in the GABAAreceptor reversal potential (EGABAA) in embryonic and early postnatal rat neocortical cells including neuroepithelial precursor cells, cortical plate neurons, and postnatal neocortical neurons. Our results demonstrate that there is a progressive negative shift inEGABAA, with the most positive values found in the youngest cortical precursor cells. At the early stages of neocortical development,EGABAAis determined by the chloride (Cl−) gradient, and the internal chloride concentration ([Cl−]i) decreases with development.EGABAAis positive to the resting potential, indicating that GABA serves to depolarize developing neocortical cells. Consistent with this conclusion, GABAAreceptor activation with muscimol was found to increase the internal calcium concentration ([Ca2+]i) in both embryonic and early postnatal neocortical cells through the activation of voltage-gated calcium channels (VGCCs). Postnatal cells exhibit spontaneous postsynaptic synaptic currents, which are eliminated by bicuculline methiodide (BMI) but not glutamate receptor antagonists and reverse at the Cl−equilibrium potential. Likewise, brief spontaneous increases in [Ca2+]i, sensitive to BMI and TTX, are observed at the same ages, suggesting that endogenous synaptic GABAAreceptor activation can depolarize cells and activate VGCCs. These results suggest that GABAAreceptor-mediated depolarization may influence early neocortical developmental events, including neurogenesis and synaptogenesis, through the activation of Ca2+-dependent signal transduction pathways.

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Journal of Neuroscience

Tập 16 Số 20

6414-6423

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