Examples of chemical modification and recombinant DNA approaches with hemoglobin

Fantl, 1987, Specifically carboxymethylated hemoglobin as an analogue of carbamino hemoglobin: Solution and x-ray studies of carboxymethylated hemoglobin and x-ray studies of carbamino hemoglobin, J. Biol. Chem., 262, 12700, 10.1016/S0021-9258(18)45263-5

Fantl, 1987, Properties of carboxymethylated, cross-linked hemoglobin A, Biochemistry, 26, 5755, 10.1021/bi00392a026

Nigen, 1980, Oxygen-linked binding sites for inorganic anions to hemoglobin, J. Biol. Chem., 255, 5525, 10.1016/S0021-9258(19)70661-9

Arnone, 1972, X-ray diffraction study of binding of 2,3-diphosphoglycerate to human deoxyhaemoglobin, Nature, 237, 146, 10.1038/237146a0

Ueno, 1992, The functional, oxygen-linked chloride binding sites of hemoglobin are contiguous within a channel in the central cavity, J. Prot. Chem., 11, 177, 10.1007/BF01025223

Ueno, 1994, Random chemical modification of the oxygen-linked chloride binding sites of hemoglobin: Those in the central dyad axis may influence the transition between deoxy- and oxy-hemoglobin, J. Prot. Chem., 12, 561, 10.1007/BF01025120

Perutz, 1994, The chloride effect in human haemoglobin. A new kind of allosteric mechanism, J. Mol. Biol., 239, 555, 10.1006/jmbi.1994.1394

Wagenbach, 1991, Synthesis of wild-type and mutant human hemoglobins in Saccharomyces cerevisiae, BioTechnology, 9, 57, 10.1038/nbt0191-57

Martin de Llano, 1993, Biochemical and functional properties of recombinant human sickle hemoglobin expressed in yeast, J. Biol. Chem., 268, 27004, 10.1016/S0021-9258(19)74210-0

Yanase, 1995, A recombinant human hemoglobin with asparagine-102 (β) substituted by alanine has a limiting low oxygen affinity, reduced marginally by chloride, Protein Sci., 4, 21, 10.1002/pro.5560040104