Evolution of the synaptonemal complex in Helix aspersa spermatocytes

Chromosoma - Tập 36 Số 2 - Trang 150-157 - 1972
Esponda, P.1, Stockert, J. C.1
1Departamento de Citología, Instituto de Biología Celular, Madrid

Tóm tắt

In spermatocytes of Helix aspersa, the structure of the synaptonemal complexes undergoes changes in the course of the pachytene, the lateral elements being transformed into wide bands of lesser density than the chromatin. By using the uranyl-EDTA-lead sequence, which preferentially stains RNA, the lateral elements can be made to appear positive in the early pachytene while the corresponding areas, which become wider and more diffuse, are positive during late pachytene. — Apparently, the lateral elements do not persist in the diplotene and remnants of the central element can occasionally be observed. Using the uranyl-EDTA-lead method reveals some positively stained material surrounding the chromatin, mostly granular in appearance, which is observed in late pachytene and attains its maximum amount during diplotene. Several aspects of these observations are here discussed.

Tài liệu tham khảo

citation_journal_title=J. Cell Sci.; citation_title=The fine structure of oogonia and oocytes in human ovaries; citation_author=T.G. Baker, L.L. Franchi; citation_volume=2; citation_publication_date=1967; citation_pages=213-224; citation_id=CR1 citation_journal_title=Chromosoma (Berl.); citation_title=The structure of the chromosomes in human primordial oocytes; citation_author=T.G. Baker, L.L. Franchi; citation_volume=22; citation_publication_date=1967; citation_pages=358-377; citation_id=CR2 citation_journal_title=J. Ultrastruct. Res.; citation_title=A new staining procedure for electron microscopical cytology; citation_author=W. Bernhard; citation_volume=27; citation_publication_date=1969; citation_pages=250-265; citation_id=CR3 citation_title=The biochemistry of development; citation_publication_date=1960; citation_id=CR4; citation_author=J. Brachet; citation_publisher=Pergamon Press citation_journal_title=Chromosoma (Berl.); citation_title=Whole mount electron microscopy of meiotic chromosomes and the synaptonemal complex; citation_author=D.E. Comings, T.A. Okada; citation_volume=30; citation_publication_date=1970; citation_pages=269-286; citation_id=CR5 citation_journal_title=Nat. Cancer Inst. Monogr.; citation_title=Nucleolar RNA synthesis during mitotic and meiotic prophase; citation_author=N. Das, M. Alfert; citation_volume=26; citation_publication_date=1966; citation_pages=337-351; citation_id=CR6 citation_journal_title=J. Ultrastruct. Res.; citation_title=Localization of RNA in the synaptinemal complex; citation_author=P. Esponda, J.C. Stockert; citation_volume=35; citation_publication_date=1971; citation_pages=411-417; citation_id=CR7 citation_journal_title=Proc. roy. Soc. B; citation_title=The ultrastructure of oogonia and oocytes in the foetal and neonatal rat; citation_author=L.L. Franchi, A.M. Mandl; citation_volume=157; citation_publication_date=1963; citation_pages=99-114; citation_id=CR8 citation_journal_title=Chromosoma (Berl.); citation_title=Synaptinemal complexes in the achiasmatic spermatogenesis of Bolbe nigra Giglio-Tos (Mantoidea); citation_author=G. Gassner; citation_volume=26; citation_publication_date=1969; citation_pages=22-34; citation_id=CR9 citation_journal_title=Chromosoma (Berl.); citation_title=RNA synthesis during male meiosis and spermatogenesis; citation_author=S.A. Henderson; citation_volume=15; citation_publication_date=1964; citation_pages=345-366; citation_id=CR10 citation_journal_title=Chromosoma (Berl.); citation_title=Meiotic prophase in anthers of asynaptic wheat; citation_author=L.F. Cour, B. Wells; citation_volume=29; citation_publication_date=1970; citation_pages=419-427; citation_id=CR11 citation_journal_title=Chromosoma (Berl.); citation_title=The structure and function of synaptinemal complexes in Lilium longiflorum sporocytes; citation_author=P.B. Moens; citation_volume=23; citation_publication_date=1968; citation_pages=418-451; citation_id=CR12 citation_journal_title=J. Cell Biol.; citation_title=Multiple core complexes in grasshopper spermatocytes and spermatids; citation_author=P.B. Moens; citation_volume=40; citation_publication_date=1969; citation_pages=542-551; citation_id=CR13 citation_journal_title=Chromosoma (Berl.); citation_title=Differential rate of ribonucleic acid synthesis in the autosomes and sex chromosomes during meiosis in the mouse; citation_author=V. Monesi; citation_volume=17; citation_publication_date=1965; citation_pages=11-21; citation_id=CR14 citation_journal_title=Ann. Rev. Genet.; citation_title=Synaptinemal complex; citation_author=M.J. Moses; citation_volume=2; citation_publication_date=1968; citation_pages=363-412; citation_id=CR15 citation_journal_title=Nuclear physiology and differentiation. Genetics, Suppl.; citation_title=Structure and function of the synaptonemal complex; citation_author=M.J. Moses; citation_volume=61; citation_publication_date=1969; citation_pages=41-51; citation_id=CR16 citation_title=Lampbrush pachytene chromosomes of pigeon after enzyme treatment; citation_inbook_title=Fifth Intern. Congr. Electron Microsc.; citation_publication_date=1962; citation_pages=20-8; citation_id=CR17; citation_author=B.R. Nebel; citation_publisher=Academic Press citation_journal_title=Chromosoma (Berl.); citation_title=Enzyme effects on pachytene chromosomes of male pigeon evaluated with the electron microscope; citation_author=B.R. Nebel, E.M. Coulon; citation_volume=13; citation_publication_date=1962; citation_pages=292-299; citation_id=CR18 citation_journal_title=J. Ultrastruct. Res.; citation_title=Cytochemical studies on chromosome ultrastructure; citation_author=W.F. Sheridan, R.J. Barrnett; citation_volume=27; citation_publication_date=1969; citation_pages=216-230; citation_id=CR19 citation_journal_title=Chromosoma (Berl.); citation_title=The behaviour of chromosomal axes during diplotene in mouse spermatocytes; citation_author=A.J. Solari; citation_volume=31; citation_publication_date=1970; citation_pages=217-230; citation_id=CR20 citation_title=Ultrastructure of the chromosomes at meiosis; citation_inbook_title=Handbook of molecular cytology; citation_publication_date=1969; citation_pages=412; citation_id=CR21; citation_author=J.R. Sotelo; citation_publisher=North-Holland Publ. Co. citation_journal_title=C. R. Lab. Carlsberg; citation_title=Studies on the mechanisms of crossing over. IV. The molecular organization of the synaptinemal complex, in Neottiella (Cooke) Saccardo (Ascomycetes); citation_author=M. Westergaard, D. Wettstein; citation_volume=37; citation_publication_date=1970; citation_pages=239-268; citation_id=CR22