Sự tiến hóa của các cấu trúc thứ cấp RNA ribosome ITS ở các sinh vật cộng sinh nấm và tảo của một số loài thuộc giống Cladonia sect. Cladonia (Cladoniaceae, Ascomycotina)

Journal of Molecular Evolution - Tập 64 - Trang 528-542 - 2007
Sara Beiggi1, Michele D. Piercey-Normore1
1Department of Botany, University of Manitoba, Winnipeg, Canada

Tóm tắt

Các nghiên cứu tiến hóa trong các hiệp hội địa y theo chân sinh vật cộng sinh nấm (mycobiont), là sinh vật cộng sinh mà địa y được đặt tên và chiếm phần lớn thallus. Tuy nhiên, sự tiến hóa của đối tác tảo (photobiont) cũng rất quan trọng để duy trì tính tương thích giữa các sinh vật cộng sinh và tối ưu hóa năng suất của hiệp hội địa y. Các vùng spacer nội bộ (ITS) của DNA ribosome hạt nhân (rDNA) đã được nghiên cứu để xác định các mẫu trình tự DNA chính và các mẫu trong cấu trúc thứ cấp của các trình tự rRNA ở cả hai sinh vật cộng sinh thuộc giống Cladonia. Các sinh vật cộng sinh nấm và tảo cho thấy những xu hướng trái ngược về tốc độ tiến hóa và chiều dài đoạn. Cả hai sinh vật cộng sinh đều cho thấy sự bảo tồn mạnh mẽ hơn của cấu trúc ITS2 so với cấu trúc ITS1. Sự tương đồng là rõ ràng trong các cấu trúc thứ cấp giữa hai nhóm thuỷ sinh chi diệp và nấm ascomycete khác nhau. Hầu hết các loài nấm và tất cả các phức hợp loài đều phi đa nguồn gốc. ITS rDNA của tảo địa y tự nhiên từ Manitoba và bốn loài tảo đã biết có độ tương đồng cao. Tảo địa y tự nhiên phân tách thành các nhánh được hỗ trợ mạnh bởi các đặc điểm môi trường, cho thấy rằng tảo đã thích nghi với môi trường có thể kết hợp với các hạt giống nấm đang nảy mầm thuộc giống Cladonia. Tính linh hoạt của nấm có thể cho phép mycobiont thích nghi với môi trường của photobiont, tạo ra sự biến đổi trong hình thái địa y. Điều này có thể giải thích sự không tương đồng của các mẫu phát sinh chủng loại giữa các đối tác tảo và nấm đã được thử nghiệm và sự đa nguồn gốc của các loài nấm.

Từ khóa

#địa y #tảo #nấm #mycobiont #photobiont #ITS #rDNA #cấu trúc thứ cấp

Tài liệu tham khảo

Ahmadjian V (1987) Coevolution in lichens. Ann NY Acad Sci 503:307–315 Ahti T (1980) Taxonomic revision of Cladonia gracilis and its allies. Ann Bot Fenn 17:195–243 Ahti T (2000) Cladoniaceae, Flora Neotropica Monograph 78. New York Botanical Garden, New York Ahti T, DePriest PT (2001) New combinations of Cladina epithets in Cladonia (Ascomycotina:Cladoniaceae). Mycotaxon 78:499–502 Aptroot A, Sipman HJM, van Herk CM (2001) Cladonia monomorpha, a neglected cup lichen from Europe. Lichenologist 33:271–283 Armaleo D, Clerc P (1991) Lichen chimeras: DNA analysis suggests that one fungus forms two morphotypes. Exp Mycol 15:1–10 Bakker FT, Olsen JL, Stam WT (1995) Evolution of nuclear rDNA ITS sequences in the Cladophora albida/sericea clade (Chlorophyta). J Mol Evol 40:640–651 Beck A (1999) Photobiont inventory of a lichen community growing on heavy-metal-rich rock. Lichenologist 31:501–510 Beck A, Friedl T, Rambold G (1998) Selectivity of photobiont choice in a defined lichen community: inferences from cultural and molecular studies. New Phytol 139:709–720 Brodo IM (1973) Substrate ecology. In Ahmadjian V, Hale ME (eds) The lichens. Academic Press, New York, pp 401–441 Brodo IM, Sharnoff SD, Sharnoff S (2001) Lichens of North America. Yale University Press, London Coleman AW (2003) ITS2 is a double-edged tool for eukaryote evolutionary comparisons. Trends Genet 19:370–375 Coleman AW, Mai JC (1997) Ribosomal DNA ITS-1 and ITS-2 sequence comparisons as a tool for predicting genetic relatedness. J Mol Evol 45:168–177 Coleman AW, Preparata RM, Mehrotra B, Mai JC (1998) Derivation of the secondary structure of the ITS-1 transcript in Volvocales and its taxonomic correlations. Protist 149:135–146 Côté CA, Greer CL, Peculis BA (2002) Dynamic conformational model for the role of ITS2 in pre-rRNA processing in yeast. RNA 8:786–797 Culberson CF (1972) Improved conditions and new data for the identification of lichen products by a standardized thin-layer chromatographic method. J Chromatogr 72:113–125 Culberson CF (1986) Biogenetic relationships of the lichen substances in the framework of systematics. Bryologist 89:91–98 Culberson CF, Culberson WL, Johnson A (1988) Gene flow in lichens. Am J Bot 75:1135–1139 Culberson WL (1986) Chemistry and sibling speciation in the lichen-forming fungi: ecological and biological considerations. Bryologist 89:123–131 Esslinger TL (2006) A cumulative checklist for the lichen-forming, lichenicolous and allied fungi of the continental United States and Canada. North Dakota State University, Fargo; available at: http://www.ndsu.nodak.edu/instruct/esslinge/chcklst/chcklst7htm; accessed 10 April 2006 Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 Freidl T (1987) Thallus development and phycobionts of the parasitic lichen Diploschistes muscorum. Lichenologist 19:183–191 Friedl T (1989) Comparative ultrastructure of pyrenoids in Trebouxia (Microthamniales, Chlorophyta). Plant Syst Evol 164:145–159 Friedl T, Rokitta C (1997) Species relationships in the lichen alga Trebouxia (Chlorophyta, Trebouxiophyceae): molecular phylogenetic analyses of nuclear-encoded large subunit rRNA gene sequences. Symbiosis 23:125–148 Friedl T, Zeltner C (1994) Assessing the relationships of some coccoid green lichen algae and the Microthamniales (Chlorophyta) with 18S ribosomal RNA gene sequence comparisons. J Phycol 30:500–506 Gargas A, DePriest PT, Grube M, Tehler A (1995) Multiple origins oflichen symbioses in fungi suggested by SSU rDNA phylogeny. Science 268(5216):1492–1495 Gilbert OL (1977) Fungal plasticity in Cladonia pocillum. Lichenologist 9:172–173 Goffinet B, Bayer RJ (1997) Characterization of mycobionts of photomorph pairs in the Peltigeraceae (lichenized Ascomycetes) based on internal transcribed spacer sequences of the nucler ribosomal DNA. Fungal Genet Biol 21:228–237 Good L, Intine RVA, Nazar RN (1997) Interdependence in the processing of ribosomal RNAs in Schizosaccharomyces pombe. J Mol Biol 273:782–788 Grube M, DePriest PT, Gargas A, Hafellner J (1995) DNA isolation from lichen ascomata. Mycol Res 99:1321–1324 Hausner G, Wang X (2005) Unusual compact rDNA gene arrangements within some members of the Ascomycota: evidence for molecular co-evolution between ITS1 and ITS2. Genome 48:1–13 Hawksworth DL (1973) Ecological factors and species delimitation in the lichens. In: Heywood VH (ed) Taxonomy and ecology. Academic Press, New York, pp 31–70 Helms G, Friedl T, Rambold G, Mayrhofer H (2001) Identification of photobionts from the lichen family Physciaceae using algal-specific ITS rDNA sequencing. Lichenologist 33:73–86 Hildreth KC, Ahmadjian V (1981) A study of Trebouxia and Pseudotrebouxia isolates from different lichens. Lichenologist 13:65–86 Honegger R (1996) Mycobionts. In: Nash TH III, (ed) Lichen biology. Cambridge University Press, New York, pp 24–36 Janzen DH (1980) When is it coevolution? Evolution 34:611–612 Joseph N, Krauskoph E, Vera MI, Michot B (1999) Ribosomal internal transcribed spacer 2 (ITS2) exhibits a common core of secondary structure in vertebrates and yeast. Nucleic Acids Res 27:4533–4540 Kishino H, Hasagawa M (1989) Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. J Mol Evol 29:170–179 Kroken S, Taylor JW (2000) Phylogenetic species, reproductive mode, and specificity of the green alga Trebouxia forming lichens with the fungal genus Letharia. Bryologist 103:645–660 Lalev AI, Nazar RN (1998) Conserved core structure in the internal transcribed spacer 1 of the Schizosaccharomyces pombe precursor ribosomal RNA. J Mol Biol 284:1341–1351 Lalev AI, Abeyrathne PD, Nazar RN (2000) Ribosomal RNA maturation in Schizosaccharomyces pombe is dependent on a large ribonucleoprotein complex of the internal transcribed spacer 1. J Mol Biol 302:65–77 Laundon JR (1995) On the classification of lichen photomorphs. Taxon 44:387–389 Lott TJ, Burns BM, Zancope-Oliveira R, Elie CM, Reiss E (1998) Sequence analysis of the Internal Transcribed Spacer 2 (ITS2) from yeast species within the genus Candida. Curr Microbiol 36:63–69 Lutzoni FM (1997) Phylogeny of lichen- and non-lichen-forming omphalinoid mushrooms and the utility of testing for combinability among multiple data sets. Syst Biol 46:373–406 Lutzoni F, Pagel M, Reeb V (2001) Major fungal lineages are derived from lichen symbiotic ancestors. Nature 411:937–940 Mai JC, Coleman AW (1997) The internal transcribed spacer 2 exhibits a common secondary structure in green algae and flowering plants. J Mol Evol 44:258–271 Mai JC, Myllys L, Lohtander K, Tehler A (2001) Beta-tubulin, ITS and group I intron sequences challenge the species pair concept in Physcia aipolia and P. caesia. Mycologia 93:335–343 Ohmura Y, Kawachi M, Kasai F, Watanabe MM (2006) Genetic combinations of symbionts in a vegetatively reproducing lichen, Parmotrema tinctorum, based on ITS rDNA sequences. Bryologist 109:43–59 Ott S (1987) Sexual reproduction and developmental adaptations in Xanthoria parietina. Nordic J Bot 7:219–228 Ott S, Meier T, Jahns HM (1995) Development, regeneration, and parasitic interactions between the lichens Fulgensia bracteata and Toninia caeruleonigricans. Can J Bot 73 (Suppl 1):S595–S602 Paterson AM, Gray RD (1997) Host-parasite co-speciation, host switching, and missing the boat. In: Clayton DH, Moore J (eds) Host-parasite evolution. Oxford University Press, New York, pp 236–250 Piercey-Normore MD (2004) Selection of algal partners by lichen fungi and patterns of variation for three species of Cladonia. Can J Bot 82:947–961 Piercey-Normore MD (2006) The lichen-forming ascomycete Evernia mesomorpha associates with multiple genotypes of Trebouxia jamesii. New Phytol 169:331–344 Piercey-Normore MD, DePriest PT (2001) Algal switching among lichen symbioses. Am J Bot 88:1490–1498 Piercey-Normore MD, Coxson D, Goward T, Goffinet B. (2006) Phylogenetic position of a Pacific Northwest North American endemic cyanolichen, Nephroma occultum (Ascomycota, Peltigerales). Lichenologist 38:441–456 Pintado A, Valladares F, Sancho LG (1997) Exploring phenotypic plasticity in the lichen Ramalina capitata: morphology, water relations, and chlorophyll content in north- and south-facing populations. Ann Bot 80:345–353 Purvis OW (1997) The species concept in lichens. In: Claridge MF, Dawah HA, Wilson MR (ed) Species: the units of biodiversity. The systematics association special volume series 54. Chapman and Hall, London, pp 109–134 Rambaut A (2001) Se-Al (sequence alignment editor version 1). Department of Zoology, University of Oxford, Oxford, UK Rikkinen J (1997) Habitat shifts and morphological variation of Pseudevernia furfuracea along a topographical gradient. Symb Bot Ups 32:223–245 Rikkinen J, Oksanen I, Lohtander K (2002) Lichen guilds share related cyanobacterial symbionts. Science 297:357 Sojo F, Valladares F, Sancho LG (1997) Structural and physiological plasticity of the lichen Catillaria corymbosa in different microhabitats of the maritime Antarctica. Bryologist 100:171–179 Stenroos S, Hyvonen J, Myllys L, Thell A, Ahti T (2002) Phylogeny of the genus Cladonia s. lat. (Cladoniaceae, Ascomycetes) inferred from molecular, morphological, and chemical data. Cladistics 18:237–278 Stenroos S, Stocker-Wörgötter E, Yoshimura I, Myllys L, Thell A, Hyvönen J (2003) Culture experiments and DNA sequence data confirm the identity of Lobaria photomorphs. Can J Bot 81:232–247 Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods), version 4. Sinauer Associates, Sunderland, MA Thompson JN (1994) The coevolutionary process. University of Chicago Press, Chicago Thomson JW (1967) The lichen genus Cladonia in North America. University of Toronto Press, Toronto Thomson JW (1984) American Arctic lichens. 1. The macrolichens. Columbia University Press, New York Torres RA, Ganal M, Hamleben V (1990) GC balance in the internal transcribed spacers ITS1 and ITS2 of nuclear ribosomal RNA genes. J Mol Evol 30:170–181 Wedin M, Doring H, Gilenstam G (2004) Saprotrophy and lichenization as options for the same fungal species on different substrata: environmental plasticity and fungal lifestyles in the Stictis-Conotrema complex. New Phytol 164:459–465 White TJ, Bruns TD, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322 Yahr R, Vilgalys R, DePriest PT (2004) Strong fungal specificity and selectivity for algal symbionts in Florida scrub Cladonia lichens. Mol Ecol 13:3367–3378 Zoller S, Lutzoni F (2003) slow algae, fast fungi: exceptionally high nucleotide substitution rate differences between lichenized fungi Omphalina and their symbiotic green algae Coccomyxa. Mol Phylogenet Evol 29:629–640 Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415