Evidence for the presence of numerous protein components in immature bovine dental enamel
Tóm tắt
The proteins and peptides of immature enamel were extracted from freshly slaughtered bovine embryos in solutions containing protease inhibitors. No detectable differences were noted in the number of components, their overall amino acid composition, or molecular weights from the proteins and peptides extracted 12–16 h postmortem in solutions which contained no protease inhibitors. These data indicate that the large number of components found in developing bovine enamel is not due to proteolysis occurring during their isolation. Significant amounts of protein components having molecular weights greater than ∼15,000 were not detected. Therefore, if the ameloblasts initially synthesize only a few high molecular weight protein species, the present data imply that in vivo degradation of the high molecular weight enamel proteins occurs very rapidly after their synthesis and precedes the massive loss of protein which accompanies the final stages of enamel mineralization and maturation.
Tài liệu tham khảo
Bonar, L.C., Glimcher, M.J., Mechanic, G.L.: The molecular structure of the neutral-soluble proteins of embryonic bovine enamel in the solid state. J. Ultrastruct. Res.13, 308–317 (1965)
Bonar, L.C., Mechanic, G.L., Glimcher, M.J.: Optical rotatory dispersion studies of the neutral soluble proteins of embryonic bovine enamel. J. Ultrastruct. Res.13, 296–307 (1965)
Burgess, R.C., Maclaren, C.: Proteins in developing bovine enamel. In: Tooth enamel: Its composition, properties, and fundamental structure (M.V. Stack and R.W. Fearnhead, eds.), pp. 74–82, Bristol: Wright 1965
Craig, L.C.: Techniques for the study of peptides and proteins by dialysis and diffusion. Methods in enzymology11, 870–905 (1967)
Deakins, M.: Changes in the ash, water, and organic content of pig enamel during calcification. J. Dent. Res.21, 429–435 (1942).
Eastoe, J.E.: The amino acid composition of proteins from oral tissues: II. The matrix proteins in dentine and enamel from developing human deciduous teeth. Arch. oral Biol.8, 633–652 (1963)
Eggert, F.M., Allen, G.R., Burgess, R.C.: Purification and partial characterization of proteins from developing bovine dental enamel. Biochem. J.131, 471–484 (1973)
Elwood, W.K., Apostolopoulos, A.X.: Analysis of developing enamel of the rat: II. Electrophoretic and amino acid studies. Calcif. Tiss. Res.17, 327–335 (1975)
Fincham, A.G.: Electrophoretic and Sephadex gel filtration studies of bovine foetal enamel matrix at acid pH. Calcif. Tiss. Res.2, 353–360 (1968)
Fincham, A.G.: Experiments on the DEAE-cellulose chromatography of bovine foetal enamel matrix and the isolation of a low molecular weight fraction. In: Tooth enamel II: Its composition, properties, and fundamental structure (R.W. Fearnhead, and M.V. Stack, eds.), pp. 79–87. Bristol: Wright 1971
Fukae, M.: Biochemical studies of enamel formation (in Japanese). J. Biochem.37, 295–303 (1970)
Fukae, M., Shimizu, M.: Studies on the proteins of developing bovine enamel. Arch. oral Biol.19, 381–386 (1974)
Fukae, M., Shimokawa, H., Iwakura, M., Suda, T., Sasaki, S.: Studies of the biosynthesis of enamel proteins in the rat by use of3H-proline (abstract). J. Dent. Res.51, 1298 (1972)
Glimcher, M.J., Bonar, L.C., Daniel, E.J.: The molecular structure of the protein matrix of bovine dental enamel. J. Mol. Biol.3, 541–546 (1961)
Glimcher, M.J., Friberg, U.A., Levine, P.T.: The isolation and amino acid composition of the enamel proteins of erupted bovine teeth. Biochem. J.93, 202–210 (1964)
Glimcher, M.J., Levine, P.T.: Studies of the proteins, peptides and free amino acids of mature bovine enamel. Biochem. J.98, 742–753 (1966)
Glimcher, M.J., Mechanic, G., Bonar, L.C., Daniel, E.: The amino acid composition of the organic matrix of decalcified fetal bovine dental enamel. J. Biol. Chem.236, 3210–3213 (1961)
Glimcher, M.J., Mechanic, G.L., Friberg, U.A.: The amino acid composition of the organic matrix and the neutral soluble and acid soluble components of embryonic bovine enamel. Biochem. J.93, 198–202 (1964)
Guenther, H.L., Croissant, R.C., Schonfeld, S., Slavkin, H.C.: Enamel proteins: Identification of epithelial-specific differentiation products. In: Extracellular matrix influences on gene expression (H.C. Slavkin and R.C. Greulich, eds.), pp. 387–398. New York: Academic Press 1975
Katz, E.P., Mechanic, G.L., Glimcher, M.J.: The ultracentrifugal and free zone electrophoretic characterization of the neutral soluble proteins of embryonic bovine enamel. Biochim. Biophys. Acta107, 471–484 (1965)
Katz, E.P., Seyer, J.M., Levine, P.T., Glimcher, M.J.: The comparative biochemistry of the organic matrix of developing enamel: II. Ultracentrifugal and electrophoretic characterization of proteins soluble at neutral pH. Arch. oral Biol.14, 533–539 (1969)
Levine, P.T., Seyer, J.M., Huddleston, J., Glimcher, M.J.: The comparative biochemistry of the organic matrix proteins of developing enamel: I. Amino acid composition. Arch. oral Biol.12, 407–410 (1967)
Mechanic, G.L.: The multicomponent re-equilibrating protein system of bovine embryonic enamelin (dental enamel protein): Chromatography in deaggregating solvents. In: Tooth enamel II. Its composition, properties, and fundamental structure (R.W. Fearnhead and M.V. Stack, eds.), pp. 88–94, Bristol: Wright 1971
Mechanic, G.L., Katz, E.P., Glimcher, M.J.: The sephadex gel filtration characteristics of the neutral soluble proteins of embryonic bovine enamel. Biochim. Biophys. Acta133, 97–113 (1967)
Nikiforuk, G., Gruca, M.: Immunological reactions of the organic matrix of developing bovine enamel. Calcif. Tiss. Res.4, 129–135 (1969)
Nikiforuk, G., Gruca, M.: Immunological and gel-filtration characteristics of bovine enamel protein. In: Tooth enamel II: Its composition, properties, and fundamental structure (R.W. Fearnhead and M.V. Stack, eds.), pp. 95–99. Bristol: Wright 1971
Ornstein, L.: Disc electrophoresis: I. Background and theory. Ann. N.Y. Acad. Sci.121, 321–349 (1964)
Piez, K.A., Morris, L.: A modified procedure for the automatic analysis of amino acids. Anal. Biochem.1, 187–201 (1960)
Seyer, J.M.: Evolution of mineralizing tissues: Bone, dentin and enamel. In: The comparative molecular biology of extracellular matrices (H.C. Slavkin, ed.), pp. 273–284. New York: Academic Press 1972
Seyer, J.M., Glimcher, M.J.: The content and nature of the carbohydrate components of the organic matrix of embryonic bovine enamel. Biochim. Biophys. Acta184, 509–522 (1969)
Seyer, J.M., Glimcher, M.J.: The isolation of phosphorylated polypeptide components of the organic matrix of embryonic bovine enamel. Biochim. Biophys. Acta236, 279–291 (1971)
Shimokawa, H., Sasaki, S.: Biosynthesis of enamel protein in vitro (abstract). J. Dent. Res.54A, 107 (1975)
Stack, M.V.: Chemical organization of the organic matrix of enamel. In: Structural and chemical organization of teeth, Vol. 2. (A.E.W. Miles, ed.), pp. 317–346. New York: Academic Press 1967
Weidmann, S.M., Eyre, D.R.: The protein of mature and foetal enamel. In: Tooth enamel II: Its composition, properties, and fundamental structure (R.W. Fearnhead and M.V. Stack, eds.), pp. 72–78. Bristol: Wright 1971
Weinmann, J.P., Wessinger, G.D. Reed, G.: Correlation of chemical and histological investigations on developing enamel. J. Dent. Res.21, 171–182 (1942)