Bằng chứng về sự thay đổi nhanh chóng liên quan đến thủy triều trong microbiome của san hô Coelastrea aspera

Coral Reefs - Tập 36 - Trang 815-828 - 2017
M. J. Sweet1, B. E. Brown2,3, R. P. Dunne4, I. Singleton2,5, M. Bulling1
1Environmental Sustainability Research Centre, College of Life and Natural Sciences, University of Derby, Derby,, UK
2School of Biology, Newcastle University, Newcastle upon Tyne, UK
3Environmental Research Institute, North Highland College, Caithness, UK
4West Briscoe, Baldersdale, Barnard Castle, UK
5School of Applied Sciences, Sighthill Campus, Edinburgh Napier University, Edinburgh, UK

Tóm tắt

Sự thay đổi trong microbiome của san hô sống ngầm Coelastrea aspera (trước đây được gọi là Goniastrea aspera) từ Phuket, Thái Lan, đã được ghi nhận trong suốt 4 ngày của kỳ thủy triều lớn. Trong thời gian này, san hô đã tự nhiên tiếp xúc với nhiệt độ cao, bức xạ mặt trời mạnh, tiếp xúc dưới không khí và dòng nước thay đổi do thủy triều. Phân tích microbiome 16S đã chỉ ra rằng san hô chứa cả cộng đồng 'cốt lõi hoặc ổn định' và những cộng đồng có vẻ 'tạm thời hoặc sporadic'. Chỉ một số ít các sinh vật liên kết vi sinh vật được phân loại là vi sinh vật cốt lõi; phần lớn là tạm thời hoặc sporadic. Những sinh vật liên kết tạm thời này có thể bị ảnh hưởng bởi sự thay đổi độ dày của dịch nhầy và dòng nước do thủy triều gây ra. Tại đây, chúng tôi báo cáo những thay đổi mạnh mẽ trong cộng đồng vi khuẩn của C. aspera trong một khoảng thời gian ngắn. Tuy nhiên, chúng tôi cũng chỉ ra những khác biệt đáng kể về thời gian thay đổi giữa hai nhóm tuổi của san hô được nghiên cứu. Những thay đổi nhanh chóng (trong vòng 2 ngày tiếp xúc dưới không khí) diễn ra trong các thuộc địa 4 tuổi, nhưng phản ứng hơi chậm hơn được quan sát thấy ở các thuộc địa 10 tuổi, nơi mà các sinh vật liên kết vi sinh chỉ thay đổi sau 4 ngày. Chúng tôi giả thuyết rằng những thay đổi này có liên quan đến tuổi và có thể bị ảnh hưởng bởi những khác biệt cơ bản trong microbiome của san hô 4 và 10 tuổi, cũng như các tương tác giữa vi khuẩn và/hoặc năng lượng của chủ thể.

Từ khóa

#microbiome #Coelastrea aspera #thủy triều #vi sinh vật #nhiệt độ cao #bức xạ mặt trời #cộng đồng vi khuẩn

Tài liệu tham khảo

Ainsworth T, Krause L, Bridge T, Torda G, Raina J-B, Zakrzewski M, Gates RD, Padilla-Gamiño JL, Spalding HL, Smith C, Woolsey ES, Bourne DG, Bongaerts P, Hoegh-Guldberg O, Leggat W (2015) The coral core microbiome identifies rare bacterial taxa as ubiquitous endosymbionts. ISME J 9:2261–2274 Anderson MJ, Walsh DC (2013) PERMANOVA, ANOSIM, and the Mantel test in the face of heterogeneous dispersions: what null hypothesis are you testing? Ecol Monogr 83:557–574 Anthony KRN, Connolly SR, Willis BL (2002) Comparative analysis of energy allocation to tissue and skeletal growth in corals. Limnol Oceanogr 47:1417–1429 Apprill A, Rappé MS (2011) Response of the microbial community to coral spawning in lagoon and reef flat environments of Hawaii, USA. Aquat Microb Ecol 62:251–266 Babcock RC (1991) Comparative demography of three species of scleractinian corals using age- and size-dependent classifications. Ecol Monogr 61:225–244 Bourne DG, Garren M, Work TM, Rosenberg E, Smith GW, Harvell CD (2009) Microbial disease and the coral holobiont. Trends Microbiol 17:554–562 Brown BE, Dunne RP (2008) Solar radiation modulates bleaching and damage protection in a shallow water coral. Mar Ecol Prog Ser 362:99–107 Brown BE, Dunne RP, Scoffin TP, Letissier MDA (1994) Solar damage in intertidal corals. Mar Ecol Prog Ser 105:219–230 Brown BE, Downs CA, Dunne RP, Gibb SW (2002) Exploring the basis of thermotolerance in the reef coral Goniastrea aspera. Mar Ecol Prog Ser 242:119–129 Brown BE, Dunne RP, Phongsuwan N, Somerfield PJ (2011) Increased sea level promotes coral cover on shallow reef flats in the Andaman Sea, eastern Indian Ocean. Coral Reefs 30:867–878 Brown BE, Dunne RP, Phongsuwan N, Patchim L, Hawkridge JM (2014) The reef coral Goniastrea aspera: a “winner” becomes a “loser” during a severe bleaching event in Thailand. Coral Reefs 33:395–401 Carlos C, Torres TT, Ottoboni LMM (2013) Bacterial communities and species-specific associations with the mucus of Brazilian coral species. Sci Rep 3:1–7 Ceh J, Raina JB, Soo RM, van Keulen M, Bourne DG (2012) Coral–bacterial communities before and after a coral mass spawning event on Ningaloo Reef. PLoS One 7:e36920 Charuchinda M, Hylleberg J (1984) Skeletal extension of Acropora formosa at a fringing reef in the Andaman Sea. Coral Reefs 3:215–219 Cho I, Blaser M (2012) The human microbiome: at the interface of health and disease. Nat Rev Genet 13:260–270 Clarke KR, Gorley RN (2006) PRIMER v6: user manual/tutorial. PRIMER-E Ltd, Plymouth, UK de Jonge VN, van Beusekom JEE (1995) Wind- and tide-induced resuspension of sediment and microphytobenthos from tidal flats in the Ems estuary. Limnol Oceanogr 40:776–778 Dirksen P, Marsh SA, Braker I, Heitland N, Wagner S, Nakad R, Mader S, Petersen C, Kowallik V, Rosenstiel P, Félix M-A, Schulenburg H (2016) The native microbiome of the nematode Caenorhabditis elegans: gateway to a new host–microbiome model. BMC Biol 14:38 Ditlev H (1978) Zonation of corals (Scleractinia: Coelenterata) on intertidal reef flats at Ko Phuket, Eastern Indian Ocean. Mar Biol 47:29–39 Glasl B, Herndl GJ, Frade PR (2016) The microbiome of coral surface mucus has a key role in mediating holobiont health and survival upon disturbance. ISME J 10:2280–2292 Hester ER, Barott KL, Nulton J, Vermeij MJ, Rohwer FL (2015) Stable and sporadic symbiotic communities of coral and algal holobionts. ISME J 10:1157–1169 Jatkar AA, Brown BE, Bythell JC, Guppy R, Morris NJ, Pearson JP (2010) Coral mucus: the properties of its constituent mucins. Biomacromolecules 11:883–888 Junjie RK, Browne NK, Erftemeijer PLA, Todd PA (2014) Impacts of sediments on coral energetics: partitioning the effects of turbidity and settling particles. PLoS One 9:e107195 Koren O, Rosenberg E (2006) Bacteria associated with mucus and tissues of the coral Oculina patagonica in summer and winter. Appl Environ Microbiol 72:5254–5259 Krediet CJ, Ritchie KB, Paul VJ, Teplitski M (2013) Coral-associated micro-organisms and their roles in promoting coral health and thwarting diseases. Proc R Soc Lond B Biol Sci 280:20122328 Li K, Bihan M, Methe BA (2013) Analyses of the stability and core taxonomic memberships of the human microbiome. PLoS One 8:e63139 Long RA, Azam F (2001) Antagonistic interactions among marine bacteria. Appl Environ Microbiol 67:4875–4983 McFall-Ngai M, Hadfield MG, Bosch TCG, Carey HV, Domazet-Lošo T, Douglas AE, Dubilier N, Eberl G, Fukami T, Gilbert SF, Hentschel U, King N, Kjelleberg S, Knoll AH, Kremer N, Mazmanian SK, Metcalf JL, Nealson K, Pierce NE, Rawls JF, Reid A, Ruby EG, Rumpho M, Sanders JG, Tautz D, Wernegreen JJ (2013) Animals in a bacterial world, a new imperative for the life sciences. Proc Natl Acad Sci U S A 110:3229–3236 Morrow KM, Moss AG, Chadwick NE, Liles MR (2012) Bacterial associates of two Caribbean coral species reveal species-specific distribution and geographic variability. Appl Environ Microbiol 78:6438–6449 Nakajima R, Yoshida T, Azman BAR, Zaleha K, Othman BHR, Toda T (2009) In situ release of coral mucus by Acropora and its influence on the heterotrophic bacteria. Aquat Ecol 43:815–823 Nedashkovskaya OI, Kim SB, Shin DS, Beleneva IA, Mikhailov VV (2007) Fulvivirga kasyanovii gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from seawater in a mussel farm. Int J Syst Evol Microbiol 57:1046–1049 Nugues MM, Smith GW, van Hooidonk RJ, Seabra MI, Bak RPM (2004) Algal contact as a trigger for coral disease. Ecol Lett 7:919–923 Oksanen JF, Blanchet G, Friendly M, Kindt R, Legendre P, McGlinn D, Minchin PR, O’Hara PR, Simpson GL, Solymos P, Stevens H, Szoecs E, Wagner H (2016) vegan: community ecology package. R package version 2.4-0 Pride DT, Salzman J, Haynes M, Rohwer F, Davis-Long C, White RA, Loomer P, Armitage GC, Relman DA (2012) Evidence of a robust resident bacteriophage population revealed through analysis of the human salivary virome. ISME J 6:915–926 R Core Team (2016) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria Reshef L, Koren O, Loya Y, Zilber-Rosenberg I, Rosenberg E (2006) The coral probiotic hypothesis. Environ Microbiol 8:2068–2073 Ritchie KB (2006) Regulation of microbial populations by coral surface mucus and mucus-associated bacteria. Mar Ecol Prog Ser 322:1–14 Ritchie KB, Jones LE, Ellner SP, Ritchie KB, Jones LE, Ellner SP (2010) How microbial community composition regulates coral disease development. Plos Biol 8:e1000345 Roff DA (2006) Introduction to computer-intensive methods of data analysis in biology. Cambridge University Press, Cambridge, UK Rohwer F, Seguritan V, Azam F, Knowlton N (2002) Diversity and distribution of coral-associated bacteria. Mar Ecol Prog Ser 243:1–10 Rosenberg E, Koren O, Reshef L, Efrony R, Zilber-Rosenberg I (2007) The role of microorganisms in coral health, disease and evolution. Nat Rev Microbiol 5:355–362 Röthig T, Ochsenkühn MA, Roik A, van der Merwe R, Voolstra CR (2016) Long-term salinity tolerance is accompanied by major restructuring of the coral bacterial microbiome. Mol Ecol 25:1308–1323 Rozas M, Enríquez R (2014) Piscirickettsiosis and Piscirickettsia salmonis in fish: a review. J Fish Dis 37:163–188 Schmitt S, Tsai P, Bell J, Fromont J, Ilan M, Lindquist N, Perez T, Rodrigo A, Schupp PJ, Vacelet J, Webster N, Hentschel U, Taylor MW (2012) Assessing the complex sponge microbiota: core, variable and species-specific bacterial communities in marine sponges. ISME J 6:564–576 Scoffin TP, Tudhope AW, Brown BE, Chansang H, Cheeney RF (1992) Patterns and possible environmental controls of skeletogenesis of Porites lutea, South Thailand. Coral Reefs 11:1–11 Scoffin TP, Brown BE, Dunne RP, LeTissier MDA (1997) The controls on growth form of intertidal massive corals, Phuket, south Thailand. Palaios 12:237–248 Shade A, Handelsman J (2012) Beyond the Venn diagram: the hunt for a core microbiome. Environ Microbiol 14:4–12 Smith DC, Douglas AE (1987) The biology of symbiosis. Edward Arnold (Publishers) Ltd Sweet MJ, Bulling MT (2017) On the importance of the microbiome and pathobiome in coral health and disease. Front Mar Sci 4:9 Sweet MJ, Croquer A, Bythell JC (2010) Temporal and spatial patterns in waterborne bacterial communities of an island reef system. Aquat Microb Ecol 61:1–11 Sweet MJ, Bythell JC, Nugues MM (2013) Algae as reservoirs for coral pathogens. PLoS One 8:e69717 Thompson JR, Rivera HE, Closek CJ, Medina M (2014) Microbes in the coral holobiont: partners through evolution, development, and ecological interactions. Front Cell Infect Microbiol 4:176 Turlapati SA, Minocha R, Bhiravarasa PS, Tisa LS, Thomas WK, Minocha SC (2013) Chronic N-amended soils exhibit an altered bacterial community structure in Harvard Forest, MA, USA. FEMS Microbiol Ecol 83:478–493 Turnbaugh PJ, Gordon JI (2009) The core gut microbiome, energy balance and obesity. J Physiol 587:4153–4158 van Oppen M, Baker A, Coffroth M, Willis B (2009) Bleaching resistance and the role of algal endosymbionts. In: van Oppen M, Lough J (eds) Coral bleaching: patterns, processes, causes and consequences. Springer, Berlin, Heidelberg, pp 83–102 Williams AD, Brown BE, Putchim L, Sweet MJ (2015) Age-relateds shifts in bacterial diversity in a reef coral. PLoS One 10:e0144902