Even illumination in total internal reflection fluorescence microscopy using laser light

Microscopy Research and Technique - Tập 71 Số 1 - Trang 45-50 - 2008
Reto Fiolka1, Yury Belyaev1, Helge Ewers2, Andreas Stemmer1
1Nanotechnology Group, ETH Zurich, Tannenstrasse 3, CH‐8092 Zurich, Switzerland
2Institute of Biochemistry, ETH Zurich, Schafmattstr. 18, CH-8093 Zurich, Switzerland

Tóm tắt

AbstractIn modern fluorescence microscopy, lasers are a widely used source of light, both for imaging in total internal reflection and epi‐illumination modes. In wide‐field imaging, scattering of highly coherent laser light due to imperfections in the light path typically leads to nonuniform illumination of the specimen, compromising image analysis. We report the design and construction of an objective‐launch total internal reflection fluorescence microscopy system with excellent evenness of specimen illumination achieved by azimuthal rotation of the incoming illuminating laser beam. The system allows quick and precise changes of the incidence angle of the laser beam and thus can also be used in an epifluorescence mode. Microsc. Res. Tech., 2008. © 2007 Wiley‐Liss, Inc.

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Tài liệu tham khảo

10.1038/nature01686

10.1034/j.1600-0854.2001.21104.x

Axelrod D, 2003, Total internal reflection fluorescence microscopy in cell biology. Biophotonics, Part B, 1

10.1364/AO.18.002679

Ellis GW, 1988, An annular scan phase‐contrast scanned aperture microscope (Aspsam), Cell Motil Cytoskeleton, 10, 342

Mattheyses AL, 2005, Effective elimination of laser interference fringing in fluorescence microscopy by spinning azimuthal incidence angle, Biophys J, 88, 341A

10.1117/1.2161018

10.1088/0022-3727/38/10/R01

10.1038/35074539

10.1038/nature03866

10.1016/S0006-3495(00)76808-1

10.1016/j.copbio.2004.12.004

Smith AE, 2004, Alternate endocytic pathways revealed by virus entry into animal cells, Mol Biol Cell, 15, 195A

10.1016/S0006-3495(99)77382-0

10.1046/j.1365-2818.2003.01200.x

10.1083/jcb.149.1.33

Thông tin
Thông tin xuất bản

Microscopy Research and Technique

Tập 71 Số 1

45-50

Thông tin tác giả