Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food

Sensors - Tập 9 Số 7 - Trang 5810-5824
Angela M. Valadez1, Carlos Lana2,3, Shu‐I Tu4,5, Mark T. Morgan6, Arun K. Bhunia6
1Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907, USA; E-Mails: (A.M.V); (M.T.M).
2 E-Mails: [email protected] (A.M.V)
3School of Aeronautics & Astronautics, Purdue University, 701 W. Stadium Ave., West Lafayette, Indiana 47907, USA
5United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA
6Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907, USA

Tóm tắt

Salmonella enterica is a major food-borne pathogen of world-wide concern. Sensitive and rapid detection methods to assess product safety before retail distribution are highly desirable. Since Salmonella is most commonly associated with poultry products, an evanescent wave fiber-optic assay was developed to detect Salmonella in shell egg and chicken breast and data were compared with a time-resolved fluorescence (TRF) assay. Anti-Salmonella polyclonal antibody was immobilized onto the surface of an optical fiber using biotin-avidin interactions to capture Salmonella. Alexa Fluor 647-conjugated antibody (MAb 2F-11) was used as the reporter. Detection occurred when an evanescent wave from a laser (635 nm) excited the Alexa Fluor and the fluorescence was measured by a laser-spectrofluorometer at 710 nm. The biosensor was specific for Salmonella and the limit of detection was established to be 103 cfu/mL in pure culture and 104 cfu/mL with egg and chicken breast samples when spiked with 102 cfu/mL after 2–6 h of enrichment. The results indicate that the performance of the fiber-optic sensor is comparable to TRF, and can be completed in less than 8 h, providing an alternative to the current detection methods.

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Sensors

Tập 9 Số 7

5810-5824

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