Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food
Tóm tắt
Salmonella enterica is a major food-borne pathogen of world-wide concern. Sensitive and rapid detection methods to assess product safety before retail distribution are highly desirable. Since Salmonella is most commonly associated with poultry products, an evanescent wave fiber-optic assay was developed to detect Salmonella in shell egg and chicken breast and data were compared with a time-resolved fluorescence (TRF) assay. Anti-Salmonella polyclonal antibody was immobilized onto the surface of an optical fiber using biotin-avidin interactions to capture Salmonella. Alexa Fluor 647-conjugated antibody (MAb 2F-11) was used as the reporter. Detection occurred when an evanescent wave from a laser (635 nm) excited the Alexa Fluor and the fluorescence was measured by a laser-spectrofluorometer at 710 nm. The biosensor was specific for Salmonella and the limit of detection was established to be 103 cfu/mL in pure culture and 104 cfu/mL with egg and chicken breast samples when spiked with 102 cfu/mL after 2–6 h of enrichment. The results indicate that the performance of the fiber-optic sensor is comparable to TRF, and can be completed in less than 8 h, providing an alternative to the current detection methods.
Từ khóa
Tài liệu tham khảo
DuPont, 2007, Food safety: the growing threat of foodborne bacterial enteropathogens of animal origin, Clin. Infect. Dis, 45, 1353, 10.1086/522662
Isaacs, 2005, An international outbreak of salmonellosis associated with raw almonds contaminated with a rare phage type of Salmonella enteritidis, J. Food Prot, 68, 191, 10.4315/0362-028X-68.1.191
Centers for Disese and Prevention (2009). Multistate outbreak of Salmonella infections associated with peanut butter and peanut butter-containing products - United States, 2008–2009. MMWR, 58, 85–90.
Humphrey, 2004, Science and society - Salmonella, stress responses and food safety, Nat. Rev. Microbiol, 2, 504, 10.1038/nrmicro907
Santos, 2003, Pathogenesis of Salmonella-induced enteritis, Brazilian J. Med. Biol. Res, 36, 3, 10.1590/S0100-879X2003000100002
Altekruse, 2006, Salmonella Enteritidis in broiler chickens, United States, 2000–2005, Emerg. Infect. Dis, 12, 1848, 10.3201/eid1212.060653
Swaminathan, 2006, Surveillance for human Salmonella infections in the United States, J. AOAC Int, 89, 553
Rodrigue, 1990, International increase in Salmonella enteritidis - a new pandemic, Epidemiol. Infect, 105, 21, 10.1017/S0950268800047609
Centers for Disese and Prevention (1996). Multidrug-resistant Salmonella serotype Typhimurium - United States, 1996. MMWR, 46, 308–310.
Bhunia, 2008, Biosensors and bio-based methods for the separation and detection of foodborne pathogens, Adv. Food Nutr. Res, 54, 1, 10.1016/S1043-4526(07)00001-0
Leonard, 2003, Advances in biosensors for detection of pathogens in food and water, Enz. Microb. Technol, 32, 3, 10.1016/S0141-0229(02)00232-6
Deisingh, 2004, Biosensors for the detection of bacteria, Can. J. Microbiol, 50, 69, 10.1139/w03-095
Taitt, 2005, Evanescent wave fluorescence biosensors, Biosens. Bioelectron, 20, 2470, 10.1016/j.bios.2004.10.026
Leung, 2007, A review of fiber-optic biosensors, Sens. Actuat. B: Chem, 125, 688, 10.1016/j.snb.2007.03.010
Bhunia, A.K., Geng, T., Lathrop, A.A., Valadez, A., and Morgan, M.T. (, January April,). Optical immunosensors for detection of Listeria monocytogenes and Salmonella Enteritidis from food. Orlando, FL, USA.
Anderson, 2002, Improved fluoroimmunoassays using the dye Alexa Fluor 647 with the RAPTOR, a fiber optic biosensor, J. Immunol. Methods, 271, 17, 10.1016/S0022-1759(02)00327-7
Kramer, 2004, A rapid and automated fiber optic-based biosensor assay for the detection of Salmonella in spent irrigation water used in the sprouting of sprout seeds, J. Food Prot, 67, 46, 10.4315/0362-028X-67.1.46
Ko, 2006, A novel FRET-based optical fiber biosensor for rapid detection of Salmonella typhimurium, Biosens. Bioelectron, 21, 1283, 10.1016/j.bios.2005.05.017
DeMarco, 1999, Rapid detection of Escherichia coli O157:H7 in ground beef using a fiber-optic biosensor, J. Food Prot, 62, 711, 10.4315/0362-028X-62.7.711
Geng, 2006, Fiber-optic biosensor employing Alexa-Fluor conjugated antibody for detection of Escherichia coli O157:H7 from ground beef in four hours, Sensors, 6, 796, 10.3390/s6080796
Geng, 2004, Detection of low levels of Listeria monocytogenes cells by using a fiber-optic immunosensor, Appl. Environ. Microbiol, 70, 6138, 10.1128/AEM.70.10.6138-6146.2004
Nanduri, 2006, Antibody immobilization on waveguides using a flow-through system shows improved Listeria monocytogenes detection in an automated fiber optic biosensor: RAPTOR (TM), Sensors, 6, 808, 10.3390/s6080808
Diamandis, E.P., and Christopoulos, T.K. (1996). Immunoassay, Academic Press.
Tu, 2002, The use of time-resolved fluoroimmunoassay to simultaneously detect Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Enteriditis in foods, J. Rapid Methods Autom. Microbiol, 10, 37, 10.1111/j.1745-4581.2002.tb00008.x
Tu, 2003, Rapid detection of outbreak Escherichia coli O157 and Salmonella on alfalfa sprouts by immunomagnetic capture and time-resolved fluorescence, J. Food Safety, 23, 75, 10.1111/j.1745-4565.2003.tb00353.x
Tu, 2004, Time-resolved fluorescence detection of Shiga-like toxins produced by Escherichia coli O157 and non-O157 in ground beef, J. Rapid Methods Autom. Microbiol, 12, 247, 10.1111/j.1745-4581.2004.12405.x
Peruski, 2002, Rapid and sensitive detection of biological warfare agents using time-resolved fluorescence assays, J. Immunol. Methods, 263, 35, 10.1016/S0022-1759(02)00030-3
Valadez, A.M. (2006). Development of a fiber-optic waveguide biosensor assay for the detection of Salmonella Enteritidis in food, Purdue University. M.S. Thesis.
Tu, 2005, Detection of Escherichia coli O157:H7 through the formation of sandwiched complexes with immunomagnetic and fluorescent beads, J. Rapid Methods Autom. Microbiol, 13, 269, 10.1111/j.1745-4581.2005.00026.x
Tu, 2007, Detection of Salmonella Enteritidis in shell egg contents by immunomagnetic capture and time-resolved fluorescence, J. Rapid Methods Autom. Microbiol, 15, 107, 10.1111/j.1745-4581.2007.00081.x
Jaradat, 2004, Optimization of a rapid dot-blot immunoassay for detection of Salmonella enterica serovar Enteritidis in poultry products and environmental samples, Food Microbiol, 21, 761, 10.1016/j.fm.2004.01.010