Evaluation of the Taguchi methods for the simultaneous assessment of the effects of multiple variables in the tumour microenvironment

International Seminars in Surgical Oncology - 2004
Hisham Morsi1, Kwee L Yong2, Andrew P Jewell1
1School of Life Sciences, Kingston University, Kingston upon Thames, UK
2Department of Haematology, University College London Medical School, London, UK

Tóm tắt

The control of proliferation, differentiation and survival of normal and malignant cells in the tumour microenvironment is under the control of a wide range of different factors, including cell:cell interactions, cytokines, growth factors and hormonal influences. However, the ways in which these factors interact are poorly understood. In order to compare the effects of multiple variables, experimental design becomes complex and difficult to manage. We have therefore evaluated the use of a novel approach to multifactorial experimental design, the Taguchi methods, to approach this problem. The Taguchi methods are widely used by quality engineering scientists to compare the effects of multiple variables, together with their interactions, with a simple and manageable experimental design. In order to evaluate these methods, we have used a simple and robust system to compare a traditional experimental design with the Taguchi Methods. The effect of G-CSF, GM-CSF, IL3 and M-CSF on daunorubicin mediated cytotoxicity in K562 cells was measured using the MTT assay. Both methods demonstrated that the same combination of growth factors at the same concentrations minimised daunorubicin cytotoxicity in this assay. These findings demonstrate that Taguchi methods may be a valuable tool for the investigation of the interactions of multiple variables in the tumour microenvironment.

Từ khóa


Tài liệu tham khảo

Peace GS: Taguchi Methods: A Hands-on Approach. 1993, Addison-Wesley

Cobb BD, Clarkson JM: A Simple Procedure For Optimizing The Polymerase Chain Reaction PCR Using Modified Taguchi Methods. Nucleic Acids Research. 1994, 22: 3801-3805.

Caetano-Anolles G: DAF optimization using Taguchi methods and the effect of thermal cycling parameters on DNA amplification. Biotechniques. 1998, 25: 472-6.

Burch GJ, Ferguson CH, Cartwright G, Kwong FY: Application Of Taguchi Experimental Design To The Optimization Of A Baculovirus Expression System. Biochem Soc Trans. 1995, 23: 1075-abstract

Mburu G, Aspden RM, Hutchinson JD: Optimising The Configuration Of Cement Keyholes For Acetabular Fixation In Total Hip Replacement Using Taguchi Experimental Design. Proceedings Of The Institution Of Mechanical Engineers Part H- Journal Of Engineering In Medicine. 1998, 213: 485-492. 10.1243/0954411991535095.

Jeney C, Dobay O, Lengyel A, Adam E, Nasz I: Taguchi Optimisation Of ELISA Procedures. Journal Of Immunological Methods. 1998, 223: 137-146. 10.1016/S0022-1759(98)00185-9.

Taner MT, Antony J: Reassessment of the CAGE questionnaire by ROC/Taguchi methods. Int J Technol Assess Health Care. 2004, 20: 242-246. 10.1017/S0266462304001035.

Taner MT, Antony J: The assessment of quality in medical diagnostic tests: a comparison of ROC/Youden and Taguchi methods. Int J Health Care Qual Assur Inc Leadersh Health Serv. 2000, 13: 300-7. 10.1108/09526860010378744.

Thông tin
Thông tin xuất bản

International Seminars in Surgical Oncology

Thông tin tác giả