Evaluation of the Lipid Content in Bovine Oocytes and Embryos with Nile Red: a Practical Approach

Reproduction in Domestic Animals - Tập 40 Số 1 - Trang 76-78 - 2005
Jo Leroy1, G. Genicot2, Isabelle Donnay2, Ann Van Soom1
1Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine; Ghent University; Salisburylaan, Merelbeke Belgium
2Catholic University of Louvain, Institut des Sciences de la Vie, Unité des Sciences Vétérinaires, Louvain‐la‐Neuve, Belgium

Tóm tắt

ContentsIn this study, the fluorescent lipid dye Nile Red, was used to demonstrate that the lipid content of immature bovine oocytes is correlated with the morphological appearance of the ooplasm. Oocytes with a uniform dark cytoplasm contained significantly more intracellular lipids in lipid droplets compared with oocytes with a granulated or pale cytoplasm (p < 0.05). Furthermore, this lipid‐analysing technique was applied for the first time on single bovine in vitro embryos, showing a significant increase of the lipid content in lipid droplets after culture in the presence of serum (p < 0.05).

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Reproduction in Domestic Animals

Tập 40 Số 1

76-78

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