Evaluation of the C‐terminal C5a effector site with short synthetic C5a analog peptides

European Journal of Immunology - Tập 23 Số 3 - Trang 646-652 - 1993
Jörg Köhl1, Bettina Lübbers1, Andreas Klos1, Wilfried Bautsch1, M Casaretto2
1Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Hannover
2Deutsches Wollforschungs-institut Aachen, Aachen

Tóm tắt

AbstractBiological activities have been determined for a series of 18 peptides based on the C‐terminal sequence of human or rat C5a. Lysosomal enzyme release was tested in two cell types, the promyelotic leukemia cell line U937 and polymorphonuclear leukocytes. In addition, an ATP‐release assay with guinea pig platelets was performed. It was demonstrated that the C‐terminal octapeptide 67‐74 of human C5a represents the minimal sequence required to induce a measurable biological signal in all assays. Extending this peptide to a length of 21 amino acids produced at best only a slight enhancement of potency. Amino acid replacements with either tryptophanyl or phenylalanyl residues in positions between 65–69 either increased potency (at position 67), or abrogated potency (at position 66) in the two lysosomal enzyme assays. N‐terminal acylation with the fluorenylmethoxy‐carbonyl‐aminohexanoyl group slightly enhanced C5a potency. In desensitization experiments with guinea pig platelets all peptides with a C5a activity were able to desensitize not only the C5a but also the C3a responses.

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Tài liệu tham khảo

Bitter‐Suermann D., 1988, The Complement System, 367

Gallin J. I., 1988, Inflammation, 55

Smedegard G., 1989, Am. J. Pathol., 135, 489

10.1172/JCI112506

10.1016/0022-4804(89)90055-3

10.1126/science.3992245

10.1002/prot.340030302

10.1021/bi00427a025

10.1515/bchm2.1980.361.2.1389

Gerard C. G., 1979, J. Reticuloendothel. Soc., 26, 711

10.1016/0161-5890(80)90067-X

Kahn S. A. Erickson B. W. Kawahara M. S.andHugli T. E. J. KöhlB.Lübbers A. Kloset al.

10.1073/pnas.86.1.292

Edalji R. P., 1987, Fed. Proc., 46, 980

10.1021/jm00111a022

10.1021/bi00229a003

Gerardy‐Schahn R., 1988, Biochem. J., 255, 209

10.1002/eji.1830200709

Zanker B., 1982, Agents Actions (Suppl.), 11, 147

Bitter‐Suermann D., 1986, Prog. Allergy, 39, 134

10.1016/S0171-2985(11)80316-5

10.1021/bi00491a024

10.1084/jem.155.3.698

10.1016/S0021-9258(18)94252-3

10.1515/bchm3.1989.370.1.217

10.1021/ja00722a043

10.1248/cpb.29.2825

10.1021/jm00080a004

Caporale L. H., 1980, J. Biol. Chem., 255, 10758, 10.1016/S0021-9258(19)70372-X

10.1042/bj2880261

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European Journal of Immunology

Tập 23 Số 3

646-652

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