Evaluation of serum level of substance P and tissue distribution of NK-1 receptor in endometrial cancer

Springer Science and Business Media LLC - Tập 45 - Trang 2257-2262 - 2018
Naghmeh Gharaee1, Leila Pourali2, Amir Hossein Jafarian3, Seyed Isaac Hashemy4
1Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Obstetrics and Gynecology, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Pathology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Tóm tắt

Substance P (SP), a neuropeptide belonging to the tachykinin family, exerts different biological activities mainly through neurokinin-1 receptor (NK1R). The role of SP/NK1R system in tumoral growth and spread is reported in several cancers. We aimed to evaluate the serum SP concentration and NK1R tissue distribution in endometrial cancer, and to study the relationship between these factors with tumor size, lymph node involvement, disease stage and cancer grade. Recruiting 22 patients with endometrial cancer and 21 patients with leiomyoma as the control group, serum SP concentration was measured using an ELISA method, and NK1R tissue distributions were immunohistochemically analyzed. Serum SP concentration in patients was significantly higher than the control group (p-value = 0.005). The expression level of NK1R in tumoral tissue was more than normal tissue (p-value < 0.001). The NK1R expression had a significant relationship with lymph node involvement (p-value = 0.005) and disease stage (p-value = 0.017). The NK1R expression was higher in more advanced and less-differentiated tumors. SP/NK1R system seems to play a role in tumor growth and development in endometrial cancer. As well, the NK1R expression increased in endometrial cancer, and may be considered as a prognostic factor; but further studies are needed in this field.

Tài liệu tham khảo

Severini C, Improta G, Falconieri-Erspamer G, Salvadori S, Erspamer V (2002) The tachykinin peptide family. Pharmacol Rev 54(2):285–322 Steinhoff MS, von Mentzer B, Geppetti P, Pothoulakis C, Bunnett NW (2014) Tachykinins and their receptors: contributions to physiological control and the mechanisms of disease. Physiol Rev 94(1):265–301. https://doi.org/10.1152/physrev.00031.2013 Gaddum J (1931) An unidentified depressor substance in certain tissue extracts. J Physiol 72(1):74–87 Garcia-Recio S, Gascón P (2015) Biological and pharmacological aspects of the NK1-Receptor. BioMed Res Int. https://doi.org/10.1155/2015/495704 Datar P, Srivastava S, Coutinho E, Govil G (2004) Substance P: structure, function, and therapeutics. Curr Top Med Chem 4(1):75–103 Bossaller C, Reither K, Hehlert-Friedrich C, Auch-Schwelk W, Graf K, Grafe M, Fleck E (1992) In vivo measurement of endothelium-dependent vasodilation with substance P in man. Herz 17(5):284–290 Eklund B, Jogestrand T, Pernow B (1977) Effect of substance P on resistance and capacitance vessels in the human forearm. In: von Euler US, Pernow B (eds) Substance P. Raven Press, New York Shimizu Y, Matsuyama H, Shiina T, Takewaki T, Furness J (2008) Tachykinins and their functions in the gastrointestinal tract. Cell Mol Life Sci 65(2):295–311 Saito R, Takano Y, Kamiya HO (2003) Roles of substance P and NK(1) receptor in the brainstem in the development of emesis. J Pharmacol Sci 91(2):87–94 Fields HL, Levine JD (1984) Pain–mechanics and management. West J Med 141(3):347–357 Klassert TE, Patel SA, Rameshwar P (2010) Tachykinins and neurokinin receptors in bone marrow functions: neural-hematopoietic link. J Recept Ligand Channel Res 3:51–61 Nabzdyk LP, Kuchibhotla S, Guthrie P, Chun M, Auster ME, Nabzdyk C, Deso S, Andersen N, Gnardellis C, LoGerfo FW (2013) Expression of neuropeptides and cytokines in a rabbit model of diabetic neuroischemic wound healing. J Vasc Surg 58(3):766–775 (e712) Rosso M, Robles-Frías MJ, Coveñas R, Salinas-Martín MV, Muñoz M (2008) The NK-1 receptor is expressed in human primary gastric and colon adenocarcinomas and is involved in the antitumor action of L-733,060 and the mitogenic action of substance P on human gastrointestinal cancer cell lines. Tumor Biol 29(4):245–254 Friess H, Zhu Z, Liard V, Shi X, Shrikhande SV, Wang L, Lieb K, Korc M, Palma C, Zimmermann A (2003) Neurokinin-1 receptor expression and its potential effects on tumor growth in human pancreatic cancer. Lab Investig 83(5):731–742 Singh D, Joshi DD, Hameed M, Qian J, Gascón P, Maloof PB, Mosenthal A, Rameshwar P (2000) Increased expression of preprotachykinin-I and neurokinin receptors in human breast cancer cells: implications for bone marrow metastasis. Proc Natl Acad Sci USA 97(1):388–393 Munoz M, Gonzalez-Ortega A, Rosso M, Robles-Frias MJ, Carranza A, Salinas-Martin MV, Covenas R (2012) The substance P/neurokinin-1 receptor system in lung cancer: focus on the antitumor action of neurokinin-1 receptor antagonists. Peptides 38(2):318–325. https://doi.org/10.1016/j.peptides.2012.09.024 Muñoz M, Rosso M, Robles-Frias MJ, Salinas-Martín MV, Rosso R, González-Ortega A, Coveñas R (2010) The NK-1 receptor is expressed in human melanoma and is involved in the antitumor action of the NK-1 receptor antagonist aprepitant on melanoma cell lines. Lab Investig 90(8):1259–1269 Harkins J, Roper M, Ham RG, Stewart JM (1978) Biosynthesis of substance P in cultured mouse neuroblastoma and rat glioma cells. Brain Res 147(2):405–409 Ma J, Yuan S, Cheng J, Kang S, Zhao W, Zhang J (2016) Substance P promotes the progression of endometrial adenocarcinoma. Int J Gynecol Cancer: Off J Int Gynecol Cancer Soc 26(5):845–850. https://doi.org/10.1097/igc.0000000000000683 Akazawa T, Kwatra SG, Goldsmith LE, Richardson MD, Cox EA, Sampson JH, Kwatra MM (2009) A constitutively active form of neurokinin 1 receptor and neurokinin 1 receptor-mediated apoptosis in glioblastomas. J Neurochem 109(4):1079–1086 Bigioni M, Benzo A, Irrissuto C, Maggi CA, Goso C (2005) Role of NK-1 and NK-2 tachykinin receptor antagonism on the growth of human breast carcinoma cell line MDA-MB-231. Anti-Cancer Drugs 16(10):1083–1089 Entschladen F, Lang K, Drell TL, Joseph J, Zaenker KS (2002) Neurotransmitters are regulators for the migration of tumor cells and leukocytes. Cancer Immunol Immunother 51(9):467–482 Guha S, Eibl G, Kisfalvi K, Fan RS, Burdick M, Reber H, Hines OJ, Strieter R, Rozengurt E (2005) Broad-spectrum G protein-coupled receptor antagonist, [D-Arg1,D-Trp5,7,9,Leu11]SP: a dual inhibitor of growth and angiogenesis in pancreatic cancer. Cancer Res 65(7):2738–2745. https://doi.org/10.1158/0008-5472.can-04-3197 Munoz M, Gonzalez-Ortega A, Covenas R (2012) The NK-1 receptor is expressed in human leukemia and is involved in the antitumor action of aprepitant and other NK-1 receptor antagonists on acute lymphoblastic leukemia cell lines. Investig New Drugs 30(2):529–540. https://doi.org/10.1007/s10637-010-9594-0 Munoz M, Rosso M, Perez A, Covenas R, Rosso R, Zamarriego C, Piruat JI (2005) The NK1 receptor is involved in the antitumoural action of L-733,060 and in the mitogenic action of substance P on neuroblastoma and glioma cell lines. Neuropeptides 39(4):427–432. https://doi.org/10.1016/j.npep.2005.03.004 Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F (2015) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136(5):E359–E386. https://doi.org/10.1002/ijc.29210 Arab M, Noghabaei G, Kazemi SN (2014) Comparison of crude and age-specific incidence rates of breast, ovary, endometrium and cervix cancers in Iran, 2005. Asian Pac J Cancer Prevent: APJCP 15(6):2461–2464 Plataniotis G, Castiglione M, Group EGW (2010) Endometrial cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol 21(suppl 5):v41–v45 Faria SC, Sagebiel T, Balachandran A, Devine C, Lal C, Bhosale PR (2015) Imaging in endometrial carcinoma. Indian J Radiol Imaging 25(2):137–147. https://doi.org/10.4103/0971-3026.155857 Reddy BY, Greco SJ, Patel PS, Trzaska KA, Rameshwar P (2009) RE-1–silencing transcription factor shows tumor-suppressor functions and negatively regulates the oncogenic TAC1 in breast cancer cells. Proc Natl Acad Sci. https://doi.org/10.1073/pnas.0809130106 Rosso M, Munoz M, Berger M (2012) The role of neurokinin-1 receptor in the microenvironment of inflammation and cancer. Sci World J. https://doi.org/10.1100/2012/381434 Simeonidis S, Castagliuolo I, Pan A, Liu J, Wang CC, Mykoniatis A, Pasha A, Valenick L, Sougioultzis S, Zhao D, Pothoulakis C (2003) Regulation of the NK-1 receptor gene expression in human macrophage cells via an NF-kappa B site on its promoter. Proc Natl Acad Sci USA 100(5):2957–2962. https://doi.org/10.1073/pnas.0530112100 Friess H, Zhu Z, Liard V, Shi X, Shrikhande SV, Wang L, Lieb K, Korc M, Palma C, Zimmermann A, Reubi JC, Buchler MW (2003) Neurokinin-1 receptor expression and its potential effects on tumor growth in human pancreatic cancer. Lab Investig: A J Tech Methods Pathol 83(5):731–742