Evaluation of plasma and red cells obtained after leucocyte depletion of whole blood

Transfusion Medicine - Tập 9 Số 1 - Trang 51-61 - 1999
Gary Williamson1,2, Rider3, Jonathan R. Swann2,4, ̇Winter3, Rawshan Ali2, D Pamphilon3
1Division of Transfusion Medicine, University of Cambridge, Cambridge CB2 2PT,
2National Blood Service, East Anglia Centre, Long Road, Cambridge CB2 2PT,
3Bristol Institute for Transfusion Sciences, National Blood Service, Southmead Road, Bristol BS10 5ND,
4Pall Medsep, Walton Road, Portsmouth PO6 1TD, UK

Tóm tắt

We evaluated whole blood integral filtration to produce leucocyte‐depleted red cells and plasma by using the WBF1 whole blood filter (Pall Medsep). Whole blood units were filtered after either warm (2–4 h at room temperature) or cold (12–24 h at 4 °C) holds. Filtered and control units were processed using either a bottom‐and‐top or top‐top method. Red cells were tested weekly for 6 weeks, and plasma 3 monthly for 12 months. All filtered red‐cell packs contained < 5 × 106 leucocytes/unit with 71 of 72 containing < 1 × 106 leucocytes/unit. No clinically significant differences in red‐cell storage parameters were seen, although haemolysis was less and pO2/pCO2 values were better maintained in filtered units. Plasma units contained < 2.5 × 103 leucocytes/unit with no significant loss of factor VIII except in the warm hold units processed by the top‐top method. There was no evidence of complement or coagulation activation with significant removal of preformed C3a in cold hold units. Plasma storage parameters were maintained at control levels for 12 months.

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Tài liệu tham khảo

10.1182/blood.V86.9.3598.bloodjournal8693598

10.1016/S0887-7963(93)70027-9

10.1111/j.1423-0410.1993.tb02124.x

Council of Europe(1997)Guide to the Use Preparation and Quality Control of Blood Components 3rd edn. Council of Europe Publishing.

UKBTS/NIBSC(1997)Guidelines for the Blood Transfusion Service in the United Kingdom 3rd edn. HMSO London.

10.1111/j.1365-2141.1994.tb04923.x

10.1056/NEJM199409083311001

10.1016/S0140-6736(96)06168-5

10.1097/00005373-199603000-00009

10.1111/j.1423-0410.1991.tb00907.x

10.1046/j.1537-2995.1996.36896374375.x

10.1093/ajcp/95.5.733

10.1016/0268-960X(95)90035-7

10.1046/j.1537-2995.1996.36596282594.x

10.1046/j.1423-0410.1997.7310028.x

10.1111/j.1423-0410.1994.tb00272.x

10.1046/j.1365-2141.1996.d01-1785.x

Rider J.R. Winter M.A. Payrat J.‐M. Mathias J.‐M. Pamphilon D.H.(in press)Leucocytes can be eliminated from plasma by filtration prior to viral inactivation with methylene blue.Vox Sanguinis.

10.1046/j.1537-2995.1997.371098016442.x

10.1111/j.1423-0410.1995.tb02594.x

Schleuning M., 1992, Complement activation during storage of blood under normal blood bank conditions. Effects of proteinase inhibitors and leukocyte depletion, Blood, 79, 3071, 10.1182/blood.V79.11.3071.3071

10.1046/j.1537-2995.1996.36496226145.x

10.1046/j.1537-2995.1996.36896374374.x

10.1046/j.1537-2995.1994.34194098597.x

Van De Watering L.M.G. Houbiers J. Hermans J. Harvey M. Bouter H. Boer F. Husymans H. Brand A.(1996) Leucocyte depletion reduces postoperative mortality in patients undergoing cardiac surgery.Abstract SY3DI‐06 24th Congress International Society of Blood Transfusion Japan.

Williamson L.M., 1994, Bedside filtration of blood products in the prevention of HLA alloimmunisation — a prospective randomised study, Blood, 83, 3028, 10.1182/blood.V83.10.3028.3028