Evaluation of in vitro cytotoxic activity of mono-PEGylated StAP3 (Solanum tuberosum aspartic protease 3) forms

Bell, 2003, Arming the enemy: the evolution of resistance to self-proteins, Microbiology, 149, 1367, 10.1099/mic.0.26265-0 Cowan, 1999, Plant products as antimicrobial agents, Clin. Microbiol. Rev., 12, 564, 10.1128/CMR.12.4.564 Hancock, 1999, Peptide antibiotics, Antimicrob. Agents Chemother., 43, 1317, 10.1128/AAC.43.6.1317 Hancock, 2000, The role of antimicrobial peptides in animal defenses, Proc. Natl. Acad. Sci. U. S. A., 97, 8856, 10.1073/pnas.97.16.8856 Levy, 2004, Antimicrobial proteins and peptides: anti-infective molecules of mammalian leukocytes, J. Leukoc. Biol., 76, 909, 10.1189/jlb.0604320 Reddy, 2004, Antimicrobial peptides: premises and promises, Int. J. Antimicrob. Agents, 24, 536, 10.1016/j.ijantimicag.2004.09.005 De Lucca, 2000, Antifungal peptides: potential candidates for the treatment of fungal infections, Expert Opin. Investig. Drugs, 9, 273, 10.1517/13543784.9.2.273 Chernysh, 2002, Antiviral and antitumor peptides from insects, Proc. Natl. Acad. Sci. U. S. A., 99, 12628, 10.1073/pnas.192301899 Chinchar, 2001, Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin, Virology, 288, 351, 10.1006/viro.2001.1080 Cruciani, 1991, Antibiotic magainins exert cytolytic activity against transformed cell lines through channel formation, Proc. Natl. Acad. Sci. U. S. A., 88, 3792, 10.1073/pnas.88.9.3792 Papo, 2005, Host defense peptides as new weapons in cancer treatment, Cell. Mol. Life Sci., 62, 784, 10.1007/s00018-005-4560-2 Zasloff, 2001, Antimicrobial peptides of multicellular organisms, Nature, 415, 389, 10.1038/415389a Zhang, 2001, Interaction of cationic antimicrobial peptides with model membranes, J. Biol. Chem., 276, 35714, 10.1074/jbc.M104925200 Ausubel, 2005, Are innate immune signaling pathways in plants and animals conserved?, Nat. Immunol., 6, 973, 10.1038/ni1253 Chisholm, 2006, Host–microbe interactions: shaping the evolution of the plant immune response, Cell, 124, 803, 10.1016/j.cell.2006.02.008 Dangl, 2001, Plant pathogens and integrated defence responses to infection, Nature, 411, 826, 10.1038/35081161 Van Loon, 1999, The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins, Physiol. Mol. Plant Pathol., 55, 85, 10.1006/pmpp.1999.0213 van Loon, 1970, Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. Samsun and Samsun NN. II. Changes in protein constitution after infection with tobacco mosaic virus, Virology, 40, 190, 10.1016/0042-6822(70)90395-8 Garcia-Olmedo, 2001, Antibiotic activities of peptides, hydrogen peroxide and peroxynitrite in plant defence, FEBS Lett., 498, 219, 10.1016/S0014-5793(01)02456-5 Arazi, 2002, Production of antiviral and antitumor proteins MAP30 and GAP31 in cucurbits using the plant virus vector ZYMV-AGII, Biochem. Biophys. Res. Commun., 292, 441, 10.1006/bbrc.2002.6653 Chu, 2006, Smilaxin, a novel protein with immunostimulatory, antiproliferative, and HIV-1-reverse transcriptase inhibitory activities from fresh Smilax glabra rhizomes, Biochem. Biophys. Res. Commun., 340, 118, 10.1016/j.bbrc.2005.12.010 González De Mejía, 2005, Lectins as bioactive plant proteins: a potential in cancer treatment, Crit. Rev. Food Sci. Nutr., 45 Lindholm, 2002, Cyclotides: a novel type of cytotoxic agents, Mol. Cancer Ther., 1, 365 Ngai, 2005, Phaseococcin, an antifungal protein with antiproliferative and anti-HIV-1 reverse transcriptase activities from small scarlet runner beans, Biochem. Cell Biol., 83, 212, 10.1139/o05-037 Wang, 2000, Anti-HIV and anti-tumor protein MAP30, a 30kDa single-strand type-I RIP, shares similar secondary structure and beta-sheet topology with the A chain of ricin, a type-II RIP, Protein Sci., 9, 138, 10.1110/ps.9.1.138 Guevara, 2002, An aspartic protease with antimicrobial activity is induced after infection and wounding in intercellular fluids of potato tubers, Eur. J. Plant Pathol., 108, 131, 10.1023/A:1015049629736 Guevara, 2005, Molecular cloning of a potato leaf cDNA encoding an aspartic protease (StAsp) and its expression after P. infestans infection, Plant Physiol. Biochem., 43, 882, 10.1016/j.plaphy.2005.07.004 Mendieta, 2006, Antimicrobial activity of potato aspartic proteases (StAPs) involves membrane permeabilization, Microbiology, 152, 2039, 10.1099/mic.0.28816-0 Muñoz, 2011, Cholesterol and membrane phospholipid compositions modulate the leakage capacity of the swaposin domain from a potato aspartic protease (StAsp-PSI), Biochim. Biophys. Acta, 1811, 1038, 10.1016/j.bbalip.2011.08.013 Muñoz, 2010, The swaposin-like domain of potato aspartic protease (StAsp-PSI) exerts antimicrobial activity on plant and human pathogens, Peptides, 31, 777, 10.1016/j.peptides.2010.02.001 Muñoz, 2014, Possible mechanism of structural transformations induced by StAsp-PSI in lipid membranes, Biochim. Biophys. Acta, 1838, 339, 10.1016/j.bbamem.2013.08.004 Katre, 1993, The conjugation of proteins with polyethylene glycol and other polymers: altering properties of proteins to enhance their therapeutic potential, Adv. Drug Deliv. Rev., 10, 91, 10.1016/0169-409X(93)90005-O Veronese, 1996, Improvement of pharmacokinetic, immunological and stability properties of asparaginase by conjugation to linear and branched monomethoxy poly(ethylene glycol), J. Control. Release, 40, 199, 10.1016/0168-3659(95)00185-9 Harris, 2003, Effect of PEGylation on pharmaceuticals, Nat. Rev. Drug Discov., 2, 214, 10.1038/nrd1033 Harris, 2001, PEGylation: a novel process for modifying pharmacokinetics, Clin. Pharmacokinet., 40, 539, 10.2165/00003088-200140070-00005 Roberts, 2002, Chemistry for peptide and protein PEGylation, Adv. Drug Deliv. Rev., 54, 459, 10.1016/S0169-409X(02)00022-4 Lee, 2003, Intranasal delivery of PEGylated salmon calcitonins: hypocalcemic effects in rats, Calcif. Tissue Int., 73, 545, 10.1007/s00223-002-0034-9 Lee, 1999, Preparation and characterization of polyethylene-glycol-modified salmon calcitonins, Pharm. Dev. Technol., 4, 269, 10.1081/PDT-100101361 Na, 2006, Effect of molecular size of PEGylated recombinant human epidermal growth factor on the biological activity and stability in rat wound tissue, Pharm. Dev. Technol., 11, 513, 10.1080/10837450600941053 Veronese, 2005, PEGylation, successful approach to drug delivery, Drug Discovery Today, 10, 1451, 10.1016/S1359-6446(05)03575-0 Gaertner, 1996, Site-specific attachment of functionalized poly(ethylene glycol) to the amino terminus of proteins, Bioconjug. Chem., 7, 38, 10.1021/bc950074d Greenwald, 2003, Effective drug delivery by PEGylated drug conjugates, Adv. Drug Deliv. Rev., 55, 217, 10.1016/S0169-409X(02)00180-1 Veronese, 2001, Peptide and protein PEGylation: a review of problems and solutions, Biomaterials, 22, 405, 10.1016/S0142-9612(00)00193-9 Whelan, 2005, Beyond PEGylation, Drug Discovery Today, 10, 301, 10.1016/S1359-6446(05)03387-8 Federico, 2006, Histaminase PEGylation: preparation and characterization of a new bioconjugate for therapeutic application, J. Control. Release, 115, 168, 10.1016/j.jconrel.2006.07.020 Li, 2009, Anti-tumor activity and immunological modification of ribosome-inactivating protein (RIP) from Momordica charantia by covalent attachment of polyethylene glycol, Acta Biochim. Biophys. Sin., 41, 792, 10.1093/abbs/gmp068 Meng, 2012, Preparation of an antitumor and antivirus agent: chemical modification of alpha-MMC and MAP30 from Momordica charantia L. with covalent conjugation of polyethylene glycol, Int. J. Nanomed., 7, 3133 Ueno, 1996, Polyethylene glycol-modified pokeweed mitogen (PWM) as a potential non-immunogenic stimulator of lymphokine-activated killer cells, J. Biomater. Sci. Polym. Ed., 7, 753, 10.1163/156856296X00093 Ikeda, 2005, Phenylalanine ammonia-lyase modified with polyethylene glycol: potential therapeutic agent for phenylketonuria, Amino Acids, 29, 283, 10.1007/s00726-005-0218-5 Matoušek, 2010, Plant ribonucleases and nucleases as antiproliferative agents targeting human tumors growing in mice, Recent Pat. DNA Gene Seq., 4, 29, 10.2174/187221510790410813 Matousek, 2009, Antitumor effects and cytotoxicity of recombinant plant nucleases, Oncol. Res., 18, 163, 10.3727/096504009790217425 Podaralla, 2012, Synthesis of novel biodegradable methoxy poly(ethylene glycol)-zein micelles for effective delivery of curcumin, Mol. Pharm., 9, 2778, 10.1021/mp2006455 Guevara, 2001, Purification and characterization of an aspartic protease from potato leaves, Physiol. Plant., 112, 321, 10.1034/j.1399-3054.2001.1120304.x Neuhoff, 1988, Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250, Electrophoresis, 9, 255, 10.1002/elps.1150090603 Bradford, 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 Kinstler, 1996, Characterization and stability of N-terminally PEGylated rhG-CSF, Pharm. Res., 13, 996, 10.1023/A:1016042220817 Kurfurst, 1992, Detection and molecular weight determination of polyethylene glycol-modified hirudin by staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Anal. Biochem., 200, 244, 10.1016/0003-2697(92)90460-O da Silva Freitas, 2010, Biochemical and biophysical characterization of lysozyme modified by PEGylation, Int. J. Pharm., 392, 111, 10.1016/j.ijpharm.2010.03.036 Bailon, 1998, PEG-conjugated pharmaceutical proteins, Pharm. Sci. Technol. Today, 1, 352, 10.1016/S1461-5347(98)00086-8 Caliceti, 2003, Pharmacokinetic and biodistribution properties of poly(ethylene glycol)-protein conjugates, Adv. Drug Deliv. Rev., 55, 1261, 10.1016/S0169-409X(03)00108-X Collen, 2000, Polyethylene glycol-derivatized cysteine-substitution variants of recombinant staphylokinase for single-bolus treatment of acute myocardial infarction, Circulation, 102, 1766, 10.1161/01.CIR.102.15.1766 Planas, 2002, Solid-phase synthesis of new peptide–arene hybrids from N-TCP amino acids, Tetrahedron Lett., 43, 4431, 10.1016/S0040-4039(02)00836-5 Thevissen, 1999, Permeabilization of fungal membranes by plant defensins inhibits fungal growth, Appl. Environ. Microbiol., 65, 5451, 10.1128/AEM.65.12.5451-5458.1999 Imura, 2007, Action mechanism of tachyplesin I and effects of PEGylation, Biochim. Biophys. Acta, 1768, 1160, 10.1016/j.bbamem.2007.01.005 Epand, 2009, Lipid domains in bacterial membranes and the action of antimicrobial agents, Biochim. Biophys. Acta, 1788, 289, 10.1016/j.bbamem.2008.08.023 Nikaido, 1989, Outer membrane barrier as a mechanism of antimicrobial resistance, Antimicrob. Agents Chemother., 33, 1831, 10.1128/AAC.33.11.1831 Guiotto, 2003, PEGylation of the antimicrobial peptide nisin A: problems and perspectives, Farmaco, 58, 45, 10.1016/S0014-827X(02)01301-0 Imura, 2007, Action mechanism of PEGylated magainin 2 analogue peptide, Biochim. Biophys. Acta, 1768, 2578, 10.1016/j.bbamem.2007.06.013 Wu, 2007, Impact of pro segments on the folding and function of human neutrophil alpha-defensins, J. Mol. Biol., 368, 537, 10.1016/j.jmb.2007.02.040 Asthana, 2004, Dissection of antibacterial and toxic activity of melittin: a leucine zipper motif plays a crucial role in determining its hemolytic activity but not antibacterial activity, J. Biol. Chem., 279, 55042, 10.1074/jbc.M408881200 Fox, 2012, Design and characterization of novel hybrid antimicrobial peptides based on cecropin A, LL-37 and magainin II, Peptides, 33, 197, 10.1016/j.peptides.2012.01.013 Kondejewski, 1996, Gramicidin S is active against both gram-positive and gram-negative bacteria, Int. J. Pept. Protein Res., 47, 460, 10.1111/j.1399-3011.1996.tb01096.x Ryan, 2006, Antimicrobial activity of native and synthetic surfactant protein B peptides, J. Immunol., 176, 416, 10.4049/jimmunol.176.1.416 Abraham, 2005, Isothermal titration calorimetry studies of the binding of the antimicrobial peptide gramicidin S to phospholipid bilayer membranes, Biochemistry, 44, 11279, 10.1021/bi050898a Verly, 2008, Effect of cholesterol on the interaction of the amphibian antimicrobial peptide DD K with liposomes, Peptides, 29, 15, 10.1016/j.peptides.2007.10.028 Yau, 1998, The preference of tryptophan for membrane interfaces, Biochemistry, 37, 14713, 10.1021/bi980809c Jedlovsky, 2003, Effect of cholesterol on the properties of phospholipid membranes. 1. Structural features, J. Phys. Chem. B, 5311, 10.1021/jp0219505 Mendieta, 2010, Cytotoxic effect of potato aspartic proteases (StAPs) on Jurkat T cells, Fitoterapia, 81, 329, 10.1016/j.fitote.2009.10.004