Evaluation of enrichment method for the detection of <i><scp>V</scp>ibrio cholerae </i><scp>O</scp>1 using a rapid dipstick test in <scp>B</scp>angladesh

Tropical Medicine and International Health - Tập 19 Số 3 - Trang 301-307 - 2014
Christine Marie George1, R. Bradley Sack2, David A. Sack1, R. Bradley Sack1, KM Saif‐Ur‐Rahman2, Andrew S. Azman3, Shirajum Monira2, Shahnawaz Ahmed2, Zillur Rahman2, Md. Toslim Mahmud2, Munshi Mustafiz2, Munirul Alam2
1Department of International Health Program in Global Disease Epidemiology and Control Johns Hopkins Bloomberg School of Public Health Baltimore MD USA
2International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh
3Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA

Tóm tắt

AbstractBackgroundTo evaluate the specificity of the Crystal VC dipstick test for detecting cholera.MethodsWe compared direct testing using the Crystal VC dipstick test and testing after enrichment for 6 h in alkaline peptone water (APW) to bacterial culture as the gold standard. Samples positive by dipstick but negative by culture were also tested using PCR.ResultsStool was collected from 125 patients. The overall specificities of the direct testing and testing after 6‐h enrichment in APW compared to bacterial culture were 91.8% and 98.4% (P = 0.125), respectively, and the sensitivities were 65.6% and 75.0% (P = 0.07), respectively.ConclusionThe increase in the sensitivity of the Crystal VC kit with the use of the 6‐h enrichment step in APW compared to direct testing was marginally significant. The Crystal VC dipstick had a much higher specificity than previously reported (91–98%). Therefore, this method might be a promising screening tool for cholera outbreak surveillance in resource‐limited settings where elimination of false‐positive results is critical.

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Tropical Medicine and International Health

Tập 19 Số 3

301-307

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