Evaluation of different culture techniques of osteoblasts on 3D scaffolds

Central European Journal of Biology - Tập 5 - Trang 456-465 - 2010
Ying-ying Wu1,2, Yu Ban1,2, Ning Geng3, Yong-yue Wang1, Xiao-guang Liu4, Tao Yu5,2, Ping Gong1
1Department of Implantology, West China College of Stomatology, Sichuan University, Chengdu, Sichuan, People’s Republic of China
2State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, People’s Republic of China
3Department of Pathology, West China College of Stomatology, Sichuan University, Chengdu, Sichuan, People’s Republic of China
4Department of Biomaterials Center, Sichuan University, Chengdu, Sichuan, People’s Republic of China
5Department of Oral & Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, Sichuan, People's Republic of China

Tóm tắt

Bones adjust their structure to withstand the mechanical demands they experience. It is suggested that flow-derived shear stress may be the most significant and primary mediator of mechanical stimulation. In this study, we designed and fabricated a fluid flow cell culture system that can load shear stress onto cells cultured on 3D scaffolds. We evaluated the effect of different culture techniques, namely, (1) continuous perfusion fluid flow, (2) intermittent perfusion fluid flow, and (3) static condition, on the proliferation of osteoblasts seeded on partially deproteinized bones. The flow rate was set at 1 ml/min for all the cells cultured using flow perfusion and the experiment was conducted for 12 days. Scanning electron microscopy analysis indicated an increase in cell proliferation for scaffolds subjected to fluid shear stress. In addition, the long axes of these cells lengthened along the flowing fluid direction. Continuous perfusion significantly enhanced cell proliferation compared to either intermittent perfusion or static condition. All the results demonstrated that fluid shear stress is able to enhance the proliferation of cells and change the form of cells.

Tài liệu tham khảo

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