Evaluation of autologous bone marrow in wound healing in animal model: a possible application of autologous stem cells

International Wound Journal - Tập 9 Số 5 - Trang 505-516 - 2012
Ashok Akela1, Samit Kumar Nandi2, Dibyajyoti Banerjee3, Partha Das4, Subhasis Roy5, Siddhartha Narayan Joardar6, Mohan Mandal7, Pradip Kumar Das8, Nilu Pradhan9
1Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India
2SK Nandi, PhD, Department of Veterinary Surgery & Radiology, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, West Bengal, India
3D Banerjee, MD, Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India
4P Das, PhD, Department of Veterinary Anatomy, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, West Bengal, India
5S Roy, PhD, Department of Veterinary Surgery & Radiology, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, West Bengal, India
6SN Joardar, PhD, Department of Veterinary Microbiology, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, West Bengal, India
7M Mandal, PhD, NRC on Mithun (ICAR), Jharnapani 797106, Nagaland, India
8PK Das, PhD, Department of Veterinary Physiology, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, West Bengal, India
9NR Pradhan, PhD, Department of Veterinary Medicine, West Bengal University of Animal & Fishery Sciences, Kolkata 700037, West Bengal, India

Tóm tắt

A study was conducted to evaluate the potential of autologous bone marrow‐derived cells in comparison with buffy coat of autologous blood for rapid cutaneous wound healing in rabbit model. Three square full‐thickness skin excisional wounds were created in 15 selected experimental animals (rabbit) divided randomly into three groups. The wound was treated with autologous bone marrow cells in plasma (group 1), buffy coat of blood in plasma (group 2) and autologous plasma as control (group 3). Wounds were observed for 30 days for granulation tissue formation, biochemical, histomorphological and histochemical evaluation. In this study, granulation tissue appeared significantly lesser in wounds of group 3 animals followed by group 2 and 1 animals. Neovascularisation, granulation tissue formation, denser, thicker and better arranged collagen fibres, reticulin fibres and elastin fibres formation was more in group 1 as compared with other groups. It was concluded that the application of bone marrow‐derived nucleated cells into the wound margins resulted in early and significantly faster rate of complete healing as compared with buffy coat of autologous blood and autologous plasma (control). This approach may be beneficial in various surface wounds that heal at a slower rate and recommended for healing of various complicated wound in future.

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International Wound Journal

Tập 9 Số 5

505-516

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