Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthiumL. ethanolic extracts

Springer Science and Business Media LLC - Tập 6 Số 1 - 2012
Oana Crăciunescu1, Daniel Constantin1, Alexandra Gaspar‐Pintiliescu1, L. D. Toma1, Elena Uţoiu1, Lucia Moldovan1
1Department of Cellular and Molecular Biology, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, 060031, Bucharest, Romania

Tóm tắt

AbstractBackground

Arnica montanaL. andArtemisia absinthiumL. (Asteraceae) are medicinal plants native to temperate regions of Europe, including Romania, traditionally used for treatment of skin wounds, bruises and contusions. In the present study,A. montanaandA. absinthiumethanolic extracts were evaluated for their chemical composition, antioxidant activity and protective effect against H2O2-induced oxidative stress in a mouse fibroblast-like NCTC cell line.

Results

A. absinthiumextract showed a higher antioxidant capacity thanA. montanaextract as Trolox equivalent antioxidant capacity, Oxygen radical absorbance capacity and 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging activity, in correlation with its flavonoids and phenolic acids content. Both plant extracts had significant effects on the growth of NCTC cells in the range of 10–100 mg/LA. montanaand 10–500 mg/LA. absinthium. They also protected fibroblast cells against hydrogen peroxide-induced oxidative damage, at the same doses. The best protection was observed in cell pre-treatment with 10 mg/LA. montanaand 10–300 mg/LA. absinthium, respectively, as determined by Neutral red and lactate dehydrogenase assays. In addition, cell pre-treatment with plant extracts, at these concentrations, prevented morphological changes induced by hydrogen peroxide. Flow-cytometry analysis showed that pre-treatment withA. montanaandA. absinthiumextracts restored the proportion of cells in each phase of the cell cycle.

Conclusions

A. montanaandA. absinthiumextracts, rich in flavonoids and phenolic acids, showed a good antioxidant activity and cytoprotective effect against oxidative damage in fibroblast-like cells. These results provide scientific support for the traditional use ofA. montanaandA. absinthiumin treatment of skin disorders.

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Tài liệu tham khảo

Migdal C, Serres M: Reactive oxygen species and oxidative stress. Med Sci (Paris). 2011, 27: 405-412. 10.1051/medsci/2011274017.

Avery SV: Molecular targets of oxidative stress. Biochem J. 2011, 434: 201-210. 10.1042/BJ20101695.

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J: Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007, 39: 44-84. 10.1016/j.biocel.2006.07.001.

Buyukokuroglu ME, Gulcin I, Oktay M, Kufrevioglu OI: In vitro antioxidant properties of dantrolene sodium. Pharmacol Res. 2001, 44: 491-494. 10.1006/phrs.2001.0890.

Mensah AY, Sampson J, Houghton PJ, Hylands PJ, Westbrook J, Dunn M, Hughes MA, Cherry GW: Effects of Buddleja globosa leaf and its constituents relevant to wound healing. J Ethnopharmacol. 2001, 77: 219-226. 10.1016/S0378-8741(01)00297-5.

Gulcin I, Kufrevioglu OI, Oktay M, Buyukokuroglu ME: Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioicaL.). J Ethnopharmacol. 2004, 90: 205-215. 10.1016/j.jep.2003.09.028.

Pinnell SR: Cutaneous photodamage, oxidative stress, and topical antioxidant protection. J Am Acad Dermatol. 2003, 48: 1-19. 10.1067/mjd.2003.16.

Svobodova A, Walterova D, Psotova J: Influence of silymarin and its flavonolignans on H2O2-induced oxidative stress in human keratinocytes and mouse fibroblasts. Burns. 2006, 32: 973-979. 10.1016/j.burns.2006.04.004.

Houghton PJ, Hylands PJ, Mensah AY, Deters AM: In vitro tests and ethnopharmacological investigations: wound healing as an example. J Ethnopharmacol. 2005, 100: 100-107. 10.1016/j.jep.2005.07.001.

Maharwal J, Samarth RM, Saini MR: Radiomodulatory influence of Rajgira (Amaranthus paniculatus) leaf extract in Swiss albino mice. Phytother Res. 2003, 17: 1150-1154. 10.1002/ptr.1340.

Katiyar SK, Afaq F, Perez A, Mukhtar H: Green tea polyphenol (3)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress. Carcinogenesis. 2001, 22: 287-294. 10.1093/carcin/22.2.287.

Ginsburg I, Kohen R, Koren E: Microbial and host cells acquire enhanced oxidant-scavenging abilities by binding polyphenols. Arch Biochem Biophys. 2011, 506: 12-23. 10.1016/j.abb.2010.11.009.

Auld CA, Hopkins RG, Fernandes KM, Morrison RF: Novel effect of helenalin on Akt signaling and Skp2 expression in 3 T3-L1 preadipocytes. Biochem Biophys Res Commun. 2006, 346: 314-320. 10.1016/j.bbrc.2006.05.117.

Klaas CA, Wagner G, Laufer S, Sosa S, Loggia R, Bomme U, Pahl HL, Merfort I: Studies on the anti-inflammatory activity of phytopharmaceuticals prepared from Arnica flowers. Planta Med. 2002, 68: 385-391. 10.1055/s-2002-32067.

Shahidi F, Naczk M: Antioxidant Properties of Food Phenolics. In Phenolics in food and nutraceuticals 1. 2004, Boca Raton: CRC Press, 397-438.

Alexandru V, Balan M, Gaspar A, Craciunescu O, Moldovan L: Studies on the antioxidant activity, phenol and flavonoid contents of some selected Romanian medicinal plants used for wound healing. Rom Biotechnol Lett. 2007, 12: 3467-3472.

Craciunescu O, Stefan LM, Balan M, Moldovan L: Evaluation of the radioprotective activity of new green tea-collagen materials. J Optoelectron Adv M. 2007, 9: 2602-2607.

Du G, Li M, Ma F, Liang D: Antioxidant capacity and the relationship with polyphenol and vitamin C in Actinidia fruits. Food Chem. 2009, 113: 557-562. 10.1016/j.foodchem.2008.08.025.

Ou B, Hampsch-Woodill M, Prior RL: Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. J Agric Food Chem. 2001, 49: 4916-4926.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999, 26: 1231-1237. 10.1016/S0891-5849(98)00315-3.

Huang D, Ou B, Prior RL: The chemistry behind antioxidant capacity assays. J Agr Food Chem. 2005, 53: 1841-1856. 10.1021/jf030723c.

Moldovan L, Gaspar A, Toma L, Craciunescu O, Saviuc C: Comparison of polyphenolic content and antioxidant capacity of five Romanian traditional medicinal plants. Rev Chim-Bucharest. 2011, 62: 299-303.

Canadanovic-Brunet JM, Djilas SM, Cetkovic GS, Tumbas VT: Free-radical scavenging activity of wormwood (Artemisia absinthiumL.) extracts. J Sci Food Agri. 2005, 85: 265-272. 10.1002/jsfa.1950.

Kondo K, Kurihara M, Fukuhara K: Mechanism of antioxidant effects of catechins. Methods in Enzymology. Edited by: Packer L. 2001, San Diego: Academic Press, 203-216.

van Acker SA, van den Berg DJ, Tromp MN, Griffioen DH, van Bennekom WP, van der Vijgh WJ, Bast A: Structural aspects of antioxidant activity of flavonoids. Free Radic Biol Med. 1996, 20: 331-432. 10.1016/0891-5849(95)02047-0.

Kouzi SA, Nuzum DS: Arnica for bruising and swelling. Am J Health Syst Pharm. 2007, 64: 2434-2443. 10.2146/ajhp070155.

Ozgen U, Mavi A, Terzi Z, Coskun M, Yildirim A: Antioxidant activities and total phenolic compounds amount of some Asteraceae species. Turkish J Pharm Sci. 2004, 1: 203-216.

Pan W, Dancik CM, Nelson VM, Jiang ZG, Lebowitz MS, Ghanbari HA: A novel neuroprotectant PAN-811 protects neurons from oxidative stress. Cent Eur J Biol. 2009, 4: 34-40. 10.2478/s11535-008-0062-1.

Lee SM, Youn B, Kim CS, Kim CS, Kang C, Kim J: Gamma-irradiation and doxorubicin treatment of normal human cells cause cell cycle arrest via different pathways. Mol Cells. 2005, 20: 331-338.

Baran I, Ganea C, Scordino A, Musumeci F, Barresi V, Tudisco S, Privitera S, Grasso R, Condorelli DF, Ursu I, Baran V, Katona E, Mocanu MM, Gulino M, Ungureanu R, Surcel M, Ursaciuc C: Effects of menadione, hydrogen peroxide and quercetin on apoptosis and delayed luminiscence of human leukemia Jurkat T-cells. Cell Biochem Biophys. 2010, 58: 169-179. 10.1007/s12013-010-9104-1.

von Bruggisser R, Daeniken K, Jundt G, Schaffner W, Tullberg-Reinert H: Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay. Planta Med. 2002, 68: 445-448. 10.1055/s-2002-32073.

Mao QQ, Xian YF, Ip SP, Tsai SH, Che CT: Protective effects of peony glycosides against corticosterone-induced cell death in PC12 cells through antioxidant action. J Ethnopharmacol. 2011, 133: 1121-1125. 10.1016/j.jep.2010.11.043.

Park KI, Park HS, Kang SR, Nagappan A, Lee DH, Kim JA, Han DY, Kim GS: Korean Scutellaria baicalensis water extract inhibits cell cycle G1/S transition by suppressing cyclin D1 expression and matrix metalloproteinase-2 activity in human lung cancer cells. J Ethnopharmacol. 2011, 133: 634-641. 10.1016/j.jep.2010.10.057.

Seomun Y, Kim JT, Kim HS, Park JY, Joo CK: Induction of p21Cip1-mediated G2/M arrest in H2O2-treated lens epithelial cells. Molecular Vision. 2005, 11: 764-774.

Koczor CA, Shokolenko IN, Boyd AK, Balk SP, Wilson GL, Ledoux SP: Mitochondrial DNA damage initiates a cell cycle arrest by a Chk2-associated mechanism in mammalian cells. J Biol Chem. 2009, 2284: 36191-36201.

Singleton VL, Orthofer R, Lamuela-Raventos RM: Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology. Edited by: Abelson JN, Simon MI. 1999, San Diego: Academic Press, 152-177.

Ebrahimzadeh MA, Nabavi SF, Nabavi SM, Eslami B: Antihemolytic and antioxidant activities of Allium paradoxum. Cent Eur J Biol. 2010, 5: 338-345. 10.2478/s11535-010-0013-5.

Fotakis G, Timbrell JA: In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett. 2006, 160: 171-177. 10.1016/j.toxlet.2005.07.001.

Saad B, Abu-Hijleh G, Suter UW: Polymer biocompatibility assessment by cell culture techniques. Introduction to Polymeric Biomaterials 1. Edited by: Arshady R. 2003, Australia: Citus Books

Ge P, Zhang J, Wang X, Meng F, Li W, Luan Y, Ling F, Luo Y: Inhibition of autophagy induced by proteasome inhibition increases cell death in human SHG-44 glioma cells. Acta Pharmacol Sin. 2009, 30: 1046-1052. 10.1038/aps.2009.71.

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