Evaluation of anti-adipogenic active homoisoflavonoids from Portulaca oleracea

Zeitschrift fur Naturforschung - Section C Journal of Biosciences - Tập 74 Số 9-10 - Trang 265-273 - 2019
Jung Im Lee1, Jung Hwan Oh2, Chang‐Suk Kong2,1, Youngwan Seo3,4
1Marine Biotechnology Center for Pharmaceuticals and Foods, College of Medical and Life Sciences, Silla University , Busan 46958 , Republic of Korea
2Department of Food and Nutrition, College of Medical and Life Sciences , Silla University , Busan 46958 , Republic of Korea
3Department of Convergence Study on the Ocean Science and Technology, Ocean Science and Technology School, Korea Maritime and Ocean University, Busan 49112, Republic of Korea
4Division of Marine Bioscience, College of Ocean Science and Technology , Korea Maritime and Ocean University , Busan 49112 , Republic of Korea , Phone: +82-51-410-4328, Fax: +82-51-404-3538

Tóm tắt

Abstract

This study was performed to isolate antiobesity components from the crude extract of Portulaca oleracea. The crude extract was partitioned into n-hexane, 85% aqueous methanol, n-butanol, and water fractions. Their effects on adipogenic differentiation were evaluated in 3T3-L1 cells. Among the solvent fractions from P. olearacea, the 85% aq. MeOH effectively reduced the levels of lipid accumulation. Further purification of 85% aq. MeOH led to the isolation of the known homoisoflavonoids 1–4, as the active substances. The administration of homoisoflavonoids to adipocyte cells decreased the lipid accumulation and glucose consumption and increased the release of glycerol into culture medium. In particular, homoisoflavonoid 3 effectively down-regulated the adipogenic transcription genes such as peroxisome proliferator activated receptor-γ (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPα), and adipogenic target genes such as fatty acid binding protein 4 (FABP4), fatty acid transport protein 1 (FATP1), and acyl-CoA synthase 1 (ACS1).

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Zeitschrift fur Naturforschung - Section C Journal of Biosciences

Tập 74 Số 9-10

265-273

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