Evaluation of a Novel Precision Biotic on Enterohepatic Health Markers and Growth Performance of Broiler Chickens under Enteric Challenge

Animals - Tập 12 Số 19 - Trang 2502
Britt Blokker1, Cristiano Bortoluzzi1, Christelle Iaconis2, Estefanía Pérez Calvo1, M. C. Walsh1, Ghislain Schyns1, Ian Tamburini1, Jack Geremia1
1DSM Nutritional Products, 4303 Kaiseraugst, Switzerland
2DSM Nutritional Products, 68128 Village-Neuf, France

Tóm tắt

This study evaluated the supplementation of a precision biotic (PB) on the enterohepatic health markers and growth performance of broiler chickens undergoing an enteric challenge. In the first study, three treatments were used: Unchallenged Control (UC); Challenged Control (CC; dietary challenge and 10× dose of coccidia vaccine); and a challenged group supplemented with PB (1.3 kg/ton). In the second study, three treatments were used: control diet, diet supplemented with Avilamycin (10 ppm), and a diet supplemented with PB (0.9 kg/ton). All the birds were exposed to natural challenge composed by dietary formulation and reused litter from a coccidiosis positive flock. In Trial 1, PB decreased ileal histological damage, increased villi length, and the expression of SLC5A8 in ileal tissue versus CC; it reduced ileal expression of IL-1β compared to both UC and CC treatments. PB increased the expression of cell cycling gene markers CCNA2 and CDK2 in the ileum compared to CC. In Trial 2, PB improved the growth performance, intestinal lesion scores and intestinal morphology of broiler chickens. These results indicate that birds supplemented with PB are more resilient to enteric challenges, probably by its action in modulating microbiome metabolic pathways related to nitrogen metabolism and protein utilization.

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Tài liệu tham khảo

Qin, 2010, A human gut microbial gene catalogue established by metagenomic sequencing, Nature, 464, 59, 10.1038/nature08821

Sergeant, M.J., Constantinidou, C., Cogan, T.A., Bedford, M.R., Penn, C.W., and Pallen, M.J. (2014). Extensive microbial and functional diversity within the chicken cecal microbiome. PLoS ONE, 9.

Glendinning, 2020, Assembly of hundreds of novel bacterial genomes from the chicken caecum, Genome Biol., 21, 34, 10.1186/s13059-020-1947-1

Walsh, 2021, A novel microbiome metabolic modulator improves the growth performance of broiler chickens in multiple trials and modulates targeted energy and amino acid metabolic pathways in the caecal metagenome, Poult. Sci., 100, 100800, 10.1016/j.psj.2020.10.054

Jacquier, V., Walsh, M.C., Schyns, G., Claypool, J., Blokker, B., Bortoluzzi, C., and Geremia, J. (2022). Evaluation of a Precision Biotic on the Growth Performance, Welfare Indicators, Ammonia Output, and Litter Quality of Broiler Chickens. Animals, 12.

Jiang, 2016, Metatranscriptomic analysis of diverse microbial communities reveals core metabolic pathways and microbiome-specific functionality, Microbiome, 4, 2, 10.1186/s40168-015-0146-x

Kers, 2018, Host and environmental factors affecting the intestinal microbiota in chickens, Front. Microbiol., 9, 235, 10.3389/fmicb.2018.00235

Geremia, J.M., Murphy, A.V., Han, S., Seigal, B.A., Landry, A., Sherry, K., Panos, S., Churchman, D., and O’Connor, A. (2016). Oligosaccharide Compositions and Methods for Producing Thereof. (Patent No. WO2016007778A1).

Geremia, J.M., Hoeller, U., Tamburini, I., Baur, M., Liobomirov, A.V., Canet-Martinez, E., Matthew Liu, C., Laprade, L.A., Schyns, G., and Hecht, M.B. (2020). Oligosaccharide Preparations and Compositions. (Patent No. WO2020097458).

Flint, 2008, Polysaccharide utilization by gut bacteria: Potential for new insights from genomic analysis, Nat. Rev. Microbiol., 6, 121, 10.1038/nrmicro1817

Koropatkin, 2012, How glycan metabolism shapes the human gut microbiota, Nat. Rev. Microbiol., 10, 323, 10.1038/nrmicro2746

Pourabedin, 2015, Prebiotics and gut microbiota in chickens, FEMS Microbiol. Lett., 362, fnv122, 10.1093/femsle/fnv122

Postler, 2017, Understanding the holobiont: How microbial metabolites affect human health and shape the immune system, Cell Metab., 26, 110, 10.1016/j.cmet.2017.05.008

Bordenstein, S.R., and Theis, K.R. (2015). Host biology in light of the microbiome: Ten principles of holobionts and hologenomes. PLoS Biol., 13.

Palliyeguru, 2010, Effect of dietary protein concentrates on the incidence of subclinical necrotic enteritis and growth performance of broiler chickens, Poult. Sci., 89, 1, 10.3382/ps.2009-00105

Livak, 2001, Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method, Methods, 25, 402, 10.1006/meth.2001.1262

Rueden, C.T., Schindelin, J., Hiner, M.C., DeZonia, B.E., Walter, A.E., Arena, E.T., and Eliceiri, K.W. (2017). ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinform., 18.

Takahashi, 2014, Histopathology of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis, World J. Gastroenterol., 20, 15539, 10.3748/wjg.v20.i42.15539

Chen, 2015, Identification of potential biomarkers for gut barrier failure in broiler chickens, Front. Vet. Sci., 2, 14, 10.3389/fvets.2015.00014

Tan, 2014, Supplemental dietary L-arginine attenuates intestinal mucosal disruption during a coccidial vaccine challenge in broiler chickens, Br. J. Nut., 112, 1098, 10.1017/S0007114514001846

Collier, 2008, Coccidia-induced mucogenesis promotes the onset of necrotic enteritis by supporting Clostridium perfringens growth, Vet. Immunol Immunop., 122, 104, 10.1016/j.vetimm.2007.10.014

Hamer, 2008, The role of butyrate on colonic function, Aliment. Pharm. Ther., 27, 104, 10.1111/j.1365-2036.2007.03562.x

Fachi, 2016, Regulation of immune cell function by short-chain fatty acids, Clin. Transl. Immunol., 5, e73, 10.1038/cti.2016.17

Kogut, 2022, Butyrate and intestinal homeostasis: Effects on the intestinal microbiota and epithelial hypoxia, Gut Microbiota, Immunity, and Health in Production Animals, Volume 4, 57, 10.1007/978-3-030-90303-9_4

Miska, 2017, The mRNA expression of amino acid and sugar transporters, aminopeptidase, as well as the di- and tri-peptide transporter PepT1 in the intestines of Eimeria infected broiler chickens, Poult. Sci., 96, 2, 10.3382/ps/pew303

Adedokun, 2012, Ileal endogenous amino acid losses: Response of broiler chickens to fiber and mild coccidial vaccine challenge, Poult. Sci., 91, 4, 10.3382/ps.2011-01777

Fournier, 2000, Alpha-1-acid glycoprotein, Biochim. Biophys. Acta, 1482, 157, 10.1016/S0167-4838(00)00153-9

Takahashi, 1998, Changes in plasma alpha 1-acid glycoprotein concentration and selected immune response in broiler chickens injected with Escherichia coli lipopolysaccharide, Br. Poult. Sci., 39, 152, 10.1080/00071669889547

Lee, 2012, Effects of Various Field Coccidiosis Control Programs on Host Innate and Adaptive Immunity in Commercial Broiler Chickens, Korean J. Poult. Sci., 39, 17, 10.5536/KJPS.2012.39.1.017

Kim, 2019, Involvement of T Cell Immunity in Avian Coccidiosis, Front. Immunol., 10, 2732, 10.3389/fimmu.2019.02732

Gibson, 2014, The chicken IL-1 family: Evolution in the context of the studied vertebrate lineage, Immunogenetics, 66, 427, 10.1007/s00251-014-0780-7

Li, R.W., and Li, C. (2006). Butyrate induces profound changes in gene expression related to multiple signal pathways in bovine kidney epithelial cells. BMC Genom., 7.

Li, 2008, Expression profiling of the solute carrier gene family in chicken intestine from the late embryonic to early post-hatch stages, Anim. Genet., 39, 407, 10.1111/j.1365-2052.2008.01744.x

Yin, 2010, Exposure of different bacterial inocula to newborn chicken affects gut microbiota development and ileum gene expression, ISME J., 4, 367, 10.1038/ismej.2009.128

Richards, 2008, Expression of proglucagon and proglucagon-derived peptide hormone receptor genes in the chicken, Gen. Comp. Endocrinol., 156, 323, 10.1016/j.ygcen.2008.01.014

Richards, 2009, The avian proglucagon system, Gen. Comp. Endocrinol., 163, 39, 10.1016/j.ygcen.2008.09.010

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Animals

Tập 12 Số 19

2502

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