Evaluation of Protein Adsorption on Atmospheric Plasma Deposited Coatings Exhibiting Superhydrophilic to Superhydrophobic Properties
Tóm tắt
Protein adsorption is one of the key parameters influencing the biocompatibility of medical device materials. This study investigates serum protein adsorption and bacterial attachment on polymer coatings deposited using an atmospheric pressure plasma jet system. The adsorption of bovine serum albumin and bovine fibrinogen (Fg) onto siloxane and fluorinated siloxane elastomeric coatings that exhibit water contact angles (θ) ranging from superhydrophilic (θ < 5°) to superhydrophobic (θ > 150°) were investigated. Protein interactions were evaluated in situ under dynamic flow conditions by spectroscopic ellipsometry. Superhydrophilic coatings showed lower levels of protein adsorption when compared with hydrophobic siloxane coatings, where preferential adsorption was shown to occur. Reduced levels of protein adsorption were also observed on fluorinated siloxane copolymer coatings exhibiting hydrophobic wetting behaviour. The lower levels of protein adsorption observed on these surfaces indicated that the presence of fluorocarbon groups have the effect of reducing surface affinity for protein attachment. Analysis of superhydrophobic siloxane and fluorosiloxane surfaces showed minimal indication of protein adsorption. This was confirmed by bacterial attachment studies using a Staphylococcus aureus strain known to bind specifically to Fg, which showed almost no attachment to the superhydrophobic coating after protein adsorption experiments. These results showed the superhydrophobic surfaces to exhibit antimicrobial properties and significantly reduce protein adsorption.
Tài liệu tham khảo
Ratner BD, Castner DG (1997) Surface modification of polymeric biomaterials. Springer, New York
Katsikogianni M, Misirlis Y (2004) Eur Cell Mater 8:37–57
Harold AS (2004) Biophys Chem 112(2–3):117–130
McClellan SJ, Franses EI (2005) Colloids Surf A 260(1–3):265–275
Zhang F, Kang ET, Neoh KG, Wang P, Tan KL (2001) Biomaterials 22(12):1541–1548
Chu PK, Chen J, Wang L, Huang N (2002) Mater Sci Eng R Rep 36(5–6):143–206
Favia P, d’Agostino R (1998) Surf Coat Technol 98(1–3):1102–1106
Schmidt DR, Waldeck H, Kao WJ (2009) Biol Interact Mater Surf, 1–18
Arima Y, Iwata H (2007) Biomaterials 28(20):3074–3082
Xie H-G, Li X-X, Lv G-J, Xie W-Y, Zhu J, Luxbacher T et al (2010) J. Biomed Mater Res Part A 92A(4):1357–1365
Sigal GB, Mrksich M, Whitesides GM (1998) J Am Chem Soc 120(14):3464–3473
Xu L-C, Siedlecki CA (2007) Biomaterials 28(22):3273–3283
Lee JH, Lee HB (1993) J Biomater Sci Polym Edition 4(5):467–481
Malmsten M (1995) Colloids Surf B 3(5):297–308
Nygren H, Alaeddin S, Lundström I, Magnusson K-E (1994) Biophys Chem 49(3):263–272
Ishizaki T, Saito N, Takai O (2010) Langmuir 26(11):8147–8154
Tamada Y, Ikada Y (1993) J Colloid Interface Sci 155(2):334–339
Yasuda T, Ishizuka K, Ezoe M (2008) IEEJ Trans Electr Electron Eng 3(3):290–296
Huang S-L, Ou C-F, Lai J-Y (1999) J Membr Sci 161(1–2):21–29
Roach P, Farrar D, Perry CC (2005) J Am Chem Soc 127(22):8168–8173
Goyal DK, Subramanian A (2010) Thin Solid Films 518(8):2186–2193
Berlind T, Tengvall P, Hultman L, Arwin H (2011) Acta Biomaterialia 7(3):1369–1378
Boss J (2000) Biomaterials and bioengineering handbook. Marcel Dekker, Inc, New York, pp 1–94
Slayter H (1983) Ann N Y Acad Sci 408(1):131–145
Foster T, Höök M (2007) Infections associated with indwelling medical devices. ASM Press, Herndon, pp 27–39
Albaugh J, O’Sullivan C, O’Neill L (2008) Surf Coat Technol 203(5–7):844–847
Feijter JAD, Benjamins J, Veer FA (1978) Biopolymers 17(7):1759–1772
Stenberg M, Nygren H (1983) Le Journal de Physique Colloques 44(C10):83–86
Stallard C, McDonnell K, Donegan M, Dowling DP (2010) Evaluation of spectroscopic ellipsometry for the measurement of BSA protein adhesion on atmospheric plasma modified surfaces. ECNF conference March 22–25, Liege, Belgium
Horsburgh MJ, Aish JL, White IJ, Shaw L, Lithgow JK, Foster SJ (2002) J Bacteriol 184(19):5457
O’Neill E, Pozzi C, Houston P, Humphreys H, Robinson DA, Loughman A et al (2008) J Bacteriol 190(11):3835
Houston P, Rowe SE, Pozzi C, Waters EM, O’Gara JP (2011) Infect Immun 79(3):1153
O’Neill L, Herbert PAF, Stallard C, Dowling DP (2010) Plasma Process Polym 7(1):43–50
Ramamoorthy A, Rahman M, Mooney DA, Don MacElroy JM, Dowling DP (2009) Plasma Process Polym 6(S1):S530–S536
Onda T, Shibuichi S, Satoh N, Tsujii K (1996) Langmuir 12(9):2125–2127
Schmidt-Szalowski K (2000) Plasmas Polym 5(3):173–190
Yang S-H, Liu C-H, Su C-H, Chen H (2009) Thin Solid Films 517(17):5284–5287
Seitz R, Brings R, Geiger R (2005) Appl Surf Sci 252(1):154–157
Tompkins HG, McGahan WA (1999) Spectroscopic ellipsometry and reflectometry. Wiley, New York
Lok BK, Cheng Y-L, Robertson CR (1983) J Colloid Interface Sci 91(1):104–116
Yaseen M, Salacinski H, Seifalian A, Lu J (2008) Biomed Mater 3:034123
Kumar V, Pulpytel J, Rauscher H, Mannelli I, Rossi F, Arefi-Khonsari F (2010) Fluorocarbon coatings via plasma enhanced chemical vapor deposition of 1H,1H,2H,2H-perfluorodecyl Acrylate-2, morphology, wettability and antifouling characterization. WILEY-VCH Verlag, pp 926–938
Koc Y, de Mello AJ, McHale G, Newton MI, Roach P, Shirtcliffe NJ (2008) Lab Chip 8(4):582–586
Khorasani MT, Mirzadeh H (2004) In vitro blood compatibility of modified PDMS surfaces as superhydrophobic and superhydrophilic materials. Wiley Subscription Services, Inc., A Wiley Company, New York, pp 2042–2047
Crick CR, Ismail S, Pratten J, Parkin IP (2011) Thin Solid Films 519(11):3722–3727
O’Gara JP (2007) FEMS Microbiol Lett 270(2):179–188