Evaluation of Antioxidant and Antimicrobial Activities and Phenolic Profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys

Springer Science and Business Media LLC - Tập 19 Số 5 - Trang 5490-5507
Laurian Vlase1, Daniela Benedec2, Daniela Hanganu3, Grigore Damian4, Ioan Csillag5, Bogdan Sevastre6, Augustin C. Moț7, Radu Silaghi‐Dumitrescu7, Ioan Ţilea8
1Department of Pharmaceutical Technology and Biopharmaceutics, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania.
2Department of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania.
3Department of Pharmacognosy, Iuliu Hatieganu University of Medicine and Pharmacy, 12 I. Creanga Street, Cluj-Napoca 400010, Romania. [email protected].
4Department of Physics, Babes-Bolyai University, 1 M. Kogalniceanu Street, Cluj-Napoca 400084, Romania.
5Department of Physics, Babes-Bolyai University, 1 M. Kogalniceanu Street, Cluj-Napoca 400084, Romania. [email protected].
6Department of Physiopathology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, Cluj-Napoca 400372, Romania
7Department of Chemistry and Chemical Engineering Babes-Bolyai University, 11 A. Janos Street, Cluj-Napoca 400028, Romania.
8Family Medicine, Department M3 Clinical Sciences Internal Medicine, University of Medicine and Pharmacy, 38 G. Marinescu Street, Târgu Mures 540139, Romania

Tóm tắt

This study was designed to examine the in vitro antioxidant and antimicrobial activities and to characterize the polyphenolic composition of the ethanolic extracts of Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys. Qualitative and quantitative analysis of the major phenolic compounds were conducted using high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). The total polyphenols, caffeic acid derivatives and flavonoids content was spectrophotometrically determined. The phenolic profile showed the presence of phenolic acid derivatives (caftaric, gentisic, caffeic, p-coumaric, chlorogenic and ferulic acids), flavonoid glycosides (rutin, isoquercitrin and quercitrin) and free flavonoid aglycons (luteolin, quercetin), in different concentrations. DPPH radical scavenging assay, Trolox equivalent antioxidant capacity (TEAC) method, hemoglobin ascorbate peroxidase activity inhibition (HAPX) assay, and electron paramagnetic resonance (EPR) radicals detection were employed, revealing several aspects of the antioxidant activities of these species. The antimicrobial tests were performed using the disk diffusion assay. These extracts contained a large amount of the polyphenolic compounds (77.72, 175.57, and 243.65 mg/g, respectively), and they showed a good antioxidant activity, as witnessed by a number of methods. T. chamaedrys had a high antimicrobial activity. Besides their antioxidant activity, the antimicrobial effect of these extracts confirms the biological activities of these herbal medicinal products.

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Springer Science and Business Media LLC

Tập 19 Số 5

5490-5507

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