Evaluation of Antimicrobial Property of Bacteriocin Capped Silver Nanoparticles Prepared from Cell Free Supernatant of Lactobacillus brevis Isolated from Marine Fishes
Tóm tắt
Drug resistance by microbes is becoming a major concern in the field of medicine. The oligo-dynamic nature of silver nanoparticles (AgNPs) is capable of killing even the multi-drug resistant organisms. In the current study, AgNPs were synthesized from cell free supernatant of Lactic Acid Bacteria (LAB), isolated from the gut of marine fishes viz., Lutjanus campechanus, Dendrobranchiata and Gerres subfasciatus. The synthesis of AgNPs was evaluated by the change in colour from pale-yellow to dark brown. Further, characterization based on Scanning Electron Microscopy and particle size analysis revealed the structure alignment, shape and average size of the synthesized AgNPs. X-Ray Diffraction revealed the unique diffraction peaks of the synthesized AgNPs. Fourier Transform Infra-Red spectroscopy confirmed the presence of carboxyl, amine and hydroxyl groups. The activity of the synthesized AgNPs was evaluated against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. Results revealed a substantial increase in activity against the tested bacteria. Further, successful binding of AgNPs with collagen asserts its application as a potential material with biological utility.
Tài liệu tham khảo
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