Evaluating the effectiveness of isolation distances for field plots of oilseed rape (Brassica napus) using a herbicide‐resistance transgene as a selectable marker

Plant Breeding - Tập 114 Số 4 - Trang 317-321 - 1995
Jodi Scheffler1, Russell Parkinson2, Phillip J. Dale2
1Institut für Allgemeine Botanik, Universityät Hamburg, Ohnhorststr. 18, D-22609 Hamburg, Germany
2John Innes Centre, Colney Lane, Norwich NR4 7UJ, UK

Tóm tắt

AbstractThe effectiveness of 200‐ and 400‐m isolation distances were evaluated for small‐scale trials of oilseed rape (Brassica napus L.). These distances have been used for previous transgenic release experiments and are commonly adopted for production of basic and certified breeders seed. A 400‐m2 donor plot contained plants with a dominant transgene conferring resistance to the herbicide glufosinate‐ammonium. This character was used as a selectable marker to measure the frequency of hybrid formation in 400‐m2 target plots of non‐transgenic rape. Seeds produced by the non‐transgenic plants were permitted to fall onto the plots and allowed to germinate. At the first true‐leaf stage, the plants were sprayed with glufosinate‐ammonium. Surviving plants were resprayed, and a subsample assayed for the presence of the transgene using a colorimetric assay and Southern‐blot analysis. The average frequency of hybridization over two replicates was 0.0156% at 200 m and 0.0038% at 400 m. These estimates are within the limits established for the production of basic seed (0.1%). Results indicated that bees were the most likely agent for long‐distance pollen dispersal.

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Plant Breeding

Tập 114 Số 4

317-321

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