Etudes Cytochimiques et Ultrastructurales de la Paroi de Candida albicans
Tóm tắt
La localisation ultrastructurale des polysaccharides paritétaux de Candida albicans a été réalisée par utilisation de Concanavaline A sur sections ultrafines de résine hydrosoluble (glycol méthacrylate). Les sites récepteurs de la lectine sont révélés en incubant successivement les coupes avec de la péroxydase, puis un mélange 3-3′ diaminobenzidine-H2O2. Cette méthode a permis de localiser les mannanes, qui sont les seuls polysaccharides pariétaux de C. albicans susceptibles de réagir avec la Concanavaline A. Deux couches continues réactives ont été ainsi mises en évidence à la périphérie de la paroi des blastospores. Les résultats sont discutés en fonction de ceux obtenus par une méthode différente de mise en évidence des polysaccharides (PATAg).
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