Ethnopharmacological evaluation of schistosomicidal and cercaricidal activities of some selected medicinal plants from Ghana

Tropical Medicine and Health - Tập 48 Số 1 - 2020
Desmond Omane Acheampong1, Ninette Owusu-Adzorah1, Francis Ackah Armah1, Enoch Aninagyei2, Ernest Amponsah Asiamah1, Ama Kyeraa Thomford1, William K. Anyan3
1Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Science, University of Cape Coast, Cape Coast, Ghana
2Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Volta Region, Ghana
3Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Ghana

Tóm tắt

Abstract Background The adulticidal and cercaricidal activities of five Ghanaian medicinal plants, namely, Phyllanthus amarus, Vernonia amygdalina, Azadirachta indica, Morinda lucida and Nauclea latifolia against S. mansoni were evaluated in this study. Six weeks old ICR mice (n = 25) were percutaneously infected with S. mansoni cercariae. Nine weeks later, infected mice (n = 5) were anaesthetised and perfused for adult S. mansoni. Cercariae were treated with different concentrations (1000, 500, 250, 125, 62.5, 31.25 μg/mL) of methanolic extracts of the experimenting plants in triplicates. Adult S. mansoni incopula were also treated with same concentrations of each extract or 20 μg/mL praziquantel. The cercariae and adult worms were observed at time intervals for 180 min and 120 h to assess mortality and viability respectively. Additionally, 9-week cercariae-infected mice (4 groups of 5 mice) were treated with either 500 mg/kg po A. indica or V. amygdalina, 400 mg/kg po praziquantel or distilled water for 14 days. The mice were euthanized after adult worms were recovered from them. The liver was processed and histologically examined for granuloma formations. Results All the plants exhibited varying cercaricidal and adulticidal activities against S. mansoni in a time and concentration-dependent manner. A. indica (3 h IC50 = 27.62 μg/mL) and V. amygdalina (3 h IC50 = 35.84 μg/mL) exerted the highest cercaricidal activity. Worm recovery after treatment with V. amygdalina, A. indica and praziquantel in vivo was 48.8%, 85.1 % and 59.9 % respectively (p < 0.05). A. indica and V. amydalina-treated mice recorded lesser mean liver and spleen weights compared to untreated groups (p < 0.05). Conclusion A. indica demonstrated the highest cercaricidal and alduticidal activities in vitro, whereas V. amygdalina exhibited the most potent aldulticidal activity in vivo. This study could provide baseline information which can be used to develop plant-based alternative commercial drugs against S. mansoni.

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Tài liệu tham khảo

Hotez PJ. NTDs V. 2.0:“blue marble health”—neglected tropical disease control and elimination in a shifting health policy landscape. PLOS Negl Trop Dis. 2013;7(11):e2570. https://doi.org/10.1371/journal.pntd.0002570.

King CH, Olbrych SK, Soon M, Singer ME, Carter J, Colley DG. Utility of repeated praziquantel dosing in the treatment of schistosomiasis in high-risk communities in Africa: a systematic review. PLOS Negl Trop Dis. 2011;5(9):e1321.

Thétiot-Laurent SAL, Boissier J, Robert A, Meunier B. Schistosomiasis chemotherapy. Angew Chem. 2013;52(31):7936–56.

Misra L, Wouatsa NV, Kumar S, Kumar RV, Tchoumbougnang F. Antibacterial, cytotoxic activities and chemical composition of fruits of two Cameroonian Zanthoxylum species. J Ethnopharmacol. 2013;148(1):74–80.

WHO. World malaria report 2014. Geneva: World Health Organization; 2015.

Stete K, Krauth SJ, Coulibaly JT, Knopp S, Hattendorf J, Müller I, Utzinger J. Dynamics of Schistosoma haematobium egg output and associated infection parameters following treatment with praziquantel in school-aged children. Parasit Vectors. 2012;5:298. https://doi.org/10.1186/1756-3305-5-298.

Farid Z. Schistosomes with terminal-spined eggs: pathological and clinical aspects. Biology. 1993:159–93.

Olds G, King C, Hewlett J, Olveda R, Wu G, Ouma J, Koech D. Double-blind placebo-controlled study of concurrent administration of albendazole and praziquantel in schoolchildren with schistosomiasis and geohelminths. J Infect. 1999;179(4):996–1003.

Anto F, Asoala V, Adjuik M, Anyorigiya T, Oduro A, Akazili J, et al. Water contact activities and prevalence of schistosomiasis infection among school-age children in communities along an irrigation scheme in rural northern Ghana. J Bacteriol Parasitol. 2013;4:117. https://doi.org/10.4172/2155-9597.1000177.

Bosompem KM, Bentum IA, Otchere J, Anyan WK, Brown CA, Osada Y, et al. Infant schistosomiasis in Ghana: a survey in irrigation community. Trop Med Int Health. 2004;9(8):917–22.

Wang T, Zhao M, Rotgans BA, Strong A, Liang D, Ni G, et al. Proteomic analysis of the Schistosoma mansoni Miracidium. PLoS ONE. 2016;11(1). https://doi.org/10.1371/journal.pone.0147247.

Rates SMK. Plants as source of drugs. Toxicon. 2001;39(5):603–13.

Dhama K, Tiwari R, Chakraborty S, Saminathan M, Kumar A, Karthik K, Rahal A. Evidence based antibacterial potentials of medicinal plants and herbs countering bacterial pathogens especially in the era of emerging drug resistance: an integrated update. Int J pharmacol. 2014;10(1):1–43.

Truiti MDT, Sarragiotto MH, Abreu FBA, Nakamura CV, Dias FBP. In vitro antibacterial activity of a 7-O-β-D-glucopyranosyl-nutanocoumarin from Chaptialia nutans (Asteraceae). Mem. Inst. Oswaldo Cruz. 2003;98:283–6.

Oliveira CN, Frezza TF, Garcia VL, Allergretti S. Schistosoma mansoni: in vivo evaluation of Phyllanthus amarus hexanic and ethanolic extracts. Experimental Parasitology. 2017;183.

Agyare C, Spiegler V, Sarkodie H, Asase A, Liebau E, Hensel A. An ethnopharmacological survey and in vitro confirmation of the ethnopharmacological use of medicinal plants as anthelmintic remedies in the Ashanti region, in the central part of Ghana. J Ethnopharmacol. 2014;158:255–63.

Groning E, Bakathir H, Salem A, Albert L, Fernández R. Efectividade y tolerancia de praziquantel. Rev Cub Med Trop. 1985;37:215–9.

Herwaldt BL, Tao L, Van Pelt W, Tsang VC, Bruce JI. Persistence of Schistosoma haematobium infection despite multiple courses of therapy with praziquantel. Clin Infect Dis. 1995;20:309–15.

Hatz CF, Vennervald BJ, Nkulila T, Vounatsou P, Kombe Y, Mayombana C, Mshinda H, Tanner M. Evolution of Schistosoma haematobium - related pathology over 24 months after treatment with praziquantel among school children in southeastern Tanzania. Am J Trop Med Hyg. 1998;59:775–81.

Ernould JC, Ba K, Sellin B. Increase of intestinal schistosomiasis after praziquantel treatment in Schistosoma haematobium and Schistosoma mansoni mixed focus. Acta Trop. 1999;73:143–52.

Liang YS, Coles GC, Doenhoff MJ. Short communication: Detection of praziquantel resistance in schistosomiasis. Trop Med Int Health. 2000;5:72.

Abara SM. Treatment of schistosomiasis: from praziquantel to development of new drug targets. PUJ. 2013;6(2):127–48.

Musili R, Muregi F, Mwatha J, Muriu D, M’Rewa L, Kamau T, Menaine A, Chege S, Thiong’o J, Ng’ang’a Z, Kimani G. Antischistosomal activity of Azadirachta Indica and Ekebergia capensis in mice infected with Schistosoma mansoni. Eur J Med Plants. 2015;6(2):92–102.

Quelemes PV, Perfeito ML, Guimarães MA, dos Santos RC, Lima DF, Nascimento C, Silva MP, Soares MJ, Ropke CD, Eaton P, et al. Effect of neem (Azadirachta indica A. Juss) leaf extract on resistant Staphylococcus aureus biofilm formation and Schistosoma mansoni worms. J Ethnopharmacol. 2015;175:287–94.

Adediran OA, Uwalaka EC. Effectiveness evaluation of levamisole, albendazole, ivermectin, and Vernonia amygdalina in West African dwarf goats. J Parasitol Res. 2015. https://doi.org/10.1155/2015/706824.

Ogboli A, Nock I, Obdurahman E, Ibrahim N. Medicinal application of Vernonia amygdalina del leaf extracts in the treatment of schistosomiasis in mice. Nigerian J Nat Products Med. 2000;4(1):73–5.

Yeap SK, Ho WY, Beh BK, San Liang W, Ky H, Yousr AHN, Alitheen NB. Vernonia amygdalina, an ethnoveterinary and ethnomedical used green vegetable with multiple bio-activities. J Med Plant Res. 2010;4(25):2787–812.

Burke JM, Jones MK, Gobert GN, Li YS, Ellis MK, McManus DP. Immunopathogenesis of human schistosomiasis. Parasite Immunol. 2009;31:163–76.

Ross AG, Olds GR, Cripps AW, Farrar JJ, McManus DP. Enteropathogens and chronic illness in returning travellers. N Engl J. Med. 2013;368:1817–25.

Deng YX, Cao M, Shi DX, Yin ZQ, Jia RY, Xu J, Wang C, Lv C, Liang XX, He CL, Yang ZR, Zhao J. Toxicological evaluation of neem (Azadirachta indica) oil: acute and subacute toxicity. Environ Toxicol Pharmacol. 2013;35(2):240–6.

Akowuah GA, May LLY, Chin JH. Toxicological evaluation of Vernonia amygdalina methanol leave extract in rats. Orient Pharm Exp Med. 2015;15:365–9. https://doi.org/10.1007/s13596-015-0194-6.

Eshetu M. G, Berhanu E. Laboratory assessment of the molluscicidal and cercariacidal activities of Balanites aegyptiaca. Asian Pac J Trop Biomed. 2013;3(8):657–62.

Koko WS, Abdalla HS, Galal M, Khalid HS. Evaluation of oral therapy on mansomal schistosomiasis using single dose of Balanites aegyptiaca fruits and praziquantel. Fitoterapia. 2005;76:30–4.

Anowi CF, Cardinal NC, Mbah C, Onyekaba T. Antimicrobial properties of the methanolic extract of the leaves of Nauclea latifolia. IJDRT. 2017;2(1):10.

Sturrock B, Sturrock R. Laboratory studies of the host-parasite relationship of Schistosoma mansoni and Biomphalaria glabrata from St Lucia, West Indies. Ann Trop Med PH. 1970;64(3):357–63.

Saleh M, Glombitza K. Volatile oil of Marrubium vulgare and its anti-schistosomal activity. Planta Medica. 1989;55(01):105.

Gyasi SF, Awuah E, Asiedu LJA, Koffuor GA, Debrah AY, Awua-Boateng NY, Osei O-A. Susceptibility of arsenic-exposed ICR mice to buruli ulcer development. Pharmacologia. 2013;4:254–64.

Mahmoud M, El-Abhar H, Saleh S. The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. J Ethnopharmacol. 2002;79(1):1–11.

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Tropical Medicine and Health

Tập 48 Số 1

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