Estrogen Induces Insulin-Like Growth Factor-1 mRNA Expression in the Immortalized Hippocampal Cell: Determination by Quantitative Real-Time Polymerase Chain Reaction

Neurochemical Research - Tập 28 Số 9 - Trang 1379-1383 - 2003
Shingo, Akiko S.1, Kito, Shozo1
1Hyogo University School of Health Sciences, Hyogo, Japan

Tóm tắt

The basic mechanisms of nerve protection by estrogen remain to be clarified. This study was undertaken to confirm estrogen-induced insulin-like growth factor 1 (IGF-1) mRNA expression in the immortalized rat hippocampal cell H19-7 using a real-time quantitative polymerase chain reaction (PCR) assay, which has considerably increased accuracy and rapidity over other current methods. Upon stimulation by estradiol, the copy number of ERα mRNA showed a 1.4-fold increase, and that of IGF-1 mRNA showed a 38.5-fold increase when compared with the control value. ICI182,780 inhibited the estradiol-induced upregulation of ERα RNA completely, while it inhibited estradiol-stimulated IGF-1 mRNA expression partially. The increase of the copy number of IGF-1 mRNA was accomanied by enhancement of IGF-1 protein as observed by Western blot analysis.

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Neurochemical Research

Tập 28 Số 9

1379-1383

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