Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent

Nature Protocols - Tập 2 Số 4 - Trang 875-877 - 2007
Elizabeth A. Ainsworth1, Kelly M. Gillespie2
1USDA ARS Photosynthesis Research Unit, Urbana, 61801, Illinois, USA
2Program in Physiological and Molecular Plant Biology and Institute for Genomic Biology, University of Illinois Urbana-Champaign, 147 ERML, 1201 W. Gregory Drive, Urbana, 61801, Illinois, USA

Tóm tắt

Từ khóa


Tài liệu tham khảo

Croteau, R., Kutchan, T.M. & Lewis, N.G. Natural products (secondary metabolites). In Biochemistry & Molecular Biology of Plants (eds. Buchanan, B., Gruissem, W. & Jones, R.L.) 1250–1318 (American Society of Plant Physiologists, Beltsville, MD, USA, 2000).

Grace, S.C. & Logan, B.A. Energy dissipation and radical scavenging by the plant phenylpropanoid pathway. Phil. Trans. R. Soc. Lond. B 355, 1499–1510 (2000).

Grace, S.C. Phenolics as antioxidants. In Antioxidants and Reactive Oxygen Species in Plants (ed. Smirnoff, N.) 141–168 (Blackwell Publishing, Oxford, UK, 2005).

Bors, W., Heller, W., Michel, C. & Saran, M. Flavonoids as antioxidants: determination of radical-scavenging efficiencies. Methods Enzymol. 186, 343–355 (1990).

Bors, W., Michel, C. & Saran, M. Flavonoid antioxidants: rate constants for reactions with oxygen radicals. Methods Enzymol. 234, 420–429 (1994).

Dixon, R.A. & Paiva, N.L. Stress-induced phenylpropanoid metabolism. Plant Cell 7, 1085–1097 (1995).

Pasqualini, S. et al. Ozone-induced cell death in tobacco cultivar Bel W3 plants. The role of programmed cell death in lesion formation. Plant Physiol. 133, 1122–1134 (2003).

Robards, K. & Antolovich, M. Analytical chemistry of fruit bioflavonoids a review. Analyst 122, 11R–34R (1997).

Magalhães, L.M., Segundo, M.A., Reis, S., Lima, J.L. & Rangel, A.O. Automatic method for the determination of Folin–Ciocalteu reducing capacity in food products. J. Agric. Food Chem. 54, 5241–5246 (2006).

Folin, O. & Ciocalteu, V. On tyrosine and tryptophane determinations in proteins. J. Biol. Chem. 73, 627–650 (1927).

Singleton, V.L. & Rossi, J.A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16, 144–158 (1965).

Prior, R.L., Wu, X. & Schaich, K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agric. Food Chem. 53, 4290–4302 (2005).

Singleton, V.L., Orthofer, R. & Lamuela-Raventós,, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299, 152–178 (1999).

Huang, D., Ou, B. & Prior, R.L. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53, 1841–1856 (2005).

Booker, F.L. & Miller, J.E. Phenylpropanoid metabolism and phenolic composition of soybean [Glycine max (L.) Merr.] leaves following exposure to ozone. J. Exp. Bot. 49, 1191–1202 (1998).

Gillespie, K.M., Chae, J.M. & Ainsworth, E.A. Rapid measurement of total antioxidant capacity in plants. Nat. Protoc. 2, 867–870 (2007).

Gillespie, K.M. & Ainsworth, E.A. Measurement of reduced, oxidized and total ascorbate content in plants. Nat. Protoc. 2, 871–874 (2007).