Enzymatic hydrolysis of soy and chickpea protein with Alcalase and Flavourzyme and formation of hydrogen bond mediated insoluble aggregates

Ahmed, 2006, Thermorheological characteristics of soybean protein isolate, J. Food Sci., 71, E158, 10.1111/j.1365-2621.2006.tb15629.x Alonso-Miravalles, 2019, Membrane filtration and isoelectric precipitation technological approaches for the preparation of novel, functional and sustainable protein isolate from lentils, Eur. Food Res. Technol., 245, 1855, 10.1007/s00217-019-03296-y Boye, 2010, Comparison of the functional properties of pea, chickpea and lentil protein concentrates processed using ultrafiltration and isoelectric precipitation techniques, Food Res. Int., 43, 537, 10.1016/j.foodres.2009.07.021 Bradford, 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 Brishti, 2020, Effects of drying techniques on the physicochemical, functional, thermal, structural and rheological properties of mung bean (Vigna radiata) protein isolate powder, Food Res. Int., 138, 10.1016/j.foodres.2020.109783 Carbonaro, 2010, Secondary structure of food proteins by Fourier transform spectroscopy in the mid-infrared region, Amino Acids, 38, 679, 10.1007/s00726-009-0274-3 Chang, 2012, Isolation and characterization of chickpea (Cicer arietinum L.) seed protein fractions, Food Bioprocess Technol., 5, 618, 10.1007/s11947-009-0303-y Chen, 2022, Limited enzymatic hydrolysis induced pea protein gelation at low protein concentration with less heat requirement, Food Hydrocolloids, 10.1016/j.foodhyd.2022.107547 Chen, 2021, Polyphenols weaken pea protein gel by formation of large aggregates with diminished noncovalent interactions, Biomacromolecules, 22, 1001, 10.1021/acs.biomac.0c01753 Creusot, 2007, Hydrolysis of whey protein isolate with Bacillus licheniformis protease: fractionation and identification of aggregating peptides, J. Agric. Food Chem., 55, 9241, 10.1021/jf071584s del Mar Yust, 2010, Improvement of functional properties of chickpea proteins by hydrolysis with immobilized Alcalase, Food Chem., 122, 1212, 10.1016/j.foodchem.2010.03.121 del Mar Yust, 2013, Hydrolysis of chickpea proteins with Flavourzyme immobilized on glyoxyl-agarose gels improves functional properties, Food Sci. Technol. Int., 19, 217, 10.1177/1082013212442197 Dent, 2022, Pulse protein processing: the effect of processing choices and enzymatic hydrolysis on ingredient functionality, Crit. Rev. Food Sci. Nutr., 1 do Evangelho, 2017, Black bean (Phaseolus vulgaris L.) protein hydrolysates: physicochemical and functional properties, Food Chem., 214, 460, 10.1016/j.foodchem.2016.07.046 Eildal, 2013, Probing the role of backbone hydrogen bonds in protein–peptide interactions by amide-to-ester mutations, J. Am. Chem. Soc., 135, 12998, 10.1021/ja402875h Fischer, 2002, Aggregation of peptides during hydrolysis as a cause of reduced enzymatic extractability of soybean meal proteins, J. Agric. Food Chem., 50, 4512, 10.1021/jf0114785 Ghribi, 2015, Effects of enzymatic hydrolysis on conformational and functional properties of chickpea protein isolate, Food Chem., 187, 322, 10.1016/j.foodchem.2015.04.109 Graça, 2015, Meat, beyond the plate. Data-driven hypotheses for understanding consumer willingness to adopt a more plant-based diet, Appetite, 90, 80, 10.1016/j.appet.2015.02.037 Horax, 2017, Solubility, functional properties, ACE‐I inhibitory and DPPH scavenging activities of Alcalase hydrolysed soy protein hydrolysates, Int. J. Food Sci. Technol., 52, 196, 10.1111/ijfs.13267 Kong, 2007, Fourier transform infrared spectroscopic analysis of protein secondary structures, Acta Biochim. Biophys. Sin., 39, 549, 10.1111/j.1745-7270.2007.00320.x Kaur, 2007, Characterization of protein isolates from different Indian chickpea (Cicer arietinum L.) cultivars, Food Chem., 102, 366, 10.1016/j.foodchem.2006.05.029 Kuipers, 2008, Identification of strong aggregating regions in soy glycinin upon enzymatic hydrolysis, J. Agric. Food Chem., 56, 3818, 10.1021/jf703781j Ladjal-Ettoumi, 2016, Pea, chickpea and lentil protein isolates: physicochemical characterization and emulsifying properties, Food Biophys., 11, 43, 10.1007/s11483-015-9411-6 Loveday, 2020, Plant protein ingredients with food functionality potential, Nutr. Bull., 45, 321, 10.1111/nbu.12450 Liu, 2008, Protein–protein interactions during high-moisture extrusion for fibrous meat analogues and comparison of protein solubility methods using different solvent systems, J. Agric. Food Chem., 56, 2681, 10.1021/jf073343q Meinlschmidt, 2015, Enzymatic treatment of soy protein isolates: effects on the potential allergenicity, technofunctionality, and sensory properties, Food Sci. Nutr., 4, 11, 10.1002/fsn3.253 Merz, 2015, Flavourzyme, an enzyme preparation with industrial relevance: automated nine-step purification and partial characterization of eight enzymes, J. Agric. Food Chem., 63, 5682, 10.1021/acs.jafc.5b01665 Nasrabadi, 2021, Modification approaches of plant-based proteins to improve their techno-functionality and use in food products, Food Hydrocolloids, 118 Nguyen, 2016, Physicochemical properties and ACE-I inhibitory activity of protein hydrolysates from a non-genetically modified soy cultivar, J. Am. Oil Chem. Soc., 93, 595, 10.1007/s11746-016-2801-1 Nielsen, 2001, Improved method for determining food protein degree of hydrolysis, J. Food Sci., 66, 642, 10.1111/j.1365-2621.2001.tb04614.x O′ Flynn, 2021, Rheological and solubility properties of soy protein isolate, Molecules, 26, 3015, 10.3390/molecules26103015 Orlando, 2021 Otegui, 1997, Properties of spray‐dried and freeze‐dried faba bean protein concentrates, Int. J. Food Sci. Technol., 32, 439, 10.1111/j.1365-2621.1997.tb02118.x Otte, 1996, Protease‐induced aggregation and gelation of whey proteins, J. Food Sci., 61, 911, 10.1111/j.1365-2621.1996.tb10900.x Papalamprou, 2009, Influence of preparation methods on physicochemical and gelation properties of chickpea protein isolates, Food Hydrocolloids, 23, 337, 10.1016/j.foodhyd.2008.03.006 Peña‐Ramos, 2002, Antioxidant activity of soy protein hydrolysates in a liposomal system, J. Food Sci., 67, 2952, 10.1111/j.1365-2621.2002.tb08844.x Renkema, 2002, Influence of pH and ionic strength on heat-induced formation and rheological properties of soy protein gels in relation to denaturation and their protein compositions, J. Agric. Food Chem., 50, 6064, 10.1021/jf020061b Rivera, 2022, A comprehensive review on pulse protein fractionation and extraction: processes, functionality, and food applications, Crit. Rev. Food Sci. Nutr., 1, 10.1080/10408398.2022.2139223 Rosi, 2017, Environmental impact of omnivorous, ovo-lacto-vegetarian, and vegan diet, Sci. Rep., 7, 1, 10.1038/s41598-017-06466-8 Sha, 2020, Plant protein-based alternatives of reconstructed meat: science, technology, and challenges, Trends Food Sci. Technol., 102, 51, 10.1016/j.tifs.2020.05.022 Villanueva, 1999, Production of an extensive sunflower protein hydrolysate by sequential hydrolysis with endo-and exo-proteases, Grasas Aceites (Seville), 50 Wang, 2014, Relationship between secondary structure and surface hydrophobicity of soybean protein isolate subjected to heat treatment, J. Chem., 10.1155/2014/475389 Waterborg, 1994, 1 Xie, 2019, Physico-chemical properties, antioxidant activities and angiotensin-I converting enzyme inhibitory of protein hydrolysates from Mung bean (Vigna radiate), Food Chem., 270, 243, 10.1016/j.foodchem.2018.07.103 Xu, 2020, Effect of enzymatic hydrolysis using endo-and exo-proteases on secondary structure, functional, and antioxidant properties of chickpea protein hydrolysates, J. Food Meas. Char., 14, 343, 10.1007/s11694-019-00296-0 Yang, 2016, Secondary structure and subunit composition of soy protein in vitro digested by pepsin and its relation with digestibility, BioMed Res. Int. Yokoyama, 2017, Association between plant-based diets and plasma lipids: a systematic review and meta-analysis, Nutr. Rev., 75, 683, 10.1093/nutrit/nux030 Zhang, 2017, Soy peptide aggregates formed during hydrolysis reduced protein extraction without decreasing their nutritional value, Food Funct., 8, 4384, 10.1039/C7FO00812K Zhao, 2013, Changes in structural characteristics of antioxidative soy protein hydrolysates resulting from scavenging of hydroxyl radicals, J. Food Sci., 78, C152, 10.1111/1750-3841.12030 Zheng, 2015, Mechanism of the discrepancy in the enzymatic hydrolysis efficiency between defatted peanut flour and peanut protein isolate by Flavorzyme, Food Chem., 168, 100, 10.1016/j.foodchem.2014.07.037