Enhancement of flavone levels through overexpression of chalcone isomerase in hairy root cultures of Scutellaria baicalensis

Chi YS, Cheon BS, Kim HP (2001) Effect of wogonin, a plant flavone from Scutellaria radix, on the suppression of cyclooxygenase-2 and the induction of inducible nitric oxide synthase in lipopolysaccharide-treated RAW 264.7 cells. Biochem Pharmacol 61:1195–1203 Chung H, Y-m J, Shin D-H, Lee J-Y, Oh M-Y, Kim H-J, Jang KS, Jeon SJ, Son KH, Kong G (2008) Anticancer effects of wogonin in both estrogen receptor-positive and -negative human breast cancer cell lines in vitro and in nude mice xenografts. Int J Cancer 122:816–822 Creelman RA, Tierney ML, Mullet JE (1992) Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc Natl Acad Sci USA 89:4938–4941 Giri A, Narasu ML (2000) Transgenic hairy roots: recent trends and applications. Biotechnol Adv 18:1–22 Guillon S, Tremouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006) Hairy root research: recent scenario and exciting prospects. Curr Opin Plant Biol 9:341 Han J, Ye M, Xu M, Sun JH, Wang BR, Guo D (2007) Characterization of flavonoids in the traditional Chinese herbal medicine-Huangqin by liquid chromatography coupled with electrospray ionization mass spectrometry. J Chromatogr B 848:355–362 Huang Y, Tsang SY, Yao X, Chen ZY (2005) Biological properties of baicalein in cardiovascular system. Curr Drug Targets Cardiovasc Haematol Disord 5:177–184 Junghans H, Dalkin K, Dixon RA (1993) Stress responses In alfalfa (Medicago sativa L).15. Characterization and expression patterns of members of a subset of the chalcone synthase multigene family. Plant Mol Biol 22:239–253 Kovács G, Kuzovkina IN, Szoke É, Kursinszki L (2004) HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi. Chromatographia 60:S81–S85 Kuzovkina IN, Guseva AV, Alterman IE, Karnachuk RA (2001) Flavonoid production in transformed Scutellaria baicalensis roots and ways of its regulation. Russ J Plant Physiol 48:448–452 Kuzovkina IN, Guseva AV, Kovács D, Szöke É, Vdovitchenko MY (2005) Flavones in genetically transformed Scutellaria baicalensis roots and induction of their synthesis by elicitation with methyl jasmonate. Russ J Plant Physiol 52:77–82 Lee H, Kim YO, Kim H, Kim SY, Noh HS, Kang SS, Cho GJ, Choi WS, Suk K (2003) Flavonoid wogonin from medicinal herb is neuroprotective by inhibiting inflammatory activation of microglia. FASEB J 17:1943–1944. doi:10.1096/fj.03-0057fje Lee D-H, Kim C, Zhang L, Lee YJ (2008) Role of p53, PUMA, and Bax in wogonin-induced apoptosis in human cancer cells. Biochem Pharmacol 75:2020–2033 Li H, Murch S, Saxena P (2000) Thidiazuron-induced de novo shoot organogenesis on seedlings, etiolated hypocotyls and stem segments of Huang-qin. Plant Cell Tissue Organ Cult 62:169–173 Li F-X, Jin Z-P, Qu W, Zhao D-X, Ma F (2006a) Cloning of cDNA encoding the Saussurea medusa chalcone isomerase and its expression in transgenic tobacco. Plant Physiol Biochem 44:455–461 Li F-X, Jin Z-P, Zhao D-X, Cheng L-Q, Fu C-X, Ma F (2006b) Overexpression of the Saussurea medusa chalcone isomerase gene in S. involucrata hairy root cultures enhances their biosynthesis of apigenin. Phytochemistry 67:553–560 Liu S, Zhang J, Li D, Liu W, Luo X, Zhang R, Li L, Zhao J (2007) Anticancer activity and quantitative analysis of flavone of Cirsium japonicum DC. Nat Prod Res formerly Nat Prod Lett 21:915–922 Li-Weber M (2009) New therapeutic aspects of flavones: the anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat Rev 35:57–68 Lu N, Gao Y, Ling Y, Chen Y, Yang Y, Gu H-Y, Qi Q, Liu W, Wang X-T, You Q-D, Guo Q-L (2008) Wogonin suppresses tumor growth in vivo and VEGF-induced angiogenesis through inhibiting tyrosine phosphorylation of VEGFR2. Life Sci 82:956–963 Murch SJ, Rupasinghe HPV, Goodenowe D, Saxena PK (2004) A metabolomic analysis of medicinal diversity in Huang-qin (Scutellaria baicalensis Georgi) genotypes: discovery of novel compounds. Plant Cell Rep 23:419–425 Nishikawa K, Furukawa H, Fujioka T, Fujii H, Mihashi K, Shimomura K, Ishimaru K (1999) Flavone production in transformed root cultures of Scutellaria baicalensis Georgi. Phytochemistry 52:885–890 Parajuli P, Joshee N, Rimando AM, Mittal S, Yadav AK (2009) In vitro antitumor mechanisms of various Scutellaria extracts and constituent flavonoids. Planta Med 75:41–48 Richard S, Lapointe G, Rutledge RG, Seguin A (2000) Induction of chalcone synthase expression in white spruce by wounding and jasmonate. Plant Cell Physiol 41:982–987. doi:10.1093/pcp/pcd017 Shieh DE, Liu LT, Lin CC (2000) Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. Anticancer Res 20:2861–2865 Shimada N, Aoki T, Sato S, Nakamura Y, Tabata S, S-i A (2003) A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxy(iso)flavonoids in Lotus japonicus. Plant Physiol 131:941–951. doi:10.1104/pp.004820 Wang W, Guo Q-L, You Q-D, Zhang K, Yang Y, Yu J, Liu W, Zhao L, Gu H-Y, Hu Y, Tan Z, Wang X-T (2006) The anticancer activities of wogonin in murine sarcoma S180 both in vitro and in vivo. Biol Pharm Bull 29:1132–1137 Wasson AP, Pellerone FI, Mathesius U (2006) Silencing the flavonoid pathway in Medicago truncatula inhibits root nodule formation and prevents auxin transport regulation by rhizobia. Plant Cell 18:1617–1629. doi:10.1105/tpc.105.038232 Xu H, Park NI, Li X, Kim YK, Lee SY, Park SU (2010) Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis. Bioresour Technol 101:9715–9722 Zhang H-C, Liu J-M, Lu H-Y, Gao S-L (2009) Enhanced flavonoid production in hairy root cultures of Glycyrrhiza uralensis Fisch by combining the over-expression of chalcone isomerase gene with the elicitation treatment. Plant Cell Rep 28:1205–1213