Enhancement of Kraft lignin molecular relaxation based on laccases from Pycnoporus sanguineus produced in instrumented bioreactors
Tóm tắt
This study has been focused on obtaining fungal laccases and structural modification of lignin by enzymatic action in laccase-ABTS system, mainly reducing molecular stiffness. The best specific aeration rate in bubble column reactor is 0.67 vvm, with 3260 U/L laccase activity, and enzymatic activity increased by 60% in stirred tank reactor. Enzymatic cocktail has been concentrated in a hollow fiber system and partially characterized. Laccase-ABTS system promoted significant thermal and structural changes in Kraft lignin in all essayed reaction times (2, 24, 48, and 72 h). The best reaction time of the enzymatic treatment is LT-lac48, which promoted the highest relaxation coefficient (18.0%). The enzymatic cocktail produced herein is efficient for structural modification of Kraft lignin. The positive evidence of lignin’s relaxation/flexibility and its linearization and homogenization suggests that treatment performed may adjust studied lignin to produce high-performance lignin suitable for many applications.
Tài liệu tham khảo
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