Enhancement of Alcohol Metabolism by Ginseng Berry Extract and its Mixed Herbal Beverages: In vitro and in vivo Experiments
Tóm tắt
To investigate the enhancement of alcohol metabolism by two ginseng berry (GB) extracts and their two types of mixed herbal beverages through in vitro and in vivo experiments. Two GB extract solutions and their two herbal beverages were evaluated as enhancers of alcohol metabolism in normal human embryonic liver cells (CL-48 cell line) through assays of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activity. Cytotoxicity was also assessed in the same cell line using an MTT assay. Effects on alcohol metabolism were also observed in vivo through measurement of serum alcohol, acetaldehyde, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels in alcohol treated rats. Blood samples were serially collected at 1, 2, 3, 4, 6 and 8 hrs after a single ethanol (EtOH) treatment. A single treatment with the test samples was administered orally 30 minutes after EtOH treatment. GB extract solutions effectively elevated the ADH and ALDH activity observed in vitro, while no treatment- related cytotoxic effects were found with test samples t concentrations up to 100 mg/mL. Significantly lower (p<0.01 or p<0.05) serum alcohol and acetaldehyde content was observed in samples from treated rats than in those from control rats (EtOH only) 1 or 2 hrs after EtOH treatment. In addition, noticeable decreases were observed in serum AST and ALT levels in treated samples 8 hrs after EtOH administration. HM40, an herbal mixture containing GB extract (40 mg/75 mL of ginsenoside Re), showed betters enhancement of alcohol metabolism through ADH/ALDH activation, as well as related hepatoprotective effects. GB extracts effectively enhanced alcohol metabolism without cytotoxicity while also providing possible hepatoprotective effects that could serve as a functional ingredient in anti-hangover alternative therapies. These extracts are expected to be more effective when made into herbal mixture beverages.
Tài liệu tham khảo
Jung, T. W. et al. Rosiglitazone relieves acute ethanol- induced hangover in Sprague-Dawley rats. Alcohol 41, 231–235 (2006).
Verster, J. C. The alcohol hangover — a puzzling phenomenon. Alcohol 43, 124–126 (2008).
Prat, G., Adan, A. & Sanchez-Turet, M. Alcohol hangover: a critical review of explanatory factors. Hum. Psychopharmacol. 24, 259–257 (2009).
An, S. W. et al. Comparison of hepatic detoxification activity and reducing serum alcohol concentration of Hovenia dulsis Thunb and Alnus japonica Steud. Korean J. Med. Crop Sci. 7, 263–268 (1999).
Sung, H. M. et al. Effect of a soy-sprout beverage prepared with high-concentrated oxygen water on alcohol metabolism in rats. Korean J. Food Sci. Technol. 46, 616–621 (2014).
Lee, D. I. et al. Ginsenoside-free molecules from steamdried ginseng berry promote ethanol metabolism: an alternative choice for an alcohol hangover. J. Food Sci. 79, C1323–C1330 (2014).
McGregor, N. R. Pueraria lobata (kudzu root) hangover remedies and acetaldehyde-associated neoplasm risk. Alcohol 41, 469–478 (2007).
Wojciech, L., Ewa, Z. & Elzbieta, S. Influence of green tea on erythrocytes antioxidant status of different age rats intoxicated with ethanol. Phytother. Res. 4, 424–428 (2010).
Sanada, S., Kondo, N., Shoji, J., Tanaka, O. & Shibata, S. Studies on the saponins of ginseng. I. Structures of ginsenoside-Ro,-Rb1,-Rb2,-Rc and -Rd. Chem. Pharm. Bull. 22, 421–428 (1974).
Park, J. D. Recent studies on the chemical constituents of Korean ginseng (Panax ginseng C. A. Meyer). Korean J. Ginseng Sci. 20, 389–415 (1996).
Tanaka, O., Nagai, M. & Shibata, S. Chemical studies on the oriental plant drugs. XVI. The stereochemistry of protopanaxadiol, a genuine sapogenin of ginseng. Chem. Pharm. Bull. 14, 1150–1156 (1966).
Mochizuki, M. et al. Inhibitory effect of tumor metastasis in mice by saponins, ginsenoside-Rb2, 20(R)- and 20(S)-ginsenoside-Rg3, of red ginseng. Biol. Pharm. Bull. 18, 1197–1202 (1995).
Yokozawa, T., Kobayashi, T., Oura, H. & Kawashima, Y. Studies on the mechanism of the hypoglycemic activity of ginsenoside-Rb2 in streptozotocin-diabetic rats. Chem. Pharm. Bull. 33, 869–872 (1985).
Takagi, K., Saito, H. & Nabata, H. Pharmacological studies of Panax ginseng root: estimation of pharmacological actions of Panax ginseng root. Jpn. J. Pharmacol. 22, 245–249 (1972).
Jung, I. S. & Cho, Y. D. Effect of ginseng saponin fraction on absorption of cholesterol and serum lipid components. Korean J. Ginseng Sci. 9, 232–239 (1985).
Yoon, S. H. & Joo, C. N. Study on the preventive effect of ginsenosides against hypercholesterolemia and its mechanism. Korean J. Ginseng Sci. 17, 1–12 (1993).
Ki, S. H. et al. Red ginseng extract protects against carbon tetrachloride-induced liver fibrosis. J. Ginseng Res. 37, 45–53 (2013).
Park, S. J. et al. Protective effects of Korean red ginseng extract on cadmium-induced hepatic toxicity in rats. J. Ginseng Res. 37, 37–44 (2013).
Lee, M. H. et al. Red ginseng relieves the effects of alcohol consumption and hangover symptoms in healthy men: a randomized crossover study. Food Funct. 5, 528–534 (2014).
Saito, H., Yoshida, Y. & Takagi, K. Effect of Panax ginseng root on exhaustive exercise in mice. Jpn. J. Pharmacol. 24, 119–127 (1974).
Wang, B. X., Cui, J. C., Liu, A. J. & Wu, S. K. Studies on the anti-fatigue effect of the saponins of stems and leaves of Panax ginseng (SSLG). J. Tradit. Chin. Med. 3, 89–94 (1983).
Park, M. S. et al. Korean red ginseng protects oxidative injury caused by lead poisoning. J. Ginseng Res. 34, 132–137 (2010).
Matsuda, H., Samukawa, K. & Kubo, M. Anti-inflammatory activity of ginsenoside Ro. Planta Med. 56, 19–23 (1990).
Yokozawa, T. & Oura, H. Facilitation of protein biosynthesis by ginsenoside-Rb2 administration in diabetic rats. J. Nat. Prod. 53, 1514–1518 (1990).
Jie, Y. H., Cammisuli, S. & Baggiolini, M. Immunomodulatory effects of Panax ginseng C.A. Meyer in the mouse. Agents Actions 15, 386–391 (1984).
Bae, H. M. et al. Inhibitory effects of ginsenoside Re isolated from ginseng berry on histamine and cytokine release in human mast cells and human alveolar epithe lial cells. J. Ginseng Res. 36, 369–374 (2012).
Wang, C. Z. et al. Steamed American ginseng berry: ginsenoside analyses and anticancer activities. J. Agric. Food Chem. 54, 9936–9942 (2006).
Dey, L., Zhang, L. & Yuan, C. S. Anti-diabetic and antiobese effects of ginseng berry extract: comparison between intraperitoneal and oral administrations. Am. J. Chin. Med. 30, 645–647 (2002).
Xie, J. T. et al. American ginseng berry juice intake reduces blood glucose and body weight in ob/ob mice. J. Food Sci. 72, S590–S594 (2007).
Wang, W. et al. In vitro anti-cancer activity and structure- activity relationships of natural products isolated from fruits of Panax ginseng. Cancer Chemother. Pharmacol. 59, 589–601 (2007).
Mehendale, S. R. et al. Chronic pretreatment with American ginseng berry and its polyphenolic constituents attenuate oxidant stress in cardiomyocytes. Eur. J. Pharmacol. 553, 209–214 (2006).
Xie, J. T. et al. Antioxidant effects of ginsenoside Re in cardiomyocytes. Eur. J. Pharmacol. 532, 201–207 (2006).
Zhang, S. C. & Jiang, X. L. The anti-stress effect of saponins extracted from Panax ginseng fruit and the hypophyseal adrenal system. Yao Xue Xue Bao. 6, 860–863 (1981).
Lee, S. et al. Protective effect of ginsenoside Re on acute gastric mucosal lesion induced by compound 48–80. J. Ginseng Res. 38, 89–96 (2014).
Seo, J. Y., Kim, S. S. & Kim, J. S. Enhancement of alcohol metabolism by sprouted peanut extract in SD rats. Prev. Nutr. Food Sci. 19, 1–4 (2014).
Bosron, W. F. & Li, T. K. Genetic polymorphism of human liver alcohol and aldehyde dehydrogenases, and their relationship to alcohol metabolism and alcoholism. Hepatology 6, 502–510 (1986).
Smit, H. F. et al. Ayurvedic herbal drugs with possible cytostatic activity. J. Ethnopharmacol. 47, 75–84 (1995).
Kratzke, R. A. & Kramer, B. S. Evaluation of in vitro chemosensitivity using human lung cancer cell lines. J. Cell Biochem. Suppl. 24, 160–164 (1996).
Cho, S. Y. et al. Effects of chitooligosaccharide lactate salt on activity of acetaldehyde dehydrogenase. J. Med. Food 13, 1061–1068 (2010).
Lieber, C. S. Liver adaptation and injury in alcoholism. N. Engl. J. Med. 288, 356–362 (1973).
Helander, A. & Tottmar, O. Effect of acute ethanol administration on human blood aldehyde dehydrogenase activity. Alcohol Clin. Exp. Res. 12, 643–646 (1988).
Lieber, C. S. Alcohol and the liver: metabolism of ethanol, metabolic effects and pathogenesis of injury. Acta Med. Scand. Suppl. 703, 11–55 (1985).
Sodikoff, C. H. Laboratory profiles of small animal diseases. A guide to laboratory diagnosis. 2nd ed., Mosby: St. Louise, 1–36 (1995).
Pramyothin, P., Ngamtin, C., Poungshompoo, S. & Chaichantipyuth, C. Hepatoprotective activity of Phyllanthus amarus Schum. et. Thonn. extract in ethanol treated rats: in vitro and in vivo studies. J. Ethnopharmacol. 114, 169–173 (2007).
Bostian, K. A. & Betts, G. F. Rapid purification and properties of potassium-activated aldehyde dehydrogenase from Saccharomyces cerevisiae. Biochem. J. 173, 773–786.(1978).
Easterbrook, J., Fackett, D. & Li, A. P. A comparison of aroclor 1254-induced and uninduced rat liver microsomes to human liver microsomes in phenytoin O-deethylation, coumarin 7-hydroxylation, tolbutamide 4-hydroxylation, S-mephenytoin 4′-hydroxylation, chloroxazone 6 hydroxylation and testosterone 6beta-hydroxylation. Chem. Biol. Interact. 134, 243–249 (2001).
McCloskey, L. P. & Mahaney, P. An enzymatic assay for acetaldehyde in grape juice and wine. Am. J. Enol. Vitic. 32, 159–162 (1981).
Levene, A. Pathological factors influencing excision of tumours in the head and neck. Part I. Clin. Otolaryngol. Allied Sci. 6, 145–151 (1981).
Ludbrook, J. Update: microcomputer statistics packages. A personal view. Clin. Exp. Pharmacol. Physiol. 24, 294–296 (1997).
Kim, K. H. et al. Anti-skin-aging benefits of exopolymers from Aureobasidium pullulans SM-2001. J. Cosmet. Sci. 65, 285–298 (2014).