Enhanced production of cellulase, hemicellulase, and β‐glucosidase by Trichoderma reesei (Rut C‐30)

Biotechnology and Bioengineering - Tập 23 Số 8 - Trang 1837-1849 - 1981
S. Kishen Tangnu1, Harvey W. Blanch1, Charles R. Wilke1
1Lawrence Berkeley Laboratory and Department of Chemical Engineering, University of California, Berkeley, California, 94720

Tóm tắt

AbstractThe Production of cellulases and Hemicellulases was studied with Trichoderma reesei Rut C‐30, This organism produced, together with high cellulase activities, considerable amounts of xylanases and β‐glucosidase. Three cellulose concentration (1, 2.5, and 5.0%) were examined to determined the maximum levels of cellulase activity obtainable in submerged culture. Temperature and pH profiling was used to increase cell mass to maximum levels within two days and thereby enhancing fermentor productivity at higher substrate levels. The effect of temperature, pH, Tween‐80 concentration, carbon sources, and substrate concentration on the ration of mycelial growth and extracellulose enzyme production are described.

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Tài liệu tham khảo

Wilke C. R., 1975, Cellulose as a Chemical and Energy Resource, 253

Wilke C. R., 1975, Proceedings of the Symposium on Enzymatic Hydrolysis of Cellulose, 485

Wilke C. R., 1975, J. Appl. Polym. Sci. Symp., 28, 175

Nystrom J. M., 1976, Biotechnol. Bioeng. Symp., 6, 55

Nystrom J. M., 1975, Proceedings of the Symposium on Enzymatic Hydrolysis of Cellulose, 485

Anderotti R. E., 1977, Proceedings of the Bioconversion Symposium, 249

10.1002/bit.260181209

C. R.WilkeandH. W.Blanch Lawrence Berkekey Laboratory Berkeley California Report No. Wilke No. LBL‐8658 December 1978.

Montenecourt B. S., 1979, Adv. Chem. Ser., 181, 29

C. R.WilkeandH. W.Blanch Lawrence Berkeley Laboratory Berkeley California Report No. LBL‐9220 June 1 1979.

10.1021/ba-1969-0095.ch023

Mandels M., 1976, Biotechnol. Bioeng. Symp., 6, 55

10.1016/S0021-9258(19)52451-6

10.1002/abio.370010105

10.1271/bbb1961.33.1787

M.CastanonandC. R.Wilke Lawrence Berkeley Laboratory Berkeley California Report No. LBL‐5950 October 1976.

10.1021/ja01610a042

10.1002/jobm.3630020203

Nomura K., 1968, J. Ferment. Technol., 46, 634

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Thông tin xuất bản

Biotechnology and Bioengineering

Tập 23 Số 8

1837-1849

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