Enhanced membrane fluorescence of CDC-labelled Paramecium subsequent to removal of surface components

Springer Science and Business Media LLC - Tập 77 - Trang 171-175 - 1983
E. Wyroba1, G. Bottiroli2, P. Giordano2
1Department of Cell Biology, Nencki Institute of Experimental Biology, Warsaw, Poland
2Centro di Studio per l'Istochimica del C.N.R. and Istituto di Anatomia Comparata dell'Università, Pavia, Italy

Tóm tắt

Cytofluorimetric analysis of cycloheptaamylose-dansyl chloride (CDC) labelled Paramecium indicates that after mild trypsin removal of surface components the localization of CDC on the outer surface of living cells was not modified by the treatment. After such treatment the intensity of fluorescence emission was found about 3-fold higher in treated single cell than in the untreated one. These findings indicate that CDC labelling can be used to follow alteration occurred on the membrane of the living cell prior to labelling.

Tài liệu tham khảo

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