Enhanced membrane fluorescence of CDC-labelled Paramecium subsequent to removal of surface components
Tóm tắt
Cytofluorimetric analysis of cycloheptaamylose-dansyl chloride (CDC) labelled Paramecium indicates that after mild trypsin removal of surface components the localization of CDC on the outer surface of living cells was not modified by the treatment. After such treatment the intensity of fluorescence emission was found about 3-fold higher in treated single cell than in the untreated one. These findings indicate that CDC labelling can be used to follow alteration occurred on the membrane of the living cell prior to labelling.
Tài liệu tham khảo
Bottiroli G, Wyroba E, Giordano P (1981) Enhancement of fluorescence intensity of dansyl labelled Paramecium evoked by removal of surface components. Progr Protozool VI International Congress of Protozoology, Warsaw, p 36 (Abstr)
Chen RF (1967) Fluorescence of dansyl amino acids in organic solvents and protein solutions. Arch Biochem Biophys 120:609–620
Gahmberg GG, Hakomori SI (1974) Organization of glycolipids and glycoproteins in surface membranes: dependency on cell cycle and on transformation. Biochem Biophys Res Commun 59:283–291
Hawkes SP, Bartholomev JC (1977) Quantitative determination of transformed cells in a mixed population by simultaneous fluorescence analysis of cell surface and DNA in individual cells. Proc Natl Acad Sci USA 74:1626–1630
Hawkes SP, Meehan TD, Bissel MJ (1976) The use of fluorescamine as a probe for labelling the outer surface of the plasma membrane. Biochem Biophys Res Commun 68:1226–1233
Kinoshita T, Iinuma F, Tsuji A (1973) Enhancement of the fluorescence intensity of dansyl amino acids in aqueous media and its application to assay of amino acids. Biochem Biophys Res Commun 51:666–671
Nicolson GL (1976) Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over cell surface components. Biochim Biophys Acta 457:57–108
Reisner AH, Rowe J, Sleigh RW (1969) Concerning the tertiary structure of the soluble surface proteins of Paramecium. Biochemistry 8:4637–4644
Soldo AT, Godoy GA, van Wagtendonk WJ (1966) Growth of particle-bearing and particle-free Paramecium aurelia in axenic culture. J Protozool 13:492–497
Steers E Jr (1962) A comparison of the tryptic peptides obtained from immobilization antigens of Paramecium aurelia. Proc Natl Acad Sci USA 48:867–874
Wyroba E (1977) Studies on the surface coat of Paramecium aurelia. II. Relationship to the immobilization antigen. Cell Tissue Res 181:245–253
Wyroba E (1978) Cell multiplication following partial enzymatic removal of surface coat. Cytobiologie 17:412–420
Wyroba E (1980) Release of Paramecium immobilization antigen to the non-nutrient medium. Cell Biol Int Rep 4:1–10
Wyroba E, Bottiroli G, Giordano P (1981) Cycloheptaamylose-dansyl chloride complex as a fluorescent label of surface membranes in living ciliates. Histochemistry 73:459–467
Wyroba E, Brutkowska M (1978) Restoration of phagocytic activity of Paramecium tetraurelia suppressed by previous digestion of surface coat. Acta Protozool 17:515–524