Kỹ thuật chế tạo các hệ thống vận chuyển lipid nano thông minh được bao bọc bởi các hợp chất nano vàng và camptothecin cho điều trị hóa trị liệu và quang hóa trị liệu phối hợp trong ung thư buồng trứng

Yibing Fan1,2, Jiayu Wen1, Li Shi1, Yingqing Dai1
1Affiliated Changshu Hospital of Nantong University, Suzhou, China
2The Third Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, China

Tóm tắt

Ung thư buồng trứng mô tả một nhóm các rối loạn bắt đầu từ buồng trứng hoặc ống dẫn trứng và phúc mạc liền kề. Ngày càng rõ ràng rằng hóa trị liệu đơn độc không hiệu quả đối với ung thư buồng trứng. Để điều trị kết hợp hóa trị liệu/quang hóa trị liệu, chúng tôi đã hiệu quả phát triển các chất mang lipid nano cấu trúc (CPT + AuNCs@NSLCs) chứa camptothecin (CPT) và các hợp chất nano vàng (AuNCs). Các hợp chất nano vàng (AuNCs) với các hình dạng và kích thước khác nhau (thanh nano vàng [AuNRs] và cầu nano vàng [AuNSs]) đã được đưa vào NSLCs để tận dụng khả năng quang hóa của chúng và lý thuyết hóa sự kết hợp của các hệ thống vận chuyển thuốc cho điều trị hóa trị/quang hóa. Trước và sau khi được chiếu sáng bằng ánh sáng hồng ngoại gần (808 nm), NSLCs đã được kiểm tra khả năng giải phóng CPT một cách tự phát. AuNRs có tiềm năng làm nóng tối đa là ΔT = 22 °C trong 5 phút với 19.8 µg Au/mg lipid. Tế bào ung thư buồng trứng HeLa được sử dụng để đánh giá tác động độc tế bào của các công thức khác nhau, và dưới 5 phút phơi sáng NIR, CPT + AuNRs@NSLCs cho thấy sự ức chế sự phát triển đáng kể nhất (81%) trong nhóm. Đã được phát hiện rằng khả năng quang nhiệt của AuNCs bị ảnh hưởng trực tiếp bởi cấu trúc của chúng, rằng tính chất độc tế bào của CPT và hyperthermia cục bộ được cung cấp bởi AuNCs có thể gợi ý cải thiện thiệt hại tế bào, và rằng các nanoformulations tiên tiến đã được thiết lập rất hứa hẹn như một tác nhân quang hóa/hóa trị. Do đó, kết quả của nghiên cứu này cho thấy triển vọng trong việc chế tạo một nền tảng nano sinh học tương thích đa chức năng, được truyền lipid có thể áp dụng cho điều trị phối hợp và thuận lợi cho việc điều chỉnh các đặc tính của nó.

Từ khóa

#ung thư buồng trứng #hóa trị liệu #quang hóa trị liệu #camptothecin #hợp chất nano vàng #hệ thống vận chuyển thuốc #khả năng quang nhiệt #dòng tế bào HeLa

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