End-joining of blunt DNA double-strand breaks in mammalian fibroblasts is precise and requires DNA-PK and XRCC4

van Gent, 2001, Chromosomal stability and the DNA double-stranded break connection, Nat. Rev. Genet., 2, 196, 10.1038/35056049 Kanaar, 1998, Molecular mechanisms of DNA double strand break repair, Trends Cell Biol., 8, 483, 10.1016/S0962-8924(98)01383-X Karran, 2000, DNA double strand break repair in mammalian cells, Curr. Opin. Genet. Dev., 10, 144, 10.1016/S0959-437X(00)00069-1 Smith, 1999, The DNA-dependent protein kinase, Genes Dev., 13, 916, 10.1101/gad.13.8.916 Moshous, 2001, Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency, Cell, 105, 177, 10.1016/S0092-8674(01)00309-9 Ma, 2002, Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination, Cell, 108, 781, 10.1016/S0092-8674(02)00671-2 Rooney, 2002, Leaky Scid phenotype associated with defective V(D)J coding end processing in Artemis-deficient mice, Mol. Cell, 10, 1379, 10.1016/S1097-2765(02)00755-4 Ouyang, 1997, Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination in vivo, J. Exp. Med., 186, 921, 10.1084/jem.186.6.921 Nussenzweig, 1996, Requirement for Ku80 in growth and immunoglobulin V(D)J recombination, Nature, 382, 551, 10.1038/382551a0 Barnes, 1998, Targeted disruption of the gene encoding DNA ligase IV leads to lethality in embryonic mice, Curr. Biol., 8, 1395, 10.1016/S0960-9822(98)00021-9 Frank, 1998, Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV, Nature, 396, 173, 10.1038/24172 Gao, 1998, A targeted DNA-PKCS-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination, Immunity, 9, 367, 10.1016/S1074-7613(00)80619-6 Gu, 1997, Growth retardation and leaky SCID phenotype of Ku70-deficient mice, Immunity, 7, 653, 10.1016/S1074-7613(00)80386-6 Jhappan, 1997, DNA-PKCS: a T-cell tumour suppressor encoded at the mouse scid locus, Nat. Genet., 17, 483, 10.1038/ng1297-483 Zhu, 1996, Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates, Cell, 86, 379, 10.1016/S0092-8674(00)80111-7 Taccioli, 1998, Targeted disruption of the catalytic subunit of the DNA-PK gene in mice confers severe combined immunodeficiency and radiosensitivity, Immunity, 9, 355, 10.1016/S1074-7613(00)80618-4 Vogel, 1999, Deletion of Ku86 causes early onset of senescence in mice, Proc. Natl. Acad. Sci. U.S.A., 96, 10770, 10.1073/pnas.96.19.10770 Kabotyanski, 1998, Double-strand break repair in Ku86- and XRCC4-deficient cells, Nucleic Acids Res., 26, 5333, 10.1093/nar/26.23.5333 Verkaik, 2002, Different types of V(D)J recombination and end-joining defects in DNA double-strand break repair mutant mammalian cells, Eur. J. Immunol., 32, 701, 10.1002/1521-4141(200203)32:3<701::AID-IMMU701>3.0.CO;2-T Goryshin, 1998, Tn5 in vitro transposition, J. Biol. Chem., 273, 7367, 10.1074/jbc.273.13.7367 Zhou, 1998, Molecular genetic analysis of transposase-end DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn5 transposase, J. Mol. Biol., 276, 913, 10.1006/jmbi.1997.1579 Hesse, 1987, Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V-(D)-J joining signals, Cell, 49, 775, 10.1016/0092-8674(87)90615-5 Gao, 1998, A critical role for DNA end-joining proteins in both lymphogenesis and neurogenesis, Cell, 95, 891, 10.1016/S0092-8674(00)81714-6 Gu, 2000, Defective embryonic neurogenesis in Ku-deficient but not DNA-dependent protein kinase catalytic subunit-deficient mice, Proc. Natl. Acad. Sci. U.S.A., 97, 2668, 10.1073/pnas.97.6.2668 Lieber, 1988, The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination, Cell, 55, 7, 10.1016/0092-8674(88)90004-9 Errami, 1998, XR-C1, a new CHO cell mutant which is defective in DNA-PKCS, is impaired in both V(D)J coding and signal joint formation, Nucleic Acids Res., 26, 3146, 10.1093/nar/26.13.3146 Taccioli, 1994, A DNA repair defect in Chinese hamster ovary cells affects V(D)J recombination similarly to the murine scid mutation, J. Biol. Chem., 269, 7439, 10.1016/S0021-9258(17)37305-2 Lin, 1997, An ionizing radiation-sensitive CHO mutant cell line: irs-20. IV. Genetic complementation, V(D)J recombination and the scid phenotype, Radiat. Res., 147, 166, 10.2307/3579417 Shin, 1997, A kinase-negative mutation of DNA-PK(CS) in equine SCID results in defective coding and signal joint formation, J. Immunol., 158, 3565, 10.4049/jimmunol.158.8.3565 Pierce, 2001, Double-strand breaks and tumorigenesis, Trends Cell Biol., 11, S52, 10.1016/S0962-8924(01)82296-0 Liang, 1998, Homology-directed repair is a major double-strand break repair pathway in mammalian cells, Proc. Natl. Acad. Sci. U.S.A., 95, 5172, 10.1073/pnas.95.9.5172