Emerging roles for long noncoding RNAs in B-cell development and malignancy
Tài liệu tham khảo
Adams, 2017, Targeting noncoding RNAs in disease, J. Clin. Invest., 127, 761, 10.1172/JCI84424
Adriaens, 2016, p53 induces formation of NEAT1 lncRNA-containing paraspeckles that modulate replication stress response and chemosensitivity, Nat. Med., 22, 861, 10.1038/nm.4135
Bao, 2015, The p53-induced lincRNA-p21 derails somatic cell reprogramming by sustaining H3K9me3 and CpG methylation at pluripotency gene promoters, Cell Res., 25, 80, 10.1038/cr.2014.165
Bassig, 2015, Genetic susceptibility to diffuse large B-cell lymphoma in a pooled study of three Eastern Asian populations, Eur. J. Haematol., 95, 442, 10.1111/ejh.12513
Beck-Engeser, 2008, Pvt1-encoded microRNAs in oncogenesis, Retrovirology, 5, 10.1186/1742-4690-5-4
Berg, 2014, A transgenic mouse model demonstrating the oncogenic role of mutations in the polycomb-group gene EZH2 in lymphomagenesis, Blood, 123, 3914, 10.1182/blood-2012-12-473439
Blume, 2015, p53-dependent non-coding RNA networks in chronic lymphocytic leukemia, Leukemia, 29, 2015, 10.1038/leu.2015.119
Bonnal, 2015, De novo transcriptome profiling of highly purified human lymphocytes primary cells, Sci. Data, 2, 150051, 10.1038/sdata.2015.51
Brazao, 2016, Long noncoding RNAs in B-cell development and activation, Blood, 128, e10, 10.1182/blood-2015-11-680843
Cabili, 2011, Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses, Genes Dev., 25, 1915, 10.1101/gad.17446611
Cabili, 2015, Localization and abundance analysis of human lncRNAs at single-cell and single-molecule resolution, Genome Biol., 16, 10.1186/s13059-015-0586-4
Carramusa, 2007, The PVT-1 oncogene is a Myc protein target that is overexpressed in transformed cells, J. Cell. Physiol., 213, 511, 10.1002/jcp.21133
Casero, 2015, Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineages, Nat. Immunol., 16, 1282, 10.1038/ni.3299
Cerhan, 2014, Genome-wide association study identifies multiple susceptibility loci for diffuse large B cell lymphoma, Nat. Genet., 46, 1233, 10.1038/ng.3105
Cho, 2014, MALAT1 long non-coding RNA is overexpressed in multiple myeloma and may serve as a marker to predict disease progression, BMC Cancer, 14, 10.1186/1471-2407-14-809
Chu, 2012, Chromatin isolation by RNA purification (ChIRP), J. Vis. Exp., 3912
Clemson, 2009, An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles, Mol. Cell, 33, 717, 10.1016/j.molcel.2009.01.026
Colombo, 2015, PVT1: a rising star among oncogenic long noncoding RNAs, BioMed. Res. Int., 2015, 304208, 10.1155/2015/304208
Crowther-Swanepoel, 2010, Common variants at 2q37.3, 8q24.21, 15q21.3 and 16q24.1 influence chronic lymphocytic leukemia risk, Nat. Genet., 42, 132, 10.1038/ng.510
Cui, 2016, Long non-coding RNA PVT1 and cancer, Biochem. Biophys. Res. Commun., 471, 10, 10.1016/j.bbrc.2015.12.101
Dabovic, 2002, Bone abnormalities in latent TGF-[beta] binding protein (Ltbp)-3-null mice indicate a role for Ltbp-3 in modulating TGF-[beta] bioavailability, J. Cell Biol., 156, 227, 10.1083/jcb.200111080
Davis, 2015, Purification of specific chromatin regions using oligonucleotides: capture hybridization analysis of RNA targets (CHART), Methods Mol. Biol., 1262, 167, 10.1007/978-1-4939-2253-6_10
Derrien, 2012, The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression, Genome Res., 22, 1775, 10.1101/gr.132159.111
Diaz-Munoz, 2015, The RNA-binding protein HuR is essential for the B cell antibody response, Nat. Immunol., 16, 415, 10.1038/ni.3115
Dimitrova, 2014, LincRNA-p21 activates p21 in cis to promote Polycomb target gene expression and to enforce the G1/S checkpoint, Mol. Cell, 54, 777, 10.1016/j.molcel.2014.04.025
Doose, 2015, MINCR is a MYC-induced lncRNA able to modulate MYC’s transcriptional network in Burkitt lymphoma cells, Proc. Natl. Acad. Sci. U. S. A., 112, E5261, 10.1073/pnas.1505753112
Du, 2012, Elevation of highly up-regulated in liver cancer (HULC) by hepatitis B virus X protein promotes hepatoma cell proliferation via down-regulating p18, J. Biol. Chem., 287, 26302, 10.1074/jbc.M112.342113
Enciso-Mora, 2010, A genome-wide association study of Hodgkin’s lymphoma identifies new susceptibility loci at 2p16.1 (REL), 8q24.21 and 10p14 (GATA3), Nat. Genet., 42, 1126, 10.1038/ng.696
Enciso-Mora, 2010, A genome-wide association study of Hodgkin’s lymphoma identifies new susceptibility loci at 2p16.1 (REL), 8q24.21 and 10p14 (GATA3), Nat. Genet., 42, 1126, 10.1038/ng.696
Engreitz, 2014, RNA–RNA interactions enable specific targeting of noncoding RNAs to nascent Pre-mRNAs and chromatin sites, Cell, 159, 188, 10.1016/j.cell.2014.08.018
Esposti, 2016, Identification of novel long non-coding RNAs deregulated in hepatocellular carcinoma using RNA-sequencing, Oncotarget, 7, 31862, 10.18632/oncotarget.7364
Friesen, 1996, Involvement of the CD95 (APO-1/FAS) receptor/ligand system in drug-induced apoptosis in leukemia cells, Nat. Med., 2, 574, 10.1038/nm0596-574
Friesen, 1999, Cytotoxic drugs and the CD95 pathway, Leukemia, 13, 1854, 10.1038/sj.leu.2401333
Gardini, 2017, Global run-on sequencing (GRO-Seq), Methods Mol. Biol., 1468, 111, 10.1007/978-1-4939-4035-6_9
Guttman, 2009, Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals, Nature, 458, 223, 10.1038/nature07672
Hacisuleyman, 2014, Topological organization of multichromosomal regions by the long intergenic noncoding RNA Firre, Nat. Struct. Mol. Biol., 21, 198, 10.1038/nsmb.2764
Hart, 2014, MYC regulates the non-coding transcriptome, Oncotarget, 5, 12543, 10.18632/oncotarget.3033
Hart, 2016, MINCR is not a MYC-induced lncRNA, Proc. Natl. Acad. Sci. U. S. A., 113, E496, 10.1073/pnas.1519903113
Heinaniemi, 2016, Transcription-coupled genetic instability marks acute lymphoblastic leukemia structural variation hotspots, Elife, 5, 10.7554/eLife.13087
Hezroni, 2015, Principles of long noncoding RNA evolution derived from direct comparison of transcriptomes in 17 species, Cell Rep., 11, 1110, 10.1016/j.celrep.2015.04.023
Hu, 2014, The long non-coding RNA GAS5 cooperates with the eukaryotic translation initiation factor 4E to regulate c-Myc translation, PLoS One, 9, e107016, 10.1371/journal.pone.0107016
Huarte, 2010, A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response, Cell, 142, 409, 10.1016/j.cell.2010.06.040
Huppi, 2008, The identification of microRNAs in a genomically unstable region of human chromosome 8q24, Mol. Cancer Res., 6, 212, 10.1158/1541-7786.MCR-07-0105
Hutchinson, 2007, A screen for nuclear transcripts identifies two linked noncoding RNAs associated with SC35 splicing domains, BMC Genomics, 8, 39, 10.1186/1471-2164-8-39
Isin, 2014, Investigation of circulating lncRNAs in B-cell neoplasms, Clin. Chim. Acta, 431, 255, 10.1016/j.cca.2014.02.010
Iyer, 2015, The landscape of long noncoding RNAs in the human transcriptome, Nat. Genet., 47, 199, 10.1038/ng.3192
Ji, 2011, Cell-type independent MYC target genes reveal a primordial signature involved in biomass accumulation, PLoS One, 6, e26057, 10.1371/journal.pone.0026057
Karmali, 2017, Molecular subtyping in diffuse large B cell lymphoma: closer to an approach of precision therapy, Curr. Treat. Options Oncol., 18, 10.1007/s11864-017-0449-1
Kaur, 2013, MYC acts via the PTEN tumor suppressor to elicit autoregulation and genome-wide gene repression by activation of the Ezh2 methyltransferase, Cancer Res., 73, 695, 10.1158/0008-5472.CAN-12-2522
Khamas, 2012, Genome-wide screening for methylation-silenced genes in colorectal cancer, Int. J. Oncol., 41, 490, 10.3892/ijo.2012.1500
Kim, 2008, Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription, J. Biol. Chem., 283, 9113, 10.1074/jbc.M708205200
Kino, 2010, Noncoding RNA gas5 is a growth arrest- and starvation-associated repressor of the glucocorticoid receptor, Sci. Signal., 3, ra8, 10.1126/scisignal.2000568
Klein, 2011, Translocation-capture sequencing reveals the extent and nature of chromosomal rearrangements in B lymphocytes, Cell, 147, 95, 10.1016/j.cell.2011.07.048
Kwok, 2015, Synthetic lethality in chronic lymphocytic leukaemia with DNA damage response defects by targeting the ATR pathway, Lancet, 385
Li, 2014, Activation of LTBP3 gene by a long noncoding RNA (lncRNA) MALAT1 transcript in mesenchymal stem cells from multiple myeloma, J. Biol. Chem., 289, 29365, 10.1074/jbc.M114.572693
Li, 2017, The effects of the long non-coding RNA MALAT-1 regulated autophagy-related signaling pathway on chemotherapy resistance in diffuse large B-cell lymphoma, Biomed. Pharmacother., 89, 939, 10.1016/j.biopha.2017.02.011
Liang, 2014, Insulin-like growth factor 1 receptor is a prognostic factor in classical Hodgkin lymphoma, PLoS One, 9, e87474, 10.1371/journal.pone.0087474
Limon, 2012, Akt and mTOR in B cell activation and differentiation, Front. Immunol., 3, 228, 10.3389/fimmu.2012.00228
Lin, 2012, Transcriptional amplification in tumor cells with elevated c-Myc, Cell, 151, 56, 10.1016/j.cell.2012.08.026
Liu, 2013, Downregulation of GAS5 promotes bladder cancer cell proliferation, partly by regulating CDK6, PLoS One, 8, e73991, 10.1371/journal.pone.0073991
Lorenzin, 2016, Different promoter affinities account for specificity in MYC-dependent gene regulation, Elife, 5, 10.7554/eLife.15161
Leucci, 2016, Melanoma addiction to the long non-coding RNA SAMMSON, Nature, 531, 518, 10.1038/nature17161
Lu, 2013, Downregulation of gas5 increases pancreatic cancer cell proliferation by regulating CDK6, Cell Tissue Res., 354, 891, 10.1007/s00441-013-1711-x
Lu, 2015, Convergent BCL6 and lncRNA promoters demarcate the major breakpoint region for BCL6 translocations, Blood, 126, 1730, 10.1182/blood-2015-07-657999
Lund, 2014, EZH2 in normal and malignant hematopoiesis, Leukemia, 28, 44, 10.1038/leu.2013.288
Lunning, 2015, Mutation of chromatin modifiers; an emerging hallmark of germinal center B-cell lymphomas, Blood Cancer J., 5, e361, 10.1038/bcj.2015.89
Mao, 2011, Direct visualization of the co-transcriptional assembly of a nuclear body by noncoding RNAs, Nat. Cell Biol., 13, 95, 10.1038/ncb2140
Medina, 2010, OncomiR addiction in an in vivo model of microRNA-21-induced pre-B-cell lymphoma, Nature, 467, 86, 10.1038/nature09284
Menezes, 2015, A synthetic lethal screen reveals enhanced sensitivity to ATR inhibitor treatment in mantle cell lymphoma with ATM loss-of-function, Mol. Cancer Res., 13, 120, 10.1158/1541-7786.MCR-14-0240
Meng, 2014, Convergent transcription at intragenic super-enhancers targets AID-initiated genomic instability, Cell, 159, 1538, 10.1016/j.cell.2014.11.014
Meng, 2017, Long non-coding RNA CRNDE promotes multiple myeloma cell growth by suppressing miR-451, Oncol. Res., 25, 1207, 10.3727/096504017X14886679715637
Mihailovich, 2015, miR-17-92 fine-tunes MYC expression and function to ensure optimal B cell lymphoma growth, Nat. Commun., 6, 8725, 10.1038/ncomms9725
Morin, 2010, Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin, Nat. Genet., 42, 181, 10.1038/ng.518
Moumen, 2005, hnRNP K: an HDM2 target and transcriptional coactivator of p53 in response to DNA damage, Cell, 123, 1065, 10.1016/j.cell.2005.09.032
Mourtada-Maarabouni, 2014, Role of GAS5 noncoding RNA in mediating the effects of rapamycin and its analogues on mantle cell lymphoma cells, Clin. Lymphoma Myeloma Leuk., 14, 468, 10.1016/j.clml.2014.02.011
Mourtada-Maarabouni, 2008, Growth arrest in human T-cells is controlled by the non-coding RNA growth-arrest-specific transcript 5 (GAS5), J. Cell Sci., 121, 939, 10.1242/jcs.024646
Nagoshi, 2012, Frequent PVT1 rearrangement and novel chimeric genes PVT1-NBEA and PVT1-WWOX occur in multiple myeloma with 8q24 abnormality, Cancer Res., 72, 4954, 10.1158/0008-5472.CAN-12-0213
Nakamura, 2008, The GAS5 (growth arrest-specific transcript 5) gene fuses to BCL6 as a result of t(1;3)(q25;q27) in a patient with B-cell lymphoma, Cancer Genet. Cytogenet., 182, 144, 10.1016/j.cancergencyto.2008.01.013
Nie, 2012, c-Myc is a universal amplifier of expressed genes in lymphocytes and embryonic stem cells, Cell, 151, 68, 10.1016/j.cell.2012.08.033
Oh, 2014, Diffuse large B-cell lymphoma with histone H3 trimethylation at lysine 27: another poor prognostic phenotype independent of c-Myc/Bcl2 coexpression, Hum. Pathol., 45, 2043, 10.1016/j.humpath.2014.07.002
Oh, 2016, Long non-coding RNA HOTAIR expression in diffuse large B-cell lymphoma: in relation to polycomb repressive complex pathway proteins and H3K27 trimethylation, J. Pathol. Transl. Med., 50, 369, 10.4132/jptm.2016.06.06
Pedram Fatemi, 2015, Screening for small-molecule modulators of long noncoding RNA-protein interactions using alphaScreen, J. Biomol. Screen., 20, 1132, 10.1177/1087057115594187
Peng, 2016, Long noncoding RNA LUNAR1 associates with cell proliferation and predicts a poor prognosis in diffuse large B-cell lymphoma, Biomed. Pharmacother., 77, 65, 10.1016/j.biopha.2015.12.001
Peng, 2016, Long noncoding RNA HULC predicts poor clinical outcome and represents pro-oncogenic activity in diffuse large B-cell lymphoma, Biomed. Pharmacother., 79, 188, 10.1016/j.biopha.2016.02.032
Peng, 2017, LincRNA-p21 predicts favorable clinical outcome and impairs tumorigenesis in diffuse large B cell lymphoma patients treated with R-CHOP chemotherapy, Clin. Exp. Med., 17, 1, 10.1007/s10238-015-0396-8
Petri, 2015, Long noncoding RNA expression during human B-cell development, PLoS One, 10, e0138236, 10.1371/journal.pone.0138236
Pickard, 2015, Molecular and cellular mechanisms of action of tumour suppressor GAS5 LncRNA, Genes (Basel), 6, 484, 10.3390/genes6030484
Puente, 2011, Whole-genome sequencing identifies recurrent mutations in chronic lymphocytic leukaemia, Nature, 475, 101, 10.1038/nature10113
Qian, 2014, B cell super-enhancers and regulatory clusters recruit AID tumorigenic activity, Cell, 159, 1524, 10.1016/j.cell.2014.11.013
Ranzani, 2015, The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4, Nat. Immunol., 16, 318, 10.1038/ni.3093
Rinn, 2007, Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs, Cell, 129, 1311, 10.1016/j.cell.2007.05.022
Salmena, 2011, A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language, Cell, 146, 353, 10.1016/j.cell.2011.07.014
Sattari, 2016, Upregulation of long noncoding RNA MIAT in aggressive form of chronic lymphocytic leukemias, Oncotarget, 7, 54174, 10.18632/oncotarget.11099
Sauvageau, 2013, Multiple knockout mouse models reveal lincRNAs are required for life and brain development, Elife, 2, e01749, 10.7554/eLife.01749
Schmitt, 2016, Long Noncoding RNAs in Cancer Pathways, Cancer Cell., 29, 452, 10.1016/j.ccell.2016.03.010
Schneider, 1988, Genes specifically expressed at growth arrest of mammalian cells, Cell, 54, 787, 10.1016/S0092-8674(88)91065-3
Sehgal, 2014, FAS-antisense 1 lncRNA and production of soluble versus membrane Fas in B-cell lymphoma, Leukemia, 28, 2376, 10.1038/leu.2014.126
Shao, 2016, Highly expressed lncRNA CRNDE promotes cell proliferation through Wnt/beta-catenin signaling in renal cell carcinoma, Tumour Biol., 12, 15997, 10.1007/s13277-016-5440-0
Shi, 2015, A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer, Mol. Carcinog., 54, E1, 10.1002/mc.22120
Skibola, 2014, Genome-wide association study identifies five susceptibility loci for follicular lymphoma outside the HLA region, Am. J. Hum. Genet., 95, 462, 10.1016/j.ajhg.2014.09.004
Smith, 1998, Classification of gas5 as a multi-small-nucleolar-RNA (snoRNA) host gene and a member of the 5’-terminal oligopyrimidine gene family reveals common features of snoRNA host genes, Mol. Cell. Biol., 18, 6897, 10.1128/MCB.18.12.6897
Song, 2016, Long non-coding RNA CRNDE promotes tumor growth in medulloblastoma, Eur. Rev. Med. Pharmacol. Sci., 20, 2588
Stromberg, 2015, Picropodophyllin inhibits proliferation and survival of diffuse large B-cell lymphoma cells, Med. Oncol., 32, 10.1007/s12032-015-0630-y
Sun, 2016, A potential panel of six-long non-coding RNA signature to improve survival prediction of diffuse large-B-cell lymphoma, Sci. Rep., 6, 27842, 10.1038/srep27842
Tayari, 2016, Long noncoding RNA expression profiling in normal B-cell subsets and hodgkin lymphoma reveals hodgkin and reed-sternberg cell-specific long noncoding RNAs, Am. J. Pathol., 186, 2462, 10.1016/j.ajpath.2016.05.011
Trimarchi, 2014, Genome-wide mapping and characterization of Notch-regulated long noncoding RNAs in acute leukemia, Cell, 158, 593, 10.1016/j.cell.2014.05.049
Tseng, 2014, PVT1 dependence in cancer with MYC copy-number increase, Nature, 512, 82, 10.1038/nature13311
Tsutsumi, 2013, Deletion or methylation of CDKN2A/2B and PVT1 rearrangement occur frequently in highly aggressive B-cell lymphomas harboring 8q24 abnormality, Leuk. Lymphoma, 54, 2760, 10.3109/10428194.2013.790543
Ulitsky, 2016, Evolution to the rescue: using comparative genomics to understand long non-coding RNAs, Nat. Rev. Genet., 17, 601, 10.1038/nrg.2016.85
Verma, 2015, Transcriptome sequencing reveals thousands of novel long non-coding RNAs in B cell lymphoma, Genome Med., 7, 10.1186/s13073-015-0230-7
Volders, 2013, LNCipedia: a database for annotated human lncRNA transcript sequences and structures, Nucleic Acids Res., 41, D246, 10.1093/nar/gks915
Wang, 2014, Oncofetal long noncoding RNA PVT1 promotes proliferation and stem cell-like property of hepatocellular carcinoma cells by stabilizing NOP2, Hepatology, 60, 1278, 10.1002/hep.27239
Wang, 2016, LncRNA MALAT1 promotes development of mantle cell lymphoma by associating with EZH2, J. Transl. Med., 14, 10.1186/s12967-016-1100-9
Wang, 2016, The long noncoding RNA MALAT-1 is a novel biomarker in various cancers: a meta-analysis based on the GEO database and literature, J. Cancer, 7, 991, 10.7150/jca.14663
Wein, 2015, Potential role of hypoxia in early stages of Hodgkin lymphoma pathogenesis, Haematologica, 100, 1320, 10.3324/haematol.2015.127498
Willander, 2013, NOTCH1 mutations influence survival in chronic lymphocytic leukemia patients, BMC Cancer, 13, 10.1186/1471-2407-13-274
Winkle, 2015, Long noncoding RNAs as a novel component of the Myc transcriptional network, FASEB J., 29, 2338, 10.1096/fj.14-263889
Wu, 2016, LncRNA-HOTAIR promotes TNF-alpha production in cardiomyocytes of LPS-induced sepsis mice by activating NF-kappaB pathway, Biochem. Biophys. Res. Commun., 471, 240, 10.1016/j.bbrc.2016.01.117
Xing, 2014, lncRNA directs cooperative epigenetic regulation downstream of chemokine signals, Cell, 159, 1110, 10.1016/j.cell.2014.10.013
Xu, 2012, mTOR, linking metabolism and immunity, Semin. Immunol., 24, 429, 10.1016/j.smim.2012.12.005
Yan, 2016, Elevated RNA. expression of long noncoding HOTAIR promotes cell proliferation and predicts a poor prognosis in patients with diffuse large B cell lymphoma, Mol. Med. Rep., 13, 5125, 10.3892/mmr.2016.5190
Yang, 2014, TGF-beta upregulates CD70 expression and induces exhaustion of effector memory T cells in B-cell non-Hodgkin’s lymphoma, Leukemia, 28, 1872, 10.1038/leu.2014.84
Yap, 2011, Somatic mutations at EZH2 Y641 act dominantly through a mechanism of selectively altered PRC2 catalytic activity, to increase H3K27 trimethylation, Blood, 117, 2451, 10.1182/blood-2010-11-321208
Yoshimoto, 2016, MALAT1 long non-coding RNA in cancer, Biochim. Biophys. Acta, 1859, 192, 10.1016/j.bbagrm.2015.09.012
Zeidler, 1994, Breakpoints of Burkitt’s lymphoma t(8;22) translocations map within a distance of 300kb downstream of MYC, Genes Chromosomes Cancer, 9, 282, 10.1002/gcc.2870090408
Zhang, 2013, Negative regulation of lncRNA GAS5 by miR-21, Cell Death Differ., 20, 1558, 10.1038/cdd.2013.110
Zhao, 2015, LncRNA HULC affects the differentiation of Treg in HBV-related liver cirrhosis, Int. Immunopharmacol., 28, 901, 10.1016/j.intimp.2015.04.028
Zhou, 2015, Strong expression of EZH2 and accumulation of trimethylated H3K27 in diffuse large B-cell lymphoma independent of cell of origin and EZH2 codon 641 mutation, Leuk. Lymphoma, 56, 2895, 10.3109/10428194.2015.1006220
Zhou, 2017, Discovery and validation of immune-associated long non-coding RNA biomarkers associated with clinically molecular subtype and prognosis in diffuse large B cell lymphoma, Mol. Cancer, 16, 10.1186/s12943-017-0580-4
Zhu, 2016, Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library, Nat. Biotechnol., 34, 1279, 10.1038/nbt.3715