Elucidation of the liver proteome in response to an antioxidant intake in rabbits

Springer Science and Business Media LLC - 2021
Akeem Babatunde Sikiru1, A. Arunachalam2, S.S.A. Egena1, Veerasamy Sejian3, I. J. Reddy3, Raghavendra Bhatta4
1Department of Animal Production, Federal University of Technology, Minna, 920262, Nigeria
2Reproductive Physiology Laboratory, ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, 560030, India
3Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, 560030, India
4ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, 560030, India

Tóm tắt

AbstractBackgroundAntioxidant intakes are one of the most cherished dietary approaches for the management of oxidative stress-induced liver damages. These antioxidants exist as the bioactive compounds present in plants and other natural sources functioning in varieties of ways from acting as direct scavengers of the free radicals to acting as the modifiers of genes and proteins expressions.Chlorella vulgarisis one of such antioxidants; it is a unicellular microalga and a rich source of polyphenols which has been reported for its capacity of reducing oxidative stress by upregulation of antioxidant genes. However, there are scarce reports on its effect on antioxidant protein expressions and functions in the liver. This situation necessitates untargeted proteomic profiling of the liver due to the antioxidant intakes as carried out in this present study. Sixteen laboratory weaner rabbits of 8 weeks old with initial average bodyweight of 1060 ± 29.42 g were randomly divided into two groups (n= 8 per group); the first group served as control while the second served as the treatment group were used for this study.ResultsAfter a period of 120 days daily consumption of 500 mg ofChlorella vulgarisbiomass per kg bodyweight of the rabbit models, the animals were sacrificed and their livers were harvested followed by protein extraction for the untargeted proteomic profiling using LC-MS/Orbitrap Fusion Tribrid™ peptides quantifier and sequencer. Also, there was an assessment of the oxidative stress biomarkers in the liver and serum of the rabbits. Five-hundred and forty-four (544) proteins were identified out of which 204 were unique to the control, 198 were unique to the treatment group, while 142 were common to both groups of the rabbits. Antioxidant proteins commonly found in both groups were upregulated in the treatment group and were significantly associated with oxidative stress-protective activities. There was a reduction in oxidative stress biomarkers of the supplemented group as indicated by the assessment of the liver malondialdehyde concentrations (p< 0.05), total antioxidant capacities (p< 0.05), and antioxidant enzyme activities (p< 0.05). Similarly, these biomarkers were significantly reduced in the serum of the supplemented rabbits (p< 0.05).ConclusionThe study concluded thatChlorella vulgarisis an antioxidant agent that could be suitable for reducing liver oxidative stress damage and it is a potential drug candidate for protecting the liver against oxidative stress damages as revealed in the rabbit models.

Từ khóa


Tài liệu tham khảo

Li S, Tan H-Y, Wang N, Zhang Z-J, Lao L, Wong C-W, Feng Y (2015) The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci 16(11):26087–26124. https://doi.org/10.3390/ijms161125942

Farzaei M, Zobeiri M, Parvizi F, El-Senduny F, Marmouzi I, Coy-Barrera E et al (2018) Curcumin in liver diseases: a systematic review of the cellular mechanisms of oxidative stress and clinical perspective. Nutrients 10(7):855. https://doi.org/10.3390/nu10070855

Agarwal A, Roychoudhury S, Bjugstad KB, Cho C-L (2016) Oxidation-reduction potential of semen: what is its role in the treatment of male infertility? Ther Adv Urol 8(5):302–318. https://doi.org/10.1177/1756287216652779

Christaki E, Karatzia M, Florou-Paneri P (2010) The use of algae in animal nutrition. J Hell Vet Med Soc 61:267–276

Sikiru AB, Arangasamy A, Alemede IC, Guvvala PR, Egena SSA, Ippala JR, Bhatta R (2019) Chlorella vulgaris supplementation effects on performances, oxidative stress and antioxidant genes expression in liver and ovaries of New Zealand White rabbits. Heliyon 5(9):e02470. https://doi.org/10.1016/j.heliyon.2019.e02470

Kausar S, Wang F, Cui H (2018) The role of mitochondria in reactive oxygen species generation and its implications for neurodegenerative diseases. Cells 7(12):274. https://doi.org/10.3390/cells7120274

Fujii J, Ikeda Y (2002) Advances in our understanding of peroxiredoxin, a multifunctional, mammalian redox protein. Redox Rep 7(3):123–130. https://doi.org/10.1179/135100002125000352

Asrani SK, Devarbhavi H, Eaton J, Kamath PS (2019) Burden of liver diseases in the world. J Hepatol 70(1):151–171. https://doi.org/10.1016/j.jhep.2018.09.014

Hong M, Li S, Tan H, Wang N, Tsao S-W, Feng Y (2015) Current status of herbal medicines in chronic liver disease therapy: the biological effects, molecular targets and future prospects. Int J Mol Sci 16(12):28705–28745. https://doi.org/10.3390/ijms161226126

Sikiru A, Arangasamy A, Alemede I, Egena S, Ippala J, Bhatta R (2021) Effects of dietary supplementation of Chlorella vulgaris on oxidative stress attenuation and serum biochemical profile of pregnant New Zealand White rabbits. Indian J Anim Sci 90:88–91

Sikiru AB, Arangasamy A, Alemede IC, Egena SSA, Bhatta R (2019) Dietary supplementation effects of Chlorella vulgaris on performances, oxidative stress status and antioxidant enzymes activities of prepubertal New Zealand White rabbits. Bull Natl Res Cent 43(1). https://doi.org/10.1186/s42269-019-0213-8

Sikiru A, Arangasamy A, Ijaiya A, Ippala R, Bhatta R (2019) Effects of Chlorella vulgaris supplementation on performances of lactating nulliparous New Zealand white rabbits does and their kits. Int J Livest Res 1. https://doi.org/10.5455/ijlr.20190626094302

Pike TW, Blount JD, Metcalfe NB, Lindström J (2010) Dietary carotenoid availability and reproductive effort influence the age-related decline in performance. Behav Ecol 21(5):1048–1053. https://doi.org/10.1093/beheco/arq102

Kurutas EB (2015) The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J 15(1):71. https://doi.org/10.1186/s12937-016-0186-5

Butterfield D, Perluigi M (2017) Redox proteomics: a key tool for new insights into protein modification with relevance to disease

Kozlowski LP (2016) IPC – Isoelectric Point Calculator. Biol Direct 11(1):55. https://doi.org/10.1186/s13062-016-0159-9

Raudvere U, Kolberg L, Kuzmin I, Arak T, Adler P, Peterson H et al (2019) g:Profiler: a web server for functional enrichment analysis and conversions of gene lists. Nucleic Acids Res 47:191–198

Kalyanaraman B, Cheng G, Hardy M, Ouari O, Bennett B, Zielonka J (2018) Teaching the basics of reactive oxygen species and their relevance to cancer biology: mitochondrial reactive oxygen species detection, redox signaling, and targeted therapies. Redox Biol 15:347–362. https://doi.org/10.1016/j.redox.2017.12.012

Mennerich D, Kellokumpu S, Kietzmann T (2019) Hypoxia and reactive oxygen species as modulators of endoplasmic reticulum and golgi homeostasis. Antioxid Redox Signal 30(1):113–137. https://doi.org/10.1089/ars.2018.7523

Baker MA, Netherton J, Aitken RJ (2019) From past to present. oxid. Antioxidants impact Oxidative Status Male Reprod. Elsevier, pp 17–26. https://doi.org/10.1016/B978-0-12-812501-4.00003-1

Tejero J, Shiva S, Gladwin MT (2019) Sources of vascular nitric oxide and reactive oxygen species and their regulation. Physiol Rev 99(1):311–379. https://doi.org/10.1152/physrev.00036.2017

Valavanidis A (2019) Oxidative stress and pulmonary carcinogenesis through mechanisms of reactive oxygen species. How respirable particulate matter, fibrous dusts, and ozone cause pulmonary inflammation and initiate lung carcinogenesis. In: Oxidative Stress Lung Dis. Springer Singapore, Singapore, pp 247–265. https://doi.org/10.1007/978-981-13-8413-4_13

Han E-S, Muller FL, Pérez VI, Qi W, Liang H, Xi L, Fu C, Doyle E, Hickey M, Cornell J, Epstein CJ, Roberts LJ, van Remmen H, Richardson A (2008) The in vivo gene expression signature of oxidative stress. Physiol Genomics 34(1):112–126. https://doi.org/10.1152/physiolgenomics.00239.2007

Ahmed AA, Fedail JS, Musa HH, Musa TH, Sifaldin AZ (2016) Gum Arabic supplementation improved antioxidant status and alters expression of oxidative stress gene in ovary of mice fed high fat diet. Middle East Fertil Soc J 21(2):101–108. https://doi.org/10.1016/j.mefs.2015.10.001

Dornas WC, Cardoso LM, Silva M, Machado NLS, Chianca DA, Alzamora AC, Lima WG, Lagente V, Silva ME (2017) Oxidative stress causes hypertension and activation of nuclear factor-κB after high-fructose and salt treatments. Sci Rep 7(1):46051. https://doi.org/10.1038/srep46051

Song X, Narzt MS, Nagelreiter IM, Hohensinner P, Terlecki-Zaniewicz L, Tschachler E, Grillari J, Gruber F (2017) Autophagy deficient keratinocytes display increased DNA damage, senescence and aberrant lipid composition after oxidative stress in vitro and in vivo. Redox Biol 11:219–230. https://doi.org/10.1016/j.redox.2016.12.015

Barber S, Mead R, Shaw P (2006) Oxidative stress in ALS: a mechanism of neurodegeneration and a therapeutic target. Biochim Biophys Acta (BBA)-Molecular Basis Dis 1762:1051–1067

Halliwell B, Gutteridge J (2015) Free radicals in biology and medicine. https://doi.org/10.1093/acprof:oso/9780198717478.001.0001

Perl A (2013) Oxidative stress in the pathology and treatment of systemic lupus erythematosus. Nat Rev Rheumatol 9(11):674–686. https://doi.org/10.1038/nrrheum.2013.147

Manevich Y, Fisher A (2005) Peroxiredoxin 6, a 1-Cys peroxiredoxin, functions in antioxidant defense and lung phospholipid metabolism. Free Radic Biol Med 38(11):1422–1432. https://doi.org/10.1016/j.freeradbiomed.2005.02.011

Park M (2018) Dissecting the impact of macrophage migration inhibitory factor (MIF) on host immune response

Ayala A, Muñoz M, Argüelles S (2014) Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Med Cell Longev 2014:1–31. https://doi.org/10.1155/2014/360438

Gudipaty S, Conner C, Rosenblatt J, Montell D (2018) Unconventional ways to live and die: cell death and survival in development, homeostasis, and disease. Annu Rev Cell Dev Biol 34(1):311–332. https://doi.org/10.1146/annurev-cellbio-100616-060748

Ramos-Tovar E, Muriel P (2020) Molecular mechanisms that link oxidative stress, inflammation, and fibrosis in the liver. Antioxidants 9(12):1279. https://doi.org/10.3390/antiox9121279

Ebrahimi-Mameghani M, Aliashrafi S, Javadzadeh Y, AsghariJafarabadi M (2014) The effect of chlorella vulgaris supplementation on liver en-zymes, serum glucose and lipid profile in patients with non-alcoholic fatty liver disease. Health Promot Perspect 4(1):107–115. https://doi.org/10.5681/hpp.2014.014

Pluchino N, Russo M, Santoro A, Litta P, Cela V, Genazzani A (2013) Steroid hormones and BDNF. Neuroscience 239:271–279. https://doi.org/10.1016/j.neuroscience.2013.01.025

Thông tin
Thông tin xuất bản

Springer Science and Business Media LLC

Thông tin tác giả