Elimination of virus and rapid propagation of disease-free sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture
Tóm tắt
The use of apical meristem culture for simultaneous virus elimination and shoot proliferation in sugarcane was assessed. Virus-free plants were propagated from Sugarcane mosaic virus and Sugarcane yellow leaf virus-infected material of the South African commercial cultivar, NCo376. A combination of thermotherapy by hot water treatment of stem sections (nodes) and subsequent germination of vegetative buds at 40°C and optimal meristem size were key factors for the production of virus-free plants. Only meristems of 2 mm in length or of a smaller size (but >0.5 mm) resulted in virus-free sugarcane. Shoot induction and proliferation via direct organogenesis were achieved on Murashige and Skoog nutrient medium supplemented with 0.1 mg l−1 6-benzyladenine and 0.015 mg l−1 6-furfurylaminopurine (KIN). The established protocol provides for the rapid proliferation of virus-free shoots from infected sugarcane plants and approximately 1,300 shoots were propagated from a single 2 mm meristem in 11 weeks. Plants remained virus-free when tested 12 months later.
Tài liệu tham khảo
Alegria OM, Royer M, Bousalem M, Chatenet M, Petershmitt M, Girad JC, Rott P (2003) Genetic diversity in the coat protein coding region of eighty-six sugarcane mosaic virus isolates from eight countries, particularly Cameroon and Congo. Arch Virol 148:357–372
Ali A, Naz S, Siddiqui FA, Iqbal J (2008) An efficient protocol for large scale production of sugarcane through micropropagation. Pak J Bot 40:139–149
Bajaj YPS, Jian LC (1995) Cryopreservation of sugarcane (Saccharum spp.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 32. Springer, Berlin, pp 256–265
Baksha R, Alam R, Karim MZ, Paul SK, Hossain MA, Miah MAS, Rahman ABMM (2002) In vitro shoot tip culture of sugar-cane (Saccharum Officinarum) variety Isd 28. Biotechnology 1:67–72
Balamuralikrishnan M, Dorisamy S, Ganapathy T, Viswanathan R (2002) Combined effect of chemotherapy and meristem culture on sugarcane mosaic virus elimination in sugarcane. Sugar Tech 4:19–25
Brumbley SM, Snyman SJ, Gnanasambandam A, Joyce P, Hermann SR, da Silva JAG, McQualter RB, Wang M, Egan BT, Patterson AH, Albert HH, Moore PH (2008) Sugarcane. In: Kole C, Hall TC (eds) A compendium of transgenic crop plants, vol 7: transgenic sugar, tuber and fibre crops. Blackwell, Oxford, pp 1–58
Burner DM, Grisham MP (1995) Induction and stability of phenotypic variation in sugarcane as affected by propagation procedure. Crop Sci 35:875–880
Chatenet M, Delage C, Ripolles M, Irey M, Lockhart BEL, Rott P (2001) Detection of sugarcane yellow leaf virus in quarantine and production of virus-free sugarcane by apical meristem culture. Plant Dis 85:1177–1180
Cha-um S, Hien NT, Kirdmanee C (2006) Disease free production of sugarcane varieties (Saccharum officinarum L.) using in vitro meristem culture. Biotechnology 5:443–448
Fitch MMM, Lehrer AT, Komor E, Moore PH (2001) Elimination of sugarcane yellow leaf virus from infected sugarcane plants by meristem tip culture visualized by tissue blot immunoassay. Plant Pathol 50:676–680
Flynn J, Powell G, Perdomo R, Montes G, Quebedeaux K, Comstock J (2005) Comparison of sugarcane disease incidence and yield of field-run, heat-treated and tissue-culture based seed cane. J Am Soc Sug Technol 25:88–100
Grout BWW (1990) Meristem-tip culture for propagation and virus elimination. In: Hall RD (ed) Methods in molecular biology, vol 111 plant cell culture protocols. Humana Press Inc, Totowa, New Jersey, pp 115–123
Kane ME (2005) Shoot culture procedures. In: Trigiano RN, Gray DJ (eds) Plant development and biotechnology. CRC Press LLC, Boca Raton, pp 145–157
LMC International (2008) Sugar and sweetners quarterly. Fourth Quarter, Oxford, England, pp 1–20. http://www.lmc.co.uk
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Parmessur Y, Aljanabi S, Saumtally S, Dookun-Saumtally A (2002) Sugarcane yellow leaf virus and sugarcane yellows phytoplasma: elimination by tissue-culture. Plant Pathol 51:561–566
Rott P, Mirkov TE, Schenk S, Girard J-C (2000) Recent advances in research on sugarcane yellow leaf virus, the causal agent of sugarcane yellow leaf. Proc Int Soc Sugar Cane Technol 26:968–977
Victoria JI, Guzmán ML, Garcés F, Jaramillo AD (1999) Pathogen-free seedcane production and its impact on a commercial scale in Colombia. In: Singh V, Kumar V (eds) Proceedings of the XXIII ISSCT Congress, ISSCT Congress Organising Committee, STAI, New Delhi, India, pp 390–396
Visessuwan R, Korpraditskul W, Attathom S, Klinkong S (1988) Production of virus free sugarcane by tissue culture. In: Kasetsart J (ed) Nat Sci Suppl 22:30–36
Visessuwan R, Chiemsombat P, Chanprame S, Naritoom K, Kuhapitaktharm R (1991) Detection of sugarcane mosaic virus in sugarcane plantlets derived from meristem culture. In: Kasetsart J (ed) Nat Sci Suppl 25:1–4
Visessuwan R, Chiemsombat P, Naritoom K, Surijachaijakorn M (1999) Role of growth regulators in meristem culture and production of virus free sugarcane germplasm. Sugar Tech 1:82–88
Wang Q, Cuellar WJ, Rajamäki M, Hirata Y, Valkonen JPR (2007) Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Mol Plant Pathol 8:1–14
Zhang M-Q, Rao GP, Gaur RK, Ruan M-H, Singh M, Sharma SR, Singh A, Singh P (2006) Sugarcane mosaic virus. In: Rao GP, Kharuna P, Lenardon SL (eds) Characterisation, diagnosis and management of plant viruses vol 1. Industrial crops. Stadium Press, Texas, pp 111–144