Electrospun Gelatin Fibers with a Multiple Release of Antibiotics Accelerate Dermal Regeneration in Infected Deep Burns

Macromolecular Bioscience - Tập 16 Số 9 - Trang 1368-1380 - 2016
Jianmei Chen1,2, Zongguang Liu1,3, Maohua Chen1, Hong Zhang1, Xiaohong Li1
1Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, P.R. China
2The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003 P. R. China
3School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, P. R. China

Tóm tắt

Electrospun fibers of hydrophilic polymers meet challenges in a rapid degradation of fiber matrices and discharge of antibiotics to comply with requirements of infection control as a dermal regeneration template. In the current study, a pH conversion process is initially developed to ensure fluent electrospinning, an efficient in situ cross‐linking of electrospun gelatin fibers with oxidized alginate and simultaneous loading of gentamicin sulfate (GS) and hydrophobic ciprofloxacin into fibers. The dual drug‐loaded fibers indicate a complete release of GS during 6 d and a sustained release of ciprofloxacin for over three weeks, and the antibiotics release indicates significant growth inhibitions on Pseudomonas aeruginosa and Staphylococcus epidermidis. The wound healing efficacy is evaluated on a deep burn model infected with 108 CFU of P. aeruginosa. Compared with fibers with loaded individual drugs, the concomitant release of GS and ciprofloxacin significantly reduces the bacteria numbers in wound and livers, at around 2.30 × 105 and 1.25 × 103 CFU after 3 d, respectively. The wound re‐epithelization, blood vessel formation, collagen deposition, and tissue remodeling process are accelerated with a complete healing observed after 21 d. This study provides a feasible strategy to design cross‐linked hydrophilic fibers with an extended drug release for biomedical applications. image

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Thông tin xuất bản

Macromolecular Bioscience

Tập 16 Số 9

1368-1380

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