Electroporation of outer membrane vesicles derived from Pseudomonas aeruginosa with gold nanoparticles
Tóm tắt
Since their discovery, extracellular vesicles have gained considerable scientific interest as a novel drug delivery system. In particular, outer membrane vesicles (OMVs) play a critical role in bacteria–bacteria communication and bacteria–host interactions by trafficking cell signalling biochemicals (i.e. DNA, RNA, proteins). Although previous studies have focused on the use of OMVs as vaccines, little work has been done on loading them with functional nanomaterials for drug delivery. We have developed a novel drug delivery system by loading OMVs with gold nanoparticles (AuNPs). AuNPs are versatile nanoparticles that have been extensively used in disease therapeutics. The particles were loaded into the vesicles via electroporation, which uses an electric pulse to create a short-lived electric field. The resulting capacitance on the membrane generates pores in the lipid bilayer of the OMVs allowing AuNPs (or any nanoparticle under 10 nm) inside the vesicles. Closure of the pores of the lipid membrane of the OMVs entraps the nanoparticles as cargo. Transmission electron microscopy was used to confirm the loading of AuNPs inside the OMVs and dynamic light scattering (DLS) and cryogenic scanning electron microscopy (cryo-SEM) verified the size and integrity of the OMVs. This is the first report to load nanoparticles into OMVs, demonstrating a potential method for drug delivery.
Tài liệu tham khảo
Kakkar A, Traverso G, Farokhzad OC et al (2017) Evolution of macromolecular complexity in drug delivery systems. Nat Rev Chem 1:0063. https://doi.org/10.1038/s41570-017-0063
Derfus AM, Chen AA, Min D-H et al (2007) Targeted quantum dot conjugates for siRNA delivery. Bioconjug Chem 18:1391–1396. https://doi.org/10.1021/BC060367E
Wang F, Wang Y-C, Dou S et al (2011) Doxorubicin-tethered responsive gold nanoparticles facilitate intracellular drug delivery for overcoming multidrug resistance in cancer cells. ACS Nano 5:3679–3692. https://doi.org/10.1021/nn200007z
Ménard-Moyon C, Venturelli E, Fabbro C et al (2010) The alluring potential of functionalized carbon nanotubes in drug discovery. Expert Opin Drug Discov 5:691–707. https://doi.org/10.1517/17460441.2010.490552
Wang Q, Zhuang X, Mu J et al (2013) Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids. Nat Commun 4:1867. https://doi.org/10.1038/ncomms2886
de la Torre Gomez C, Goreham RV, Bech Serra JJ et al (2018) “Exosomics”—a review of biophysics, biology and biochemistry of exosomes with a focus on human breast milk. Front Genet 9:92. https://doi.org/10.3389/fgene.2018.00092
Dobhal G, Ayupova D, Laufersky G et al (2018) Cadmium-free quantum dots as fluorescent labels for exosomes. Sensors 18:3308. https://doi.org/10.3390/s18103308
Tian T, Zhang H-X, He C-P et al (2018) Surface functionalized exosomes as targeted drug delivery vehicles for cerebral ischemia therapy. Biomaterials 150:137–149. https://doi.org/10.1016/j.biomaterials.2017.10.012
Gorringe A, Halliwell D, Matheson M et al (2005) The development of a meningococcal disease vaccine based on Neisseria lactamica outer membrane vesicles. Vaccine 23:2210–2213. https://doi.org/10.1016/j.vaccine.2005.01.055
Sun D, Zhuang X, Xiang X et al (2010) A novel nanoparticle drug delivery system: the anti-inflammatory activity of curcumin is enhanced when encapsulated in exosomes. Mol Ther 18:1606–1614. https://doi.org/10.1038/mt.2010.105
Zhuang X, Xiang X, Grizzle W et al (2011) Treatment of brain inflammatory diseases by delivering exosome encapsulated anti-inflammatory drugs from the nasal region to the brain. Mol Ther 19:1769–1779. https://doi.org/10.1038/mt.2011.164
Alvarez-Erviti L, Seow Y, Yin H et al (2011) Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol 29:341–345. https://doi.org/10.1038/nbt.1807
Shtam TA, Kovalev RA, Varfolomeeva E et al (2013) Exosomes are natural carriers of exogenous siRNA to human cells in vitro. Cell Commun Signal 11:88. https://doi.org/10.1186/1478-811X-11-88
Wahlgren J, Karlson TDL, Brisslert M et al (2012) Plasma exosomes can deliver exogenous short interfering RNA to monocytes and lymphocytes. Nucleic Acids Res 40:e130–e130. https://doi.org/10.1093/nar/gks463
Tian Y, Li S, Song J et al (2014) A doxorubicin delivery platform using engineered natural membrane vesicle exosomes for targeted tumor therapy. Biomaterials 35:2383–2390. https://doi.org/10.1016/j.biomaterials.2013.11.083
Zhao J-Y, Chen G, Gu Y-P et al (2016) Ultrasmall magnetically engineered Ag2 Se quantum dots for instant efficient labeling and whole-body high-resolution multimodal real-time tracking of cell-derived microvesicles. J Am Chem Soc 138:1893–1903. https://doi.org/10.1021/jacs.5b10340
Hood JL, Scott MJ, Wickline SA (2014) Maximizing exosome colloidal stability following electroporation. Anal Biochem 448:41–49. https://doi.org/10.1016/j.ab.2013.12.001
Piella J, Bastús NG, Puntes V (2016) Size-controlled synthesis of Sub-10-nanometer citrate-stabilized gold nanoparticles and related optical properties. Chem Mater 28:1066–1075. https://doi.org/10.1021/acs.chemmater.5b04406
Théry C, Witwer KW, Aikawa E et al (2018) Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 7:1535750. https://doi.org/10.1080/20013078.2018.1535750
Henry T, Pommier S, Journet L et al (2004) Improved methods for producing outer membrane vesicles in gram-negative bacteria. Res Microbiol 155:437–446. https://doi.org/10.1016/j.resmic.2004.04.007
Monod J (1949) The growth of bacterial cultures. Annu Rev Microbiol 3:371–394
Tashiro Y, Ichikawa S, Shimizu M et al (2010) Variation of physiochemical properties and cell association activity of membrane vesicles with growth phase in Pseudomonas aeruginosa. Appl Environ Microbiol 76:3732–3739. https://doi.org/10.1128/AEM.02794-09
Welton JL, Webber JP, Botos LA et al (2015) Ready-made chromatography columns for extracellular vesicle isolation from plasma. J Extracell Vesicles 4:1–9. https://doi.org/10.3402/jev.v4.27269
Karuppasamy L, Chen CY, Anandan S, Wu JJ (2017) High index surfaces of Au-nanocrystals supported on one-dimensional MoO3-nanorod as a bi-functional electrocatalyst for ethanol oxidation and oxygen reduction. Electrochim Acta 246:75–88. https://doi.org/10.1016/j.electacta.2017.06.040
Krassowska W, Filev PD (2007) Modeling electroporation in a single cell. Biophys J 92:404–417. https://doi.org/10.1529/biophysj.106.094235
Cai W, Gao T, Hong H, Sun J (2008) Applications of gold nanoparticles in cancer nanotechnology. Nanotechnol Sci Appl 1:17–32
Gibson JD, Khanal BP, Zubarev ER (2007) Paclitaxel-functionalized gold nanoparticles. J Am Chem Soc 129:11653–11661. https://doi.org/10.1021/ja075181k
Aryal S, Grailer JJ, Pilla S et al (2009) Doxorubicin conjugated gold nanoparticles as water-soluble and pH-responsive anticancer drug nanocarriers. J Mater Chem 19:7879–7884. https://doi.org/10.1039/b914071a
Goodman CM, McCusker CD, Yilmaz T, Rotello VM (2004) Toxicity of gold nanoparticles functionalized with cationic and anionic side chains. Bioconjug Chem 15:897–900. https://doi.org/10.1021/bc049951i
van der Ley P, van den Dobbelsteen G (2011) Next-generation outer membrane vesicle vaccines against Neisseria meningitidis based on nontoxic LPS mutants. Hum Vaccin 7:886–890. https://doi.org/10.4161/hv.7.8.16086
Dauros Singorenko P, Chang V, Whitcombe A et al (2017) Isolation of membrane vesicles from prokaryotes: a technical and biological comparison reveals heterogeneity. J Extracell Vesicles 6:1324731. https://doi.org/10.1080/20013078.2017.1324731