Electron microscopy of endothelial cells in culture: II. Scanning electron microscopy and OTOTO impregnation method
Tóm tắt
A method is described whereby endothelial cells are treated with several cycles of osmium and thiocarbohydrazide then critical point dried. Cells grown on Formvar-coated gold grids can be examined directly by transmission electron microscopy, cells grown on glass strips are mounted on stubs for scanning electron microscopy and are scanned without further coating with gold or palladium.
Tài liệu tham khảo
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