Electrochemical reduction of immunoglobulins specific reduction of the inter‐heavy chain disulfide bridges of igg

European Journal of Immunology - Tập 3 Số 9 - Trang 537-542 - 1973
C. Rivat1, Marc Fontaine1, C Ropartz2, Claude Caullet3
1Groupe de Recherches U 78 de l'INSERM sur la Génétique des protéines humaines, Mont‐Saint‐Aignan
2Centre Départemental de Transfusion Sanguine et de Génétique Humaine, Mont‐Saint‐Aignan
3Bois‐Guillaume and Institut National Supérieur de Chimie Industrielle de Rouen, Mont‐Saint‐Aignan

Tóm tắt

AbstractElectrochemical reduction of IgG disulfide bridges has been studied and the number of disulfide bridges reduced determined by several methods: coulometry and polarographic titrations at the mercury electrode with electroactive alkylating agents, such as iodoacetamide and N‐ethylmaleimide. The three methods give identical results. Determination and localization of reduced disulfide bridges examined in the four IgG subclasses show that the reduction occurs exclusively on the interheavy chain disulfide bridges.

Từ khóa


Tài liệu tham khảo

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Thông tin
Thông tin xuất bản

European Journal of Immunology

Tập 3 Số 9

537-542

Thông tin tác giả