Electrochemical Sensing of DNA Using Gold Nanoparticles

Electroanalysis - Tập 19 Số 7-8 - Trang 743-753 - 2007
María Teresa Castañeda1,2,3, Salvador Alegret1, Arben Merkoçi2
1Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalonia, Spain
2Institut Català de Nanotecnologia, Campus UAB, 08193 Bellaterra, Barcelona, Catalonia, Spain
3On leave from: Departamento de Ciencias Básicas, Universidad Autónoma Metropolitana-Azcapotzalco, 022000, México, D. F., Mexico

Tóm tắt

AbstractThe electrochemical properties of gold nanoparticles (AuNPs) have led to their widespread use as DNA labels. This fact has improved the design strategies for the electrochemical detection of DNA through hybridization event monitoring. The reported DNA hybridization detection modes are based on either AuNP detection after dissolving or the direct detection of the AuNP/DNA conjugates anchored onto the genosensor surface. Various enhancement strategies have been reported so as to improve the detection limit. Most are based on catalytic deposition of silver onto AuNP. Other strategies based on the use of AuNPs as carrier/amplifier of other labels will be also revised. The developed techniques are characterized by sensitivities and specificities that enable further applications of the developed DNA sensors in several fields.

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