Effects of tyrosine289phenylalanine mutation on binding and functional properties of the human tachykinin NK2 receptor stably expressed in Chinese hamster ovary cells

Maggi, 1993, Tachykinin receptors and tachykinin receptor antagonists, J Autonom Pharmacol, 13, 23, 10.1111/j.1474-8673.1993.tb00396.x Regoli, 1994, Receptors and antagonists for substance P and related peptides, Pharmacol Rev, 46, 551 Blount, 1993, Functional nonequivalence of structurally homologous domains of NK1 and NK2 type tachykinin receptors, J Biol Chem, 268, 16388, 10.1016/S0021-9258(19)85432-7 Arkinstall, 1994, Calcium influx and protein kinase C activation mediate arachidonic acid mobilization by the human NK2 receptor expressed in CHO cells, FEBS Lett, 338, 75, 10.1016/0014-5793(94)80119-3 Gether U, Yokota Y, Emonds-Alt X, Breliere JC, Lowe III JA, Snider RM, Nakanishi S and Schwartz TW, Two nonpeptide tachykinin antagonists act through epitopes on corresponding segments of the NK1 and NK2 receptors. Proc Natl Acad Sci USA 90: 6194–6198, 1993. Bhogal, 1994, The ligand binding site of the NK2 receptor, J Biol Chem, 269, 27269, 10.1016/S0021-9258(18)46979-7 Turcatti, 1995, Probing the binding domain of the NK2 receptor with fluorescent ligands: Evidence that heptapeptide agonists and antagonists bind differently, Biochemistry, 34, 3972, 10.1021/bi00012a015 Huang, 1995, Identification of residues involved in ligand binding to the neurokinin-2 receptor, Biochemistry, 34, 10048, 10.1021/bi00031a029 Emonds-Alt, 1992, A potent and selective nonpeptide antagonist of the neurokinin A (NK2) receptor, Life Sci Pharmacol Lett, 50, L101 Catalioto, 1998, MEN 11420 (Nepadutant) a novel glycosylated bicyclic peptide tachykinin NK2 receptor antagonist, Br J Pharmacol, 123, 81, 10.1038/sj.bjp.0701587 Taylor, 1985, The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA, Nucl Acids Res, 13, 8764, 10.1093/nar/13.24.8765 Nakamaye, 1986, Inhibition of restriction endonuclease Ncil cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis, Nucl Acids Res, 14, 9679, 10.1093/nar/14.24.9679 Rotondaro, 1997, High-level expression of cDNA for granulocyte colony-stimulating factor in Chinese hamster ovary cells. Effect of 3′-noncoding sequences, Mol Biotechnol, 7, 231, 10.1007/BF02740814 Urlaub, 1980, Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity, Proc Natl Acad Sci USA, 77, 4216, 10.1073/pnas.77.7.4216 Oldham, 1990, Polyphosphoinositide turnover, 99 Challiss, 1995, Mass measurement of key phosphoinositide cycle intermediates. Methods in Molecular Biology, 167 Bradford, 1976, A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein–dye binding, Anal Biochem, 72, 248, 10.1016/0003-2697(76)90527-3 Munson, 1980, LIGAND, Anal Biochem, 107, 220, 10.1016/0003-2697(80)90515-1 McPherson, 1985, Analysis of radioligand binding experiments, J Pharmacol Meth, 14, 213, 10.1016/0160-5402(85)90034-8 Catalioto, 1998, Independent coupling of the human tachykinin NK2 receptor to phospholipases C and A2 in transfected Chinese hamster ovary cells, Naunyn-Schmiedeberg’s Arch Pharmacol, 358, 395, 10.1007/PL00005270 Maggi, 1997, The dual nature of the tachykinin NK1 receptor, Trends Pharmacol Sci, 18, 351, 10.1016/S0165-6147(97)90663-5 Patacchini, 1994, Different mechanism of tachykinin NK2 receptor blockade by SR 48968 and MEN 10627 in the guinea-pig isolated gallbladder and colon, Eur J Pharmacol, 271, 111, 10.1016/0014-2999(94)90271-2 Maggi, 1993, In vivo and in vitro pharmacology of SR 48,968, a non-peptide tachykinin NK2 receptor antagonist, Eur J Pharmacol, 234, 83, 10.1016/0014-2999(93)90709-Q Maggi, 1994, Comparison of tachykinin NK1 and NK2 receptors in the circular muscle of the guinea-pig ileum and proximal colon, Br J Pharmacol, 112, 150, 10.1111/j.1476-5381.1994.tb13045.x Emonds-Alt, 1993, Characterization of the binding sites of [3H]SR 48,968, a potent nonpeptide radioligand antagonist of the NK2 receptor, Biochem Biophys Res Comm, 191, 1172, 10.1006/bbrc.1993.1340