Effects of the organophosphorous methyl parathion on the branchial epithelium of a freshwater fish Metynnis roosevelti

Brazilian Archives of Biology and Technology - Tập 46 Số 3 - Trang 361-372 - 2003
Marcelo Rubens Machado1, Edith Fanta1
1Universidade Norte do Paraná, Brazil

Tóm tắt

Gills are vital structures for fish, since they are the main site for gaseous exchange as well as partially responsible for osmorregulation, acid-basic balance, excretion of nitrogenous compounds and taste. Chemicals in the water may alter the morphology of branchial cells of fish that are, therefore, a useful model for environmental impact and ecotoxicology studies. In order to investigate the effects of an organophosphorous compound, methyl parathion, on the gills of the fish, samples of Metynnis roosevelti were exposed to lethal (7ppm) and sublethal (1ppm) doses of Mentox 600 CE. Through light and scanning electron microscopy, shrinking of the branchial epithelium, followed by detachment and hyperplasia were observed. Externally, the branchial filaments presented the gradual disappearance of microridges. Even in sublethal doses, the organophosphorous reduced the health and fitness of these fish, as consequence of secondary effects derived from changes in the branchial epithelium, impairing oxygenation and ionic balance of the organism.

Từ khóa


Tài liệu tham khảo

Abel P. D., 1976, Toxic action of several lethal concentrations of an anionic detergent on the gills of the brown trout (Salmo trutta L.), J. Fish. Biol., 9, 441, 10.1111/j.1095-8649.1976.tb04692.x

Arillo A., 1979, The gill sialic acid content as an index of environmental stress in rainbow trout, Salmo gairdneri Richardson, J. Fish. Biol., 15, 405, 10.1111/j.1095-8649.1979.tb03623.x

Barberá C., 1976

Bettex-Galland M., 1973, Contractile filamentous material in the pillar cells of fish gills, J. Cell. Sci., 13, 359, 10.1242/jcs.13.2.359

Bolis L., 1980, Interactions between vascular actions of detergent and catecholamines in perfused gills of european eel, Anguilla anguilla L. and brown trout, Salmo trutta L., J. Fish. Biol., 16, 61, 10.1111/j.1095-8649.1980.tb03686.x

Bull D., 1986

Culling C. F., 1985

Datta-Munshi J. S., 1986, Scanning electron microscopy of the respiratory organs of juvenile and adult climbing perch, Anabas testudineus, Japanase Journal of Ichthyology, 33, 39, 10.1007/BF02905557

Davis P. W., 1971, Na+K+-activated-ATPase inhibition in rainbow trout: a site for organochlorine pesticide toxicity?, Comp. Biochem. Physiol., 40B, 823

Daye P. G., 1980, Structural alterations in embryos and alevins of the Atlantic salmon, Salmo salar L., induced by continuous or short-term exposure to acidic levels of pH, Can. J. Zool., 58, 27, 10.1139/z80-004

Eiras-Stofella D. R., 2001, Surface ultrastructure of the gills of the mullets Mugil curema, M. liza and M. platanus (Mugilidae, Pisces), Journ. Morphol., 247, 122, 10.1002/1097-4687(200102)247:2<122::AID-JMOR1007>3.0.CO;2-5

1986

Epstein F. H., 1980, Role of Na-K-ATPase in chloride cell function, Am. J. Physiol., 238, 246

Evans D. H., 1987, The fish gill: site of action and model for toxic effects of environmental pollutants, Environmental Health Perspectives, 71, 47, 10.1289/ehp.877147

Evans D. H., 1986, Gill ammonia transport, Journal of Experimental Zoology, 239, 17, 10.1002/jez.1402390104

Evans D. H., 1982, Fish gill ionic transport: methods and models, Biol. Bull., 163, 108, 10.2307/1541502

Fanta E., 1995, Influence of the background color on the behavior of the fish Oreochromis niloticus (Cichlidae), Arq. Biol. Tecnol., 38, 1237

Fanta E., 1989, The effect of environmental oxygen and carbon dioxide levels on the tissue oxygenation and the behaviour of Antarctic fish, Comp. Biochem. Physiol, 93A, 819, 10.1016/0300-9629(89)90507-0

Fanta E., 1995, Gill structure of the Antartic fishes Notothenia (Gobionotothen) gibberifrons and Trematomus newnesi (Nototheniidae) stressed by salinity changes and some behavioral consequences, Antartic Record (Nankyoku Shiryô), 39, 25

Fanta-Feofiloff E., 1986, Effect of salinity on the behaviour and oxygen consumption of Mugil curema (Pisces, Mugilidae), Physiol. and Behav., 36, 1029, 10.1016/0031-9384(86)90475-0

Flik G., 1993, Calcium transport in fish gills and intestine, J. Exp. Biol., 184, 17, 10.1242/jeb.184.1.17

Goldstein L., 1982, 193

Gonzales R. J., 1992, The relationship between oxygen consuption and ion loss in a freshwater fish, J. Exp. Biol., 163, 317, 10.1242/jeb.163.1.317

Goss G. G., 1992, Mechanisms of ion and acid-base regulation at the gills of freshwater fish, Journal of Experimental Zoology, 263, 143, 10.1002/jez.1402630205

Hossler F. E., 1980, Gill arch of the mullet, Mugil cephalus: III. Rate of response to salinity change, Am. J. Physiol., 238, 160

Hossler F. E., 1986, The gill arch of the striped bass, Morone saxatilis. I: Surface ultrastructure, J. Sci. Microsc. Cytol., 18, 519

Hughes G. M., 1966, Species variation in gas exchange, Proceedings of the Royal Society of Medicine, 59, 494, 10.1177/003591576605900609

Hughes G. M., 1979, Scanning electron microscopy of the respiratory surfaces of trout gills, J. Zool., 188, 443

Hughes G. M., 1982, 1

Kawall H. G., 1993

Khan R. A., 1984, Histopathological effects of crude oil on Atlantic cod following chronic exposure, Can. J. Zool., 62, 2038, 10.1139/z84-298

Laurent P., 1991, Environmental effects on fish gill morphology, Physiological Zoology, 64, 4, 10.1086/physzool.64.1.30158511

Lin H., 1991, Evidence for the presence of an electrogenic proton pump on the trout gill epithelium, J. Exp. Biol., 161, 119, 10.1242/jeb.161.1.119

Lindesjöö E, 1994, Histopathology of skin and gills of fish in pulp mill effluents, Dis. Aquat. Org., 18, 81, 10.3354/dao018081

Lock R. A. C., 1981, Effects of mercuric chloride and methylmercuric chloride on mucus secretion in rainbow trout, Salmo gairdneri Richardson, Comp. Biochem. Physiol., 69 C, 67

Luvizotto M. F., 1994

Magor B. G., 1988, Gill histopathology of juvenile Oncorhynchus kisutch exposed to suspended wood debris, Can. J. Zool., 66, 2164, 10.1139/z88-323

Mallatt J., 1985, Fish gill structural changes induced by toxicants and other irritants: a statistical review, Can. J. Fish. Aquat. Sci., 42, 630, 10.1139/f85-083

Mallatt J., 1985, Ultrastructural effects of 3-trifluor-4-nitrophenol on gills of the larval lamprey Petromyzon marinus, Can. J. Zool., 63, 155, 10.1139/z85-024

Matthiessen P., 1973, The effects of dissolved zinc on the gills of the stickleback Gasterosteus aculeatus L., J. Fish. Biol., 5, 607, 10.1111/j.1095-8649.1973.tb04494.x

McDonald D. G., 1983, The effects of H+ upon the gills of freshwater fish, Can. J. Zool., 61, 691, 10.1139/z83-093

McDonald D. G., 1991, Gill design in freshwater fishes: interrelationships among gas exchange, ion regulation, and acid-base regulation, Physiological Zoology, 64, 103, 10.1086/physzool.64.1.30158515

McKim J. M., 1991, Environmental impacts on the physiological mechanisms controlling xenobiotic transfer across fish gills, Physiological Zoology, 64, 39, 10.1086/physzool.64.1.30158513

Mitz S. V., 1985, Sewage effluent biomonitoring. I: Survival, growth, and histopathological effects in channel catfish, Ecotoxicology and Environmental Safety, 10, 22

Mueller M. E., 1991, Nature and time course of acclimation to aluminum in juvenile brook trout (Salvelinus fontinalis). II: gill histology, Can. J. Fish. Aquat. Sci., 48, 2016, 10.1139/f91-240

Nowak B., 1992, Histological changes in gills induced by residues of endossulfan, Aquatic Toxicology, 23, 65, 10.1016/0166-445X(92)90012-C

Oliveira Ribeiro C. A., 1994

Oronsaye J. A. O., 1984, The effect of dissolved cadmium on the chloride cells of the gills of the stickleback, Gasterosteus aculeatus L., J. Fish. Biol., 25, 253, 10.1111/j.1095-8649.1984.tb04872.x

Randall D., 1982, 173

Rankin J. C., 1982, 207

Rao K. S. P., 1981, Lipid derivatives in the tissues of the freshwater teleost, Saurotherodon mossambicus (alias Tilapia mossambica) (Peters) - effect of methyl parathion, Proc. Indian Natn. Sci. Acad., 47B, 53

Rios F. S., 1998, Morphology of gill rackers and their ecological function in feeding of the Antarctic fish Notothenia neglecta Nybelin, Antarctic Record (Nankyoku Shiryô), 42, 131

Rodrigues E. L., 1998, Liver histopathology of the fish Brachydanio rerio after acute exposure to sublethal levels of the organophosphate Dimetoate 500, Revista Brasileira de Zoologia, 15, 441, 10.1590/S0101-81751998000200014

Romão S., 2001, Ionic regulation and Na,K-ATPase activity in gills and kidney of the aglomerular Antarctic fish Notothenia neglecta upon exposure to seawater dilution, Journ. Fish Biol., 55

Sayer M. D. J., 1987, The relative importance of the gills to ammonia and urea excretion in five seawater and one freshwater teleost species, J. Fish Biol., 31, 561, 10.1111/j.1095-8649.1987.tb05258.x

Schimid O. J., 1961, Action of a detergent (dodecylbenzenesulphonate) on the gills of the trout, Nature, 192, 675, 10.1038/192675a0

Silva H. C., 1993, Sublethal effects of the organophosphate Folidol 600 (Methyl parathion) on Callichthys callichthys (Pisces, Teleostei), Comparative Biochemistry and Physiology, 105C, 197

Skidmore J. F., 1970, Respiration and osmoregulation in rainbow trout with gills damaged by zinc sulphate, J. Exp. Biol., 52, 481, 10.1242/jeb.52.2.481

Smart G., 1976, The effect of ammonia exposure on gill structure of the rainbow trout (Salmo gairdneri), J. Fish. Biol., 8, 471, 10.1111/j.1095-8649.1976.tb03990.x

Soderberg R. W., 1984, Histology of cultured channel catfish, Ictalurus punctatus (Rafinesque), J. Fish. Biol., 24, 683, 10.1111/j.1095-8649.1984.tb04839.x

Sprage J. P., 1973, The abc's of pollutant bioassay using fish, 6

Stoker P. W., 1985, Pathology of gill and liver tissues from two genera of fishes exposed to two coal-derived materials, J. Fish. Biol., 27, 31, 10.1111/j.1095-8649.1985.tb04007.x

Verbost P. M., 1994, Kinetics of ATP and Na+ gradient driven Ca+2 transport in basolateral membranes from gills of freshwater and seawater adapted tilapia, J. Exp. Biol., 186, 95, 10.1242/jeb.186.1.95

Wendelaar Bonga S. E., 1992, Toxicants and osmorregulation in fish, Netherlands Journal of Zoology, 42, 478