Effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in ovariectomized nude mice

Science China Press., Co. Ltd. - Tập 54 - Trang 72-77 - 2009
Qian Wu1, NianZu Jin1, Jing Yu1, RenChen Zhao1, ZePing Yu2, ShanLei Qiao1, XiaoHe Lu3, ChunWen Zhang1
1Department of Hygienic Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing, China
2Research Institute of General Surgery, Nanjing General Hospital of PLA, Nanjing Military Command, Nanjing, China
3Department of Pharmacy, Nanjing General Hospital of PLA, Nanjing Military Command, Nanjing, China

Tóm tắt

In this study, we explored the effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in ovariectomized athymic mice. The ovariectomized athymic mice were implanted with MCF-7 cells. They were fed with low, moderate and high doses of soy isoflavone extracts, at dietary concentrations of 6.25, 12.5 and 25 g/kg, respectively. The expression of ki-67 was detected by immunohistochemistry. The pS 2 expression in tumors was analyzed by real-time PCR. Estrogen level in the serum was measured by chemiluminescence enzyme immunoassay. Compared with the control group, dietary soy isoflavone extracts had a significant stimulatory effect on MCF-7 tumor growth in mice (P < 0.05). The ki-67 and pS 2 mRNA expressions in tumors were significantly increased by 6.25 and 12.5 g/kg dose of soy isoflavone extracts (P < 0.05). And, estrogen level in serum of 6.25 and 12.5 g/kg dose groups was higher than that of control group (P > 0.05). In conclusion, in the tested dietary concentration range soy isoflavone extracts had a stimulatory effect on tumor growth. 6.25 and 12.5 g/kg doses of soy isoflavone extracts can increase the cell proliferation in tumors and induce estrogen-responsive pS2 expression.

Tài liệu tham khảo

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