Effects of salinity, pH and growth phase on the protein productivity by Dunaliella salina

Journal of Chemical Technology and Biotechnology - Tập 94 Số 4 - Trang 1032-1040 - 2019
Yixing Sui1, Siegfried E. Vlaeminck1
1Research Group of Sustainable Energy, Air and Water Technology, Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium

Tóm tắt

AbstractBACKGROUNDMicroalgae have long been adopted for use as human food, animal feed and high‐value products. For carotenogenesis, Dunaliella salina is one of the most studied microalgae, yet its protein synthesis has been limitedly reported. In this study, D. salina was cultivated at different NaCl and pH levels to optimize its protein productivity.RESULTSThe biomass protein content followed an increase–decrease pattern throughout the growth phases, with a maximum in the exponential phase (60–80% over ash‐free dry weight). Adversely, the biomass pigment contents were at relatively stable levels (around 0.5% carotenoids, 1.3% chlorophyll a and 0.5% chlorophyll b over ash‐free dry weight). Among the tested conditions (1–3 mol L−1 salinity, pH 7.5–9.5), the highest protein productivity (43.5 mg L−1 day−1) was achieved at 2 mol L−1 salinity and pH 7.5 during the exponential phase, which surpassed others by 16–97%. Additionally, table salts were tested to be equivalent and cost‐efficient salt sources for the growth medium.CONCLUSIONThis study highlighted the suitability of D. salina as a protein source, providing guidelines for 70% cheaper medium formulation in the lab and for maximum protein productivity at larger scale. © 2018 Society of Chemical Industry

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Tài liệu tham khảo

Corcoran E, 2010, Sick Water? The Central Role of Wastewater Management in Sustainable Development, 49

10.1016/j.tifs.2012.07.002

10.1016/S0734-9750(00)00045-8

10.1016/j.tifs.2014.12.004

Enzing C, 2014, Microalgae‐based Products for the Food and Feed Sector: an Outlook for Europe, 9

10.1201/b10300-2

10.1007/s10811-013-9983-9

10.1016/j.biotechadv.2006.11.002

10.1007/BF00938962

Finney KF, 1984, Use of algae Dunaliella as a protein supplement in bread, Cereal Chem, 61, 402

10.1128/AEM.31.4.602-604.1976

10.1080/10498850.2012.759634

10.1016/j.psep.2017.04.010

10.1007/s00253-011-3399-8

10.1007/s10811-007-9255-7

LamersPP Metabolomics of carotenoid accumulation inDunaliella salina. PhD thesis Wageningen University (2011).

10.1111/j.0022-3646.1980.00028.x

10.1046/j.1529-8817.2001.01052.x

Borowitzka MA, 1988, Micro‐algal Biotechnology, 456

10.1098/rstl.1825.0026

10.1128/AEM.56.6.1875-1881.1990

10.1016/0003-2697(78)90586-9

10.1016/0076-6879(87)48036-1

10.1007/s10098-016-1159-2

10.1016/S0031-9422(00)80305-2

10.1016/0044-8486(89)90057-4

10.1023/A:1007973319348

10.1016/S0044-8486(98)00278-6

10.1007/BF02182737

10.1007/BF02186231

10.1016/j.copbio.2010.03.005

10.1186/s13068-017-0839-4

10.1002/er.3967

10.1016/j.energy.2018.01.042

10.1016/j.biotechadv.2012.02.005

10.1023/A:1007921728300

Borowitzka MA, 2012, Algae for Biofuels and Energy, 255

10.1016/j.rser.2009.10.009

10.1007/BF00003552

10.1016/j.jbiotec.2004.07.010

10.1016/j.plaphy.2016.05.021

10.1016/j.biotechadv.2010.08.005

10.1080/10408390701279749

10.1007/s00253-008-1681-1

10.1016/j.btre.2016.04.001

10.1016/j.tibtech.2008.07.002

10.1007/s10811-011-9711-2

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Journal of Chemical Technology and Biotechnology

Tập 94 Số 4

1032-1040

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