Effects of photobiomodulation at different wavelengths on orthodontically induced root resorption in orthodontic retention period: a micro-CT and RT-PCR study
Tóm tắt
The aim of this study was to evaluate and compare the reparative and inhibitory effects of single wavelength photobiomodulation (SW-PBM) and of cumulative increased wavelength photobiomodulation (CW-PBM) on orthodontically induced inflammatory root resorption (OIIRR). Thirty-three Wistar albino rats were divided into five groups: untreated group (negative control), only relapse group (positive control-1), only retention group (positive control-2), SW-PBM group (650 nm, 100 mW/cm2), and CW-PBM group (532–650–940 nm, 100 mW/cm2). Orthodontic tooth movement was induced experimentally in rats for 10 days with an applied force of 50 cN; retention and therapeutic approaches were performed concurrently. At the end of the experiment, maxillary quadrants were prepared for micro-CT analysis and real-time polymerase chain reaction (RT-PCR) analysis. After the Shapiro-Wilk normality test, Kruskal-Wallis test followed post hoc Bonferroni test and paired samples t test was used for statistical evaluation of the data. Resorption lacunae volume (p < 0.001), number of resorption lacunae (p < 0.05), and percentage of the resorption (PR) lacunae (p < 0.001) decreased with PBM applications when compared with the positive control groups, and the mean PR was similar in the negative control group when compared with SW-PBM group. Receptor activator of nuclear factor kappa B ligand (RANKL) levels of the PBM groups were lower (p < 0.05) than those of the positive control groups. Cyclooxygenase-2 (COX-2) expression levels significantly decreased with PBM administration (p < 0.05). No significant change was found in osteoprotegerin (OPG) expression levels and OPG/RANKL ratios (p > 0.05). PBM applications showed marked inhibitory and reparative effects on OIIRR by modulating the RANKL and COX-2 expression levels. However, the effects of the different wavelengths were similar to each other. Graphical abstract
Tài liệu tham khảo
Franzen TJ, Monjo M, Rubert M, Vandevska-Radunovic V (2014) Expression of bone markers and micro-CT analysis of alveolar bone during orthodontic relapse. Orthod Craniofacial Res 17:249–258. https://doi.org/10.1111/ocr.12050
Franzen TJ, Brudvik P, Vandevska-Radunovic V (2013) Periodontal tissue reaction during orthodontic relapse in rat molars. Eur J Orthod 35:152–159. https://doi.org/10.1093/ejo/cjr127
Thilander B (2000) Biological basis for orthodontic relapse. Semin Orthod 6:195–205. https://doi.org/10.1053/sodo.2000.8085
Yoshida Y, Sasaki T, Yokoya K, Hiraide T, Shibasaki Y (1999) Cellular roles in relapse processes of experimentally-moved rat molars. J Electron Microsc 48:147–157. https://doi.org/10.1093/oxfordjournals.jmicro.a023661
Lovatt R, Goonewardenet M, Tennant M (2008) Relapse following orthodontic rotation of teeth in dogs. Aust Orthod J 24:5–9
King GJ, Latta L, Rutenberg J, Ossi A, Keeling SD (1997) Alveolar bone turnover and tooth movement in male rats after removal of orthodontic appliances. Am J Orthod Dentofac Orthop 111:266–275. https://doi.org/10.1016/S0889-5406(97)70184-7
Brezniak N, Wasserstein A (2002) Orthodontically induced inflammatory root resorption. Part I: the basic science aspects. Angle Orthod 72:175–179. https://doi.org/10.1043/0003-3219(2002)072<0175:Oiirrp>2.0.Co;2
Brudvik P, Rygh P (1994) Multi-nucleated cells remove the main hyalinized tissue and start resorption of adjacent root surfaces. Eur J Orthod 16:265–273. https://doi.org/10.1093/ejo/16.4.265
Brudvik P, Rygh P (1995) The repair of orthodontic root resorption: an ultrastructural study. Eur J Orthod 17:189–198. https://doi.org/10.1093/ejo/17.3.189
Fonseca PDA, de Lima FM, Higashi DT, Koyama DFV, de Oliveira Toginho Filho D, Dias IFL, de Paula RS (2013) Effects of light emitting diode (LED) therapy at 940 nm on inflammatory root resorption in rats. Lasers Med Sci 28:49–55. https://doi.org/10.1007/s10103-012-1061-z
Lim HJ, Bang MS, Jung HM, Shin JI, Chun GS, Oh CH (2014) A 635-nm light-emitting diode (LED) therapy inhibits bone resorptive osteoclast formation by regulating the actin cytoskeleton. Lasers Med Sci 29(2):659–670. https://doi.org/10.1007/s10103-013-1363-9
Kim YD, Kim SS, Kim SJ, Kwon DW, Jeon ES, Son WS (2010) Low-level laser irradiation facilitates fibronectin and collagen type I turnover during tooth movement in rats. Lasers Med Sci 25:25–31. https://doi.org/10.1007/s10103-008-0585-8
Kim SJ, Kang YG, Park JH, Kim EC, Park YG (2013) Effects of low-intensity laser therapy on periodontal tissue remodeling during relapse and retention of orthodontically moved teeth. Lasers Med Sci 28:325–333. https://doi.org/10.1007/s10103-012-1146-8
Gul Amuk N, Kurt G, Guray E (2018) Effects of photobiomodulation and ultrasound applications on orthodontically induced inflammatory root resorption; transcriptional alterations in OPG, RANKL, Cox-2: an experimental study in rats. Photomed Laser Surg 36:653–659. https://doi.org/10.1089/pho.2018.4508
de Sousa APC, Santos JN, dos Reis Jr JA, Ramos TA, de Souza J, Cangussú MCT, Pinheiro AL (2010) Effect of LED phototherapy of three distinct wavelengths on fibroblasts on wound healing: a histological study in a rodent model. Photomed Laser Surg 28:547–552. https://doi.org/10.1089/pho.2009.2605
Jahangiri Noudeh Y, Shabani M, Vatankhah N, Hashemian SJ, Akbari K (2010) A combination of 670 nm and 810 nm diode lasers for wound healing acceleration in diabetic rats. Photomed Laser Surg 28:621–627. https://doi.org/10.1089/pho.2009.2634
Avci P, Gupta A, Sadasivam M, Vecchio D, Pam Z, Pam N, Hamblin MR (2013) Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg 32:41–52
Ng D, Chan AK, Papadopoulou AK, Dalci O, Petocz P, Darendeliler MA (2018) The effect of low-level laser therapy on orthodontically induced root resorption: a pilot double blind randomized controlled trial. Eur J Orthod 40:317–325. https://doi.org/10.1093/ejo/cjx065
Brudvik P, Rygh P (1993) The initial phase of orthodontic root resorption incident to local compression of the periodontal ligament. Eur J Orthod 15:249–263. https://doi.org/10.1093/ejo/15.4.249
Tuner J, Hode L (2002) Laser therapy: clinical practice scientific background. Sweden: Grangesberg: Prima Books; 571 p
Martorelli M, Ausiello P, Morrone R (2014) A new method to assess the accuracy of a cone beam computed tomography scanner by using a non-contact reverse engineering technique. J Dent 42:460–465. https://doi.org/10.1016/j.jdent.2013.12.018
Gul Amuk N, Karsli E, Kurt G (2019) Comparison of dental measurements between conventional plaster models, digital models obtained by impression scanning and plaster model scanning. Int Orthod 17:151–158. https://doi.org/10.1016/j.ortho.2019.01.014
Zhao N, Liu Y, Kanzaki H, Liang W, Ni J, Lin J (2012) Effects of local osteoprotegerin gene transfection on orthodontic root resorption during retention: an in vivo micro-CT analysis. Orthod Craniofacial Res 15:10–20. https://doi.org/10.1111/j.1601-6343.2011.01532.x
Pires Oliveira DA, de Oliveira RF, Zangaro RA, Soares CP (2008) Evaluation of low-level laser therapy of osteoblastic cells. Photomed Laser Surg 26:401–404. https://doi.org/10.1089/pho.2007.2101
Luger EJ, Rochkind S, Wollman Y, Kogan G, Dekel S (1998) Effect of low-power laser irradiation on the mechanical properties of bone fracture healing in rats. Lasers Surg Med 22:97–102. https://doi.org/10.1002/(SICI)1096-9101(1998)22:2%3C97::AID-LSM5%3E3.0.CO;2-R
Kawasaki K, Shimizu NV (2000) Effects of low-energy irradiation on bone remodelling during experimental tooth movement in rats. Lasers Surg Med 26:282–291. https://doi.org/10.1002/(SICI)1096-9101(2000)26:3%3C282::AID-LSM6%3E3.0.CO;2-X
Altan AB, Bicakci AA, Mutaf HI, Ozkut M, Inan VS (2015) The effects of low-level laser therapy on orthodontically induced root resorption. Lasers Med Sci 30:2067–2076. https://doi.org/10.1007/s10103-015-1717-6
Youssef M, Ashkar S, Hamade E, Gutknecht N, Lampert F, Mir M (2008) The effect of low-level laser therapy during orthodontic movement: a preliminary study. Lasers Med Sci 23(1):27–33. https://doi.org/10.1007/s10103-007-0449-7
Fujita S, Yamaguchi M, Utsunomiya T, Yamamoto H, Kasai K (2008) Low-energy laser stimulates tooth movement velocity via expression of RANK and RANKL. Orthod Craniofacial Res 11(3):143–155. https://doi.org/10.1111/j.1601-6343.2008.00423.x
Ekizer A, Uysal T, Güray E, Akkuş D (2015) Effect of LED-mediated-photobiomodulation therapy on orthodontic tooth movement and root resorption in rats. Lasers Med Sci 30:779–785. https://doi.org/10.1007/s10103-013-1405-3
Gonzales C, Hotokezaka H, Karadeniz EI, Miyazaki T, Kobayashi E, Darendeliler MA, Yoshida N (2011) Effects of fluoride intake on orthodontic tooth movement and orthodontically induced root resorption. Am J Orthod Dentofac Orthop 139:196–205. https://doi.org/10.1016/j.ajodo.2009.05.029
Gomes MF, da Graças Vilela Goulart M, Giannasi LC, Hiraoka CM, de Fátima Santana Melo G, de Sousa AGV, Nóbrega CJP, Zangaro RA, Salgado MAC (2017) Effects of the GaAlAs diode laser (780 nm) on the periodontal tissues during orthodontic tooth movement in diabetes rats: histomorphological and immunohistochemical analysis. Lasers Med Sci 32(7):1479–1487. https://doi.org/10.1007/s10103-017-2268-9
Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354. https://doi.org/10.1016/S8756-3282(97)00294-9
Higashi DT, Andrello AC, Tondelli PM, de Oliveira Toginho Filho D, de Paula RS (2017) Three consecutive days of application of LED therapy is necessary to inhibit experimentally induced root resorption in rats: a microtomographic study. Lasers Med Sci 32:181–187. https://doi.org/10.1007/s10103-016-2100-y
Karu TI (2008) Mitochondrial signaling in mammalian cells activated by red and near-IR radiation. Photochem Photobiol 84:1091–1099. https://doi.org/10.1111/j.1751-1097.2008.00394.x
Wierzbicki T, El-Bialy T, Aldaghreer S, Li G, Doschak M (2009) Analysis of orthodontically induced root resorption using micro-computed tomography (micro-CT). Angle Orthod 79:91–96. https://doi.org/10.2319/112107-546.1
Fekrazad R, Asefi S, Eslaminejad MB, Taghiar L, Bordbar S, Hamblin MR (2019) Photobiomodulation with single and combination laser wavelengths on bone marrow mesenchymal stem cells: proliferation and differentiation to bone or cartilage. Lasers Med Sci 34:115–126. https://doi.org/10.1007/s10103-018-2620-8
Matys J, Świder K, Grzech-Leśniak K, Dominiak M, Romeo U (2019) Photobiomodulation by a 635nm diode laser on peri-implant bone: primary and secondary stability and bone density analysis—a randomized clinical trial. BioMed Res Int 22;2019:2785302. https://doi.org/10.1155/2019/2785302
Coon D, Gulati A, Cowan C, He J (2007) The role of cyclooxygenase-2 (COX-2) in inflammatory bone resorption. J Endod 33(4):432–436. https://doi.org/10.1016/j.joen.2006.12.001
Xu M, Deng T, Mo F, Deng B, Lam W, Deng P, Zhang X, Liu S (2009) Low-intensity pulsed laser irradiation affects RANKL and OPG mRNA expression in rat calvarial cells. Photomed Laser Surg 27:309–315. https://doi.org/10.1089/pho.2008.2283
Fekrazad R, Ghuchani MS, Eslaminejad MB, Taghiyar L, Kalhori KAM, Pedram MS, Abrahamse H (2015) The effects of combined low-level laser therapy and mesenchymal stem cells on bone regeneration in rabbit calvarial defects. J Photochem Photobiol B 151:180–185. https://doi.org/10.1016/j.jphotobiol.2015.08.002